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Methods The amino modified wild-type and mutant probes of 16 loci ˇ1888, 3200, 3206, 3243, 3290, 3316, 3394, 3593, 3426, 12026, 12258, 14577, 14693, 14709, 16189, 16223ˇ in mitochondrial genome were immobilized onto the activated slides with aldehyde surface using Biochip Arrayer, then they were incubated overnight in a humid chamber at room temperature.

方法将氨基修饰的16个常见易感基因位点ˇ1888ˇ3200ˇ3206ˇ3243ˇ3290ˇ3316ˇ3394ˇ3593ˇ3426ˇ12026ˇ12258ˇ14577，14693ˇ14709ˇ16189ˇ16223ˇ的野生型和突变型探针ˇ通过Packard芯片点样仪点样于醛基修饰的基片上ˇ室温保湿过夜固定。

These were incubated with shaking for 4 days at 32 C, and the sporulated culture was kept at 45 C overnight to allow autolysis of vegetative cells.

这些培养物在32C下摇动着培养了了4天，孢子培养是保持在45C下一整夜，以便植物细胞自溶。

Balut are duck eggs that have been incubated until the fetus is all feathery and beaky, and then boiled alive.

巴鲁特是孵化到一半的鸭蛋，蛋里的小鸭子刚刚长出羽毛和嘴巴，却被活生生煮熟。

Via changing the concentration of beef extract and peptone, sensor had the most frequency response in culture medium CM2-2 incubated with 2×10^5 cells/mL S. aureus.

通过改变牛肉膏和蛋白胨的浓度，得出传感器在含有2×10^5cells/mL金黄色葡萄球菌的培养基CM2-2中共振频率响应最大。

Results The optimum preparation formulation:drug/PC:1∶30,liposomes were pH 7.4 outside and incubated at 30℃.The encapsulation rate of Vinorelbine Bitartrate in liposomes was above 85%.

结果 制备重酒石酸长春瑞滨脂质体的最佳条件是药物与磷脂的质量比为1∶30，胆固醇与磷脂质量比为1∶4，脂质体外水相pH值最终调至7.4，孵育温度为30℃，此时的包封率可以达到85%以上。

Reerse transcription was performed with 3 g of total RNA, random hexamer (Amersham Bioscience, Buckinghamshire, United Kingdom), and Superscript II reerse transcriptase at conditions recommended by the manufacture, and then incubated with 1l of RNase at 37oC for 20 minutes.

再根据生产说明介绍的条件，从中选取3克RNA，随机六聚物（英国白金汉郡Amersham生物科学公司生产的）以及逆转录标记II进行逆转录，用1LRNA酶(Initrogen公司生产)在37度的环境中孵化20分钟。

The membrane of sperm caput and principal piece were damaged and the cytoplasm of them were swelling after incubated 5 hours with peritoneal fluid of EM .

1。轻度EM不孕患者腹腔液中TNF-α的含量高于正常人，且与病情严重程度相关，推测其可能在EM早期的病情发展中起作用。

Methods The mouse peritoneal macrophages were incubated with latex beads in 24 well plate in cell culture incubator.

目的 建立和完善可靠、敏感、稳定的评价腹腔巨噬细胞吞噬功能方法。

Methods :Choriocarcinoma JAR cells were incubated with different concentrations of genistein.

在绒毛膜癌细胞JAR的培养基中加入不同浓度的genistein，每隔24h吸培养上清，测人绒毛膜促性腺激素的含量。

After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定：采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒，将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合，靶细胞被裂解后，释放的乳酸脱氢酶与底物反应显色，酶标仪测定吸光度，吸光度与裂解程度成正比，按照公式计算百分裂解率。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了