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METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

To characterize the isotype of human xenoreactive nat ural antibodies against porcine pancrease islet cells, cyostat sections of the p ancrease were incubated with the sera from 18 cases of IDDM or 20 human controls respectively, further with FITC-conjugated goat antihuman IgG or IgM secondary antibodies. IgG was found to bind to pancrease islet cells in sera from 8 cases of IDDM and 8 human controls, while IgM in 4 IDDM and 6 human controls.

为检测人抗猪胰岛细胞天然抗体的同种型，采取18份胰岛素依赖型糖尿病患者血清及20份正常人血清分别与杂种猪胰腺组织的冰冻切片孵化后，再用免疫荧光标记的羊抗人IgG、IgM单克隆抗体染色。8份IDDM血清和8份正常人血清中含有直接针对猪胰岛细胞的IgG型抗体；4份IDDM血清和6份正常人血清中含有IgM 型抗猪胰岛细胞抗体。

Then the expression of ICAM-1 incubated with or without areca nut extract in the presence of 10％ fetal calf serum for 48 h at 37 ℃ in 5％ CO2 and air were measured by using enzyme-linked immunosorbent assay.

分别从正常及 OSF患者的口腔黏膜中培养出 FB，向培养基中加入不同浓度的 ANE培养 48 h后，用细胞酶联免疫方法检测 FB表达的 ICAM-1水平。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

The increased amount of non--exchangeable NH4^＋-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^＋-N （P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively）. NH4^＋-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^＋-N increased significantly when added （NH4）2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^＋-N from （NH4）2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^＋-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d （P=0.0434）, but not significantly at 40 d and 60d （P=0.7378 and 0.5375）. The increased amount of non-exchangeable NH4^＋-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^＋-N, the increased amount of non-exchangeable NH4^＋-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^＋-N的增加量均表现为以土垫旱耕人为土最大，其次是黄土正常新成土，简育干润均腐土和干湿砂质新成土较小；添加有机物料极显著影响培养期间的非交换性NH4^＋-N增加量（培养20d、40d和60d时P分别为0.0002，0.004和0.0003），表现为紫花苜蓿和长芒草土壤非交换性NH4^＋-N的增加量均极显著高于不添加有机物料的对照土壤；在培养20d、40d和60d时，加（NH4）2SO4土样非交换性NH4^＋-N的增加量显著大于不加（NH4）2SO4土样（户分别为0.0037，0.0033和0.0027），这是土壤对（NH4）2SO4中NH4^＋-N固定的必然结果；不同植被类型土壤培养20d时的非交换性NH4^＋-N增加量差异显著（P=0.0434），培养40d和60d时差异不显著（p分别为0.7378和0.5375）；长期秸秆和氮肥配施土壤非交换性NH4^＋-N增加量大于不施肥对照土壤，但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^＋-N含量均呈极显著正相关；而非交换性NH4^＋-N的增加量与粘粒无相关性，但与全氛和有机质呈显著正相关。

METHODS: Femur and tibia were obtained from Wistar rats for primary culture of BMSCs. MSCs at passage 15-20 were firstly incubated in preinduction medium, for 24 hours, and then incubated in induction medium containing α-MEM, 2% dimethyl sulfoxide and 200 μmol/L butylated hydroxyanisole.

取Wistar大鼠股骨和胫骨，进行骨髓间充质干细胞原代培养，传至第15~20代融合状态的间充质干细胞置于预诱导培养基中培养24 h后，换用诱导培养基。

The affect degree of different performance factors of incubated business on the performance of incubated business is differently.

孵化企业的不同绩效影响因素与孵化企业的不同绩效指标之间具有不同程度的相关关系。

Comparison of gas-producing bacteria incubated in EKP or CTP and chemical treatments showed that gas-producing bacteria incubated in EKP or CTP was much more effective in controlling orange green mold than chemical treatments.

利用产气细菌制剂所产生之气体与腐绝及免赖得等化学药剂比较防治柳橙绿霉病的效果，发现E010与CHT2401产气防治病害发生的效果远优於化学药剂。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了