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Then 15--20 zona-free hamster ova were added to each sperm aliquots, and the inixture was incubated in a CO_2 incubator for 3 hours.

空白对照组和血清对照组分别用BWW液和灭活小鼠血清代替人精子单克隆抗体。

Screening of thermal stabilizers for HRPHRP powder was dissolved in distilled water and mixed with additives. Aliquots of enzyme solution were added to a microplate and incubated in the air under thermal stress or room temperature.

HRP热稳定剂的筛选：HRP干粉用双蒸水溶解(0.1μg/mL)，与作为酶稳定剂的各组分以一定比例混溶后，于96孔板中点样，在加热或室温条件下的空气中存放。

The reagents are incubATed AT 37℃ for a further 30 minutes and the plATes are subsequently centrifuged and read.

37℃温育30分钟，然后离心并读数。至少在大陆部分的板块可能很厚，这一证据长期以来就提出来了。

Mutans was purified from recombinant bacteria. GtfD was incubated in buffered-KCl solution with dextran and sucrose containing sucrose-［14C-glucose ］. The bound activity of GtfD was measured with a scintillation counter. The effects of peroxidase system and its components on the activity of GtfD were examined by incubating different components separately and together with GtfD.

变链菌GtfD由重组菌提纯， GtfD与含葡聚糖和蔗糖（含C14标记葡萄糖）的氯化钾缓冲液孵育，经闪烁计数器测定GtfD活性；过氧化物酶系统及其组份对GtfD活性的影响，通过将这些组份单独或共同和GtfD孵育得以测定。

Both NO donors and PKG activator treatment induced high levels of NO, inducible nitric oxide synthase, and PKG in HG-incubated cells.

如果在高糖环境中以NO供给者与PKG活化剂刺激肾纤维母细胞，则会诱发高浓度NO表现，且iNOS与PKG蛋白质的合成量也会增加。

Methods Platelet aggregations induced by ADP, AA and PAF, respectively, were measured by turbidimetric method after platelet incubated with 5'-nucleotidase.

采用比浊法测定白唇竹叶青蛇毒5'-核苷酸酶对二磷酸腺苷、花生四烯酸、血小板活化因子诱导血小板聚集的影响。

MethodsHepatocyte injury model was induced by CCl4 and the injured hepatocytes were incubated with rat serum obtained after oral administration of CWC. The hepatocyte proliferation rate and alanine aminotransferase concentration in cell culture medium were determined. 446 patients with chronic hepatitis were divided into experimental group and control group treated by Hu Gan Tablets, HGT） randomly. With physical sign, symptom and hepatic function as main indices, the therapeutic effect of CWC on patients with chronic hepatitis was evaluated.

方法以SD大鼠为供体制备复方五仁醇胶囊含药血清，CCl4诱导大鼠原代培养肝细胞损伤模型，采用血清药理学方法考察含药血清对损伤肝细胞增值和培养上清液中丙氨酸氨基转移酶的影响；将446例慢性乙型肝炎患者随机分成治疗组和对照组，以临床症状、体征、肝功能为主要指标，考察复方五仁醇胶囊的治疗效果。

The green recovery plan was incubated at a liberal thinktank, the Centre for American Progress.

绿色恢复计划在自由派智库美国进步中心孕育产生。

The standard biochemical oxygen demand test whereby a sample of the liquid is incubated at 20 degrees C for five days.

标准的生化需氧量试验。其方法是把一份溶液的样品在摄氏20度下培养五天。

Biochemical oxygen demand over five days - BOD5: The standard biochemical oxygen demand test whereby a sample of the liquid is incubated at 20 degrees C for five days.

五天生化需氧量(简写为BOD5)：标准的生化需氧量试验。其方法是把一份溶液的样品在摄氏20度下培养五天。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了