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Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞，将重组人睫状神经营养因子加入到细胞中继续培养64h后，用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性，从而定量测定重组人睫状神经营养因子的生物活性。

CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛宁为证明出生后血液循环中存在内皮细胞的前体细胞，从G-CSF动员的成人外周血及脐带血中分离CD34+细胞，将之接种于纤连蛋白与明胶铺板的培养皿上，培养体系中加入重组人内皮细胞生长因子和重组人碱性成纤维细胞生长因子。

After γ ray treatment, two kinds of cells were incubated on 6-well gelatin-coated plate with density of 2.5×104/cm3. hESCs were cultured on HAFi or MEF feeder cells containing β-mercaptoethanol DMEM/F12 and basic fibroblast growth factor.

取人胚胎干细胞，分别接种在小鼠胚胎成纤维细胞或永生化人成纤维细胞饲养层上，加入含β-巯基乙醇的DMEM/F12培养基，使用前添加碱性成纤维细胞生长因子。

Synoviocytes were incubated with different concentration of methotrexate and total glucosides of paeony respectively or combined.

利用mtt比色法测定不同浓度的甲氨蝶呤和白芍总苷单独或联合作用于滑膜细胞，对滑膜细胞增殖的影响。

The results showed that about 3.18 x l0~7/ml of protoplasts were formed when the mycelia were incubated for 1 hour at 28 °C in TSB medium containing 2% glycin, and treated with 2 mg/ml lysozyme.

对东方拟无枝酸菌原生质体制备、再生及质粒DNA转化等条件进行了探索，考察了培养基组分、甘氨酸浓度、溶菌酶浓度等因素对原生质体的制备及其再生的影响。

Haemocyte membrane of Fenneropenaeus chinensis was purified and WSSV was labeled with DIG, the DIG-WSSV was incubated with MAb and the mixture was added to ELISA plate coated with membrane haemocyte, then the binding was detected with anti-DIG antibody.

提取中国对虾血细胞膜并对WSSV进行地高辛标记，将病毒和各单抗混合作用后吸附对虾血细胞膜，使用抗地高辛抗体检测结合到血细胞膜上的病毒。

In the SDBS experiment, no result could indicate that genomic DNA was damaged by SDBS, no matter L. japonicas was exposed to SDBS or DNA was incubated with SDBS. Fig 3, Tab 1, Ref 22

在十二烷基苯磺酸钠污染实验中，不论是作用于鲈鱼个体，还是直接污染其DNA，用所筛选出的6条引物扩增结果未发现对基因组DNA造成损伤，图3表1参22

Results: When incubated with different concentrations mitomycin for 24 hours, the body cells from 40μmol/L to 70μmol/L showed apoptosis under transmission electron microscope, chromatin condensation, even nuclear fragmentation, to form the apoptosis body can be seen.

体细胞经不同浓度的药物处理24h后，透射电镜下发现在40μmol/L～70μmol/L的各组细胞均有凋亡细胞，细胞核固缩、凝聚，甚至断裂，形成凋亡小体。

After the immunosensor was incubated with CEA solution at 37 ℃ for 30 min, the electron transfer access of CuL to CP was partly inhibited, which lead to a linear decrease of the catalytic efficiency to CP in pH 6.5 phosphate buffer solution, the decrease percentage of current had a linear relationship with the concentration of CEA between 0.1 and 80 ng/mL.

当该传感器在37 ℃下，含CEA的pH 6.5PBS溶液中温育30 min后，随着温育液中CEA浓度的增加，电极表面形成的免疫复合物也增加，导致CuL 对CP 的催化电流下降。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了