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At first, we investigated the optimal components of culture medium for thereafter purification. We found that the optimal culture medium could produce better yield of red-pigmented antibiotic when 1.5% fructose and 1.5% malt extract included, and incubated at 30℃ under shaking conditions for 6 days.

我们逐步由探讨培养基条件为始，以利后续的生产纯化的进行，发现以1.5%果糖与1.5%麦芽抽出物之液态培养条件下，以30℃震荡培养6天后，有最适红色抗菌物质之产率，并且可减少其他色素的产生，再试著将其纯化。

Different brands of yoghourt were separately cultured in broth and incubated at 37℃, 5% CO2 for 48 h, getting the concentration of lactic acid bacteria through slab counting method.

将不同品牌的酸奶分别接种在MRS培养基中，37℃、5％CO2培养48h，平板计数法分别测得乳酸菌的浓度，选择乳酸菌浓度最高的酸奶品牌作为研究对象。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合，电镜下观察对粒体形态没有影响；TMV与丹皮酚混和后透析可恢复侵染力，表明丹皮酚对病毒粒体并没有直接毒害作用；预先用丹皮酚喷施叶面不影响病毒的侵染，表明丹皮酚未干扰TMV对叶片侵染位点的识别；丹皮酚在低浓度时可阻碍TMV-CP的体外聚合，对TMV-RNA并无体外抑制侵染作用，据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别，或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后，通过透析除去单宁后，可恢复大部分侵染力，表明单宁对病毒粒体并无直接毒害作用；TMV与杠板归和假槟榔种子中提取的单宁分别混合后，电镜下观察粒体呈现明显的聚集现象；TMV外壳蛋白分别与这两种植物单宁混合后，聚丙烯酰胺凝胶电泳中CP泳带滞后；单宁在病毒接种前施用未降低TMV对叶片的侵染能力；这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性，而这种结合是可逆的。

Galactosyl anti CD 3McAb TILs were prepared and then were incubated together with hepatocytes.

因此，寻找术后能有效预防和减少肝癌术后复发的手段至关重要［2］。

Observation of section of filter membrane revealed a stratified cell growth of HPDLF and HGF 2 weeks after inoculation,and TEERs of HPDLF and HGF were 56.14±7.43 and 57.34±7.62Ω·cm~(-2) respectively.Being incubated for 30 minutes, less than 1%of fluorescein sodium reached the inferior filter membrane 2 week after inoculation.

结果：HPDLF及HGF接种1周后细胞基本汇合，到2周时细胞连接紧密且完整，细胞呈复层生长，跨膜电阻分别达56.14±7.4 3及57.34±7.62Ω·cm~(-2)，经荧光素钠孵育30分钟检测细胞旁渗漏小于1%。

The test system is incubated at 37℃for 1 hour prior to the addition of 25 μl of 1.4% standardised sheep red blood cells in VBD sensitised with 5 units of rabbit anti-SRBC.

37℃孵育1小时后加入25微升已致敏的含5个单位的兔抗绵羊红细胞，用VBD液稀释成1.4%的标准绵羊红细胞

Then the HCV subgenomic replicon cell model was incubated with Napp at concentration of 1mg/ml, 10~(-1)mg/ml, 10~(-2)mg/ml, 10~(-3)mg/ml, 10~(-4)mg/ml in cell culture solution and the negative and positive controls were set up correspondingly. After 3 days, the intracellular RNA of HCV subgenomic replicons were detected by FQ-RT PCR.

然后分别取终浓度为1mg/ml、10~(-1)mg/ml、10~(-2)mg/ml、10~(-3)mg/ml、10~(-4)mg/ml的Napp与含HCV亚基因复制子细胞培养液中共同孵育，同时设空白对照组和IFN-α2b阳性对照组，3天后用荧光定量PCR法检测HCV复制子中RNA的含量。

However, neither EGF treatment nor MF exposure caused the receptors clustering when cells were pre-incubated with nystatin.

在对 ASM 分析中，阳性对照组和和磁场处理组中 Cy3标记的 ASM 和 FITC 标记的脂质筏结构产生共定位现象，并且在细胞膜表面形成特定的富集区域，而在假辐照组中基本处于散在状态。

Methods Live attenuated rubella vaccine virus strain RA27/3 was inoculated to the monolayer of human diploid MRC-5 cells at an appropriate MOI and incubated in serum-and protein-free CDM culture medium. The virus liquid was harvested and, after addition of polyvinylpyrrolidone as a stabilizer and protective agent, lyophilized. Perform overall control tests on the prepared vaccine.

将风疹减毒活疫苗毒种RA27/3株按适当比例接种单层人二倍体细胞MRC-5株，使用无血清、无蛋白的CDM培养基培养病毒，收获病毒液，加入聚乙烯吡咯烷酮作为病毒稳定剂，制备疫苗原液，加入冻干保护剂，制备5批冻干疫苗，进行各项检定。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了