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The cocultures were incubated at 37 in 5% CO2 in air in DMEM-LG medium supplemented with 10% FBS.

已知与单核系定向分化有关的细胞因子有GM一cSF和M一cSF。

METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10^8/L were incubated in 12-well plate with a coverslip.

全骨髓法体外分离培养、纯化大鼠骨髓间充质干细胞，传至第4代时，按4×10^8 L^(-1)密度接种于置有盖玻片的12孔板内，制备细胞爬片。

METHODS: Normal human BMSCs were isolated and cultured by the whole bone marrow method. Cells of the third passage at 1×108/L were incubated on coverslip in a 6-well plate, and randomly divided into 3 groups: osteogenic induction group, which was added with primary medium and osteogenic inducer, supplemented with 10-8 mol/L dexamethasone, 10 mmol/L β-phosphoglycerol and 50 mg/L vitamin C; leptin osteogenic induction group was added with 50 nmol/l leptin in addition to osteogenic inducer. Cells in the blank control group were only treated with LG-DMEM containing 10% fetal bovine serum.

采用全骨髓法体外分离培养人骨髓间充质干细胞，传至第3代以1×108 L-1密度接种至预置盖玻片的6孔培养板中，设立3组：成骨诱导组添加原代培养液后，再加入含10-8 mol/L地塞米松、10 mmol/L β-磷酸甘油、50 mg/L维生素C的成骨诱导培养基；成骨+瘦素诱导组添加成骨诱导培养基后，再加入50 nmol/L瘦素；空白对照组仅加入含体积分数为10%胎牛血清的LG-DMEM培养液。

Cultured PC12 cells were incubated with Fluo 3-AM working solution (the final concentration of Fluo 3-AM is 5μmol/L) at 37℃for 45min, followed by 3 times washes with the culture medium. The cultured neurons grown on glass coverslips were placed in a cell chamber on the stage of an inverted microscope.

多种应激刺激通过细胞内信号转导途径使CREB第133位的丝氨酸磷酸化而激活，活化的CREB直接或间接激活相关基因的转录，进而表述某些蛋白分子以及某些神经营养因子等，进而调节神经元在应激性损伤后再生、存活及修复等。

Incubated with cytochalisin B for 4 hours, F-actin bunddles disrupted, confluent ECs monolayer has a complete detachment from the coverslips. So, there is a suggestion that the F-actin in ECs is essential for endothelial adherence under shear stress.

Northcrn杂交结果表明外源性纤维连接蛋白有促进EC肌动蛋白表达的作用，结合F-actin染色及FN受体染色结果，本文认为用纤维连接蛋白作为细胞种植的促粘附物质有助于内皮细胞粘附结构的形成。

BMSCs were isolated, depurated, cultivanted, and identified,then incubated with the concentration of 25μg Fe per milliliter at 37℃in 5% CO2. The labeled cells were stained by Prussian blue/trypan blue,and observed under fluorescent microscope.2. The labeled cells of different density (1×104/ml,5×104/ml,1×105/ml,5×105/ml,1×106 /ml,5×106/ml)were imaged by MRI with T1WI, T2WI and T2*WI sequences;and the same density (5×104/ml,1×105/ml)labeled cells were imaged by T2*WI sequences at different time.Then the signal intensities were measured and statistically analyzed.3. The model of rabbit renal ischemia-reperfusion injury was set up and treated. Then BMSCs(5×105)were injected into 16 recipient rabbits(1abeled cells in 10,unlabeled cells in 6)from ear vein.MR images of kidneys were obtained respectively at the time points of 0,1,3,5, 8 days after transplantation and before transplantation. MR imaging findings were analyzed,which were correlated with histological findings.

实验方法1分离、纯化、培养、鉴定兔BMSCs并以SPIO以25μg Fe/ml培养液浓度标记，对标记后不同时间的细胞行普鲁士蓝染色和台盼蓝拒染后显微镜观察。2将不同细胞浓度标记细胞管(1×104/ml、5×104/ml、1×105/ml、5×105/ml、1×106/ml、5×106/ml)，以不同扫描序列T1WI，T2WI，T2*WI(GRE进行MR成像，再选择相同细胞浓度组(5×104/ml、1×105/ml)进行不同时相MR成像，并测量信号强度，进行统计学分析。3缺血再灌注肾损伤模型建立和处理，然后将标记和未标记细胞(5×105个)经耳缘静脉移植入家兔体内(共16只：注入标记细胞者10只，注入未标记细胞者6只)，两组均于注射前、注射后第0、1、3、5、8天应用MRI对移植细胞进行活体示踪并与肾脏组织切片对照，然后对收集的信号强度进行统计学分析。

If tissue culture medium is incubated in dialyzers and then rat dorsal root ganglion cultures are exposed to this medium, characteristic changes occur.

EO残留量主要是指EO灭菌后留在物品和包装材料内的EO和它的两个副产品氯乙醇乙烷和乙二醇乙烷。

To test whether ethylene oxide retained in the dialyzers might be neurotoxic, tissue culture medium was incubated in the blood compartment of dialyzers.

如果组织培养基在透析器内孵育，然后将鼠的背根神经节（DRG培养物暴露于此培养基中，则DRG的特异性变化会发生。

There were also conspicuous accumulations of membrane bound esicles, dense bodies, myeloid figures, and areas of esicular in Schwann cells. These changes were seen with dialyzer exposed medium or with medium containing patient serum but not with controls or with medium incubated in plate dialyzers.

这些变化见于使用了暴露于透析器的培养基的DRG，或使用含有病人血清的培养基的DRG，但变化不发生于使用对照用组织培养基或者在平板透析膜中孵育的DRG。

Electron microscope findings were compared between DRG grown in control medium containing 10% calf serum, DRG incubated in the same medium exposed to the blood compartment of capillary flow or plate dialyzers and DRG grown in medium supplemented with 10% dialysis patient serum.

对下列基质中生长的DRG的电镜发现进行比较：即DRG在含有10%牛血清的对照基质中生长、然后在暴露于毛细流血隔室或平板过滤膜（ blood compartment of capillary flow or plate dialyzers）的相同基质中孵育；DRG生长于加有10%血透病人血清的组织培养基内。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了