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The effect of nitrogen source, initial pH, quantity of incubated in flask, culture temperature and time on producing chitinase of Pseudoalteromonas H7 were studied systematically in this paper.

系统地研究了氮源、起始pH、培养基装量、培养温度和时间等因素对交替假单胞菌H7产甲壳素酶的影响。

METHODS: The hUV-MSCs were isolated by the collagenase digestion method. At 1×108/L, hUV-MSCs were incubated in a 24-well culture plate, and then divided into two groups. These in the experimental group were treated with 150 g/L VEGF, whereas those in the control group were left intact.

采用胶原酶消化法分离人脐静脉间充质干细胞，以1×108 L-1的密度接种于24孔培养板，设立2组，实验组加入150 g/L血管内皮细胞生长因子，对照组不加入任何细胞因子。

After 72 hours incubation, the chondrocytes were digested into single cell suspension. Then they were incubated in the 96-well culture plate with the density of 1×10^8 L^(-1), 100 μL per well and six wells for per group.

白细胞介素1β加入量为5μg/L，培养72h将实验用细胞消化传代后制成单细胞悬液，以1×10^8L^的密度接种于96孔培养板，100μL/孔，每组6孔。

Methods A routine culture system of melanocytes was established, cells were irradiated with different dosages of UVB(30 and 90mJ/cm2 respectively), and then incubated with different concentrations of TMP for 72h. MTT method, NaOH assay and DOPA oxidization reaction were employed to determine the cell proliferation, melanin synthesis, tyrosinase activity of melanocytes.

常规分离培养正常人黑素细胞，分别用30mJ/cm2、90mJ/cm2的UVB照射细胞，然后加入100μg/ml、300μg/ml、600μg/ml的TMP孵育至72h，采用噻唑蓝法测定细胞增殖情况；多巴氧化法测定酪氨酸酶活性；NaOH裂解法测定黑素含量。

Main microorganisms of chilled mutton made in Tacheng area were incubated with selective agars.

采用选择性培养基从新疆塔城区冷却羊肉中分离和初步鉴定出乳杆菌2株、热死环丝菌2株、假单胞菌属和肠杆菌科各3株，葡萄球菌属和酵母菌各1株。

Maintypes of spoilage bacteria were incubated with selective agars to analyze the antisepticeffect of antimicrobials. The result showed: in the factor of antiseptic effect, nisin＞lysozyme＞lactic acid sodium＞chitosan＞tea polyphenol.

结果表明：5种保鲜剂的综合抑菌效果依次为Nisin＞溶菌酶＞乳酸钠＞壳聚糖＞茶多酚；Nisin和溶菌酶对革兰氏阳性的乳酸菌和热死环丝菌有很好的抑制作用，而对革兰氏阴性的假单胞菌属和肠杆菌科的抑制作用较差。

Cloacae E010 incubated in EKP could successfully inhibit disease development under low temperatures (8-16℃).

cloacae E010能够在低温之下仍然保持良好的产气防治病害发生的能力，罹病度皆在15%以下。

Methods: Myoblasts isolated from rat muscle samples were cultured and purified clonally in vitro. The 3rd passage cells were incubated with medium including basic fibroblast growth factor and dimethyl sulfoxid.

体外分离培养大鼠成肌细胞并克隆纯化，培养至第3代时加入含有碱性成纤维细胞生长因子和二甲基亚砜的培养液诱导分化，进行RT-PCR分析和免疫荧光细胞化学染色鉴定。

Results With BV coculture, the PC12 apoptosis was reduced to the least when incubated with injured PC12 media.

结果 小胶质细胞(BV2)和损伤PV12上清同时刺激MSC时，后者分泌的上清能显著降低受损PC12的凋亡数目。

The sperm were incubated in the medium with 20% FF for 3 hours after cocultured with LIOEC for 3 and 6 hours.

检测9hr培养时间内精子的存活率。3、精子在与HOEC共培养后3hr和6hr，加入20％FF作用3hr，检测其AR率。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了