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The cells were incubated with Alexa 488 conjugated Staphylococcus aureus bioparticles opsonized with rabbit IgG for 1h, followed by fixation and incubation with Alexa 633 conjugated goat anti-rabbit IgG to distinguish ingested from extracellular bioparticles.

将兔抗IgG 作用于Alexa 488金黄色葡萄球菌的活性颗粒，后与NTM细胞孵育1小时，再用Alexa 633羊抗兔IgG定位，以区别所吞噬的位于细胞外的活性颗粒。

An actively respiring bacterial culture is briefly incubated with [l-14C]glucose, and the glycolytic and citric acid cycle intermediates are isolated.

一种活跃呼吸的细菌暂时用[1-14C]葡萄糖来孵化培养，糖分解和三羧酸循环的中间产物被分离出来。

The mechanical contraction of circle muscularis strips, the contractile response of dispersed smooth muscle cells, the change of 〓 stimulated by sennoside with different concentration and that stimulated by Ach after incubated with sennoside were evaluated. The translocation of RhoA by sennoside were measured by laser confocal scanting microscopy too.

检测不同浓度的番泻甙对小肠环行肌条和游离环行平滑肌细胞的收缩作用及对〓的影响，然后测定番泻甙对Ach刺激的小肠环行肌条、游离环行平滑肌细胞收缩反应和细胞内〓变化的影响作用，并通过免疫荧光实验检测番泻甙对RhoA的活化和空间转位的影响。

To determine whether heparin could decrease VEGF concentration by sequestering VEGF in the endothelium, a fixed dose of recombinant VEGF was incubated for 40 minutes with EA.hy926 endothelial cells in vitro.

我们通过分离内皮细胞中的VEGF浓度来确定肝素是否能够降低VEGF浓度，剂量确定的重组VEGF与EA.hy926内皮细胞在体外培养40分钟。

For quantitatie real-time PCR of DR4 and DR5 transcripts, triplicated samples containing 9 l of cDNA with 10 l of Taqman Uniersal PCR Master Mix (Applied Biosystems, Foster City, CA) and 1 l of 20xAssays-on-demand Gene Expression Product (DR4; Hs 00269492_m1, DR5; Hs 00366272_m1, Applied Biosystems) were pre-incubated at 50oC for 2 minutes and subsequently at 95oC for 10 minutes.

对于进行定量实时PCR的DR4 和 DR5转录产物，使用10L 美国应用生物系统公司生产的 Taqman Uniersal PCR Master Mix试剂，1L的20xAssays-on-demand Gene Expression Product (美国应用生物系统公司生产的DR4； Hs 00269492_m1， DR5； Hs 00366272_m1)，先将其在50度下孵化2分钟，然后在95度下孵化10分钟，用这两种试剂将含有9L cDNA的样品扩增两倍。

The reaction between antigen and antibody was carried out by one step balance method and incubated in 4℃ for 24 hours, then separated bond and free antigen by PR reagent.

用氯氨T法制备125I标记IL-6，经Sephadex G-25纯化，抗原抗体反应采用平衡一步法，4℃温育24 h后经PR试剂分离结合和游离的标记抗原。

Histochemical assays of GUS gene showed those resistant callus and roots from transgenic plants cultured under light were no blue-stained with X-gluc, but callus and roots after 24 h treatment of darknees and then incubated with X-gluc could present blue. Fluorimetric enzyme quantitative analyses indicated GUS expression in transgenic plants was organ specificity.

转基因抗性愈伤和转基因植株根的组织化学GUS活性检测表明，光照培养下的抗性愈伤和转基因植株根不能使X-gluc显色，而暗处理24 h后的抗性愈伤和定植后转基因植株的根能使X-gluc显色。

Summarization of the literatures in this research field are made comprehensively. The experiments are made as follows: H〓S and CH〓SH metabolizing micro-organisms are incubated and enriched in the bottles, into which quantitative inorganic nutrient salt and phosphate buffer are added.

研究以全面的文献综述为基础；微生物驯化培养采用液相硫化物加富培养筛选的方法；微生物包埋方案比较了多种包埋主剂、助剂和交联条件；固定化微生物颗粒填充滴滤床实验探索了驯化液pH值控制、循环液流量、处理负荷等因素对生物脱臭的影响。

They incubated the virus in a cobalt chloride solution so that cobalt oxide crystals mineralized uniformly along its length.

研究者调控后病毒的 M13 基因，使得病毒外层或者被膜能附着某些金属离子，他们将病毒孵化在氯化钴溶液中，氧化钴晶体沿着病毒外层均匀地矿化出来。

Subsequently, BMSCs were incubated in serum-free L-DMEM, and placed in a plastic culture flask at 1.0×108/L.

按1.0×108 L-1的密度接种到一次性塑料培养瓶中进行培养。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了