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Methods: human neutrophil was pre-incubated with Safflor yellow of different concentration, then was stimulated by platelet-activating factor with the existence of cytochalasin B.

通过细胞学的方法，在细胞松弛素B存在下，人的中性粒细胞悬液先与不同浓度的红花黄色素温育，然后以PAF刺激，以PMN悬液为空白对照。

Methods Porcine coronary SMCs were incubated with serum-free medium containing different concentrations of simvastatin. Infiltration of 3H-TdR into SMC DNA was measured as an index of cell proliferation. Simvastatin's effect on migration was tested with Sarkar's technique.

采用体外猪冠状动脉SMC培养技术，以3H-TdR的参入量表示冠状动脉的SMC DNA合成情况，应用划线方法测定冠状动脉SMC的迁移距离，观察辛伐他汀对OX-LDL诱导的冠状动脉SMC增殖和迁移的影响。

After changing medium, BMSCs were incubated in serum-free DMEM containing BRA and dimethyl sulphoxide for 5 hours to 6 days. MAIN OUTCOME MEASURES: Cell transfection was observed with a fluorescence microscope.

主要观察指标：荧光显微镜观察细胞转染情况，免疫细胞化学染色检测转染细胞诱导后神经元表面抗原的表达。

A colloidally stable suspension of nanoparticles was incubated on sheep synovial cells in vitro for 3, 24, 72, and 120 hours.

将纳米颗粒胶质的稳定悬浮液与羊滑膜细胞体外培养3、24、72和120小时。

Methods The cells of LLC-PK1,Hela,Caki-1,T24 and fibroblast were incubated on 96-w ell plate with addition of diffe-rent concentration of cystic fluid of ACDK and t hat of simple renal cyst. After 24 hours for reaction, MTT method was used to de termine and compare the proliferative activity of 5 cell lines.

将LLC-PK1， Hela， T24， Caki-1和成纤维细胞接种于96孔培养板中，经过24h培养，加入不同浓度的ACDK和单纯肾囊肿的囊内液作用24h，用MTT法测定培养细胞的增殖活性，比较两者之间对细胞增殖的抑制作用。

The results showed that NRA was higher in the light than in darkness;NRA increased when thalli were incubated in NO_3~——N,and it enhanced faster in the light than in darkness.

另外，对于N加富→N饥饿的处理，在光照下NO3--N加富和NH4+-N与NO3--N共同加富的NR都能在N饥饿处理后的一段时间内维持较高的活性。

During the fermentational coagulation of the pasteurised cow milk incubated at ambient temperature for making yaourt, the milk motive viscosity firstly went gradually up until to the highest point, and then it came down comparing to the gradual rising titrable acidity and the gradual descending pH value.

全脂牛奶在室温发酵制作酸乳过程中，其酸度随着发酵时间的延续和微生物添加量的增加而上升，pH值则逐渐降低；而酸乳粘度则随着发酵时间的延续上升达到最高点开始下降。当微生物添加量为 4 %～ 5%，发酵 1 2h时，酸乳凝乳良好，口感适宜，活性乳酸菌总数达到 1 0 7数量级

Reaction was stopped by adding 300 μl of 10% trichloroacetic acid and samples were incubated on ice for 30 min.

加入300 μl 10%的三氯乙酰酸来终止反应，然后将样品在冰中保温30分钟。

1MM GTP was mixed with tubulin (2mg/ml) and varying concentrations of sodium selenite. The mixture was incubated at 37℃ for 1hr. MTs were separated from unassembled tubulin by centrifugation. Both supernatants and pellets were subjected to SDS-PAGE.

其中一组实验是在相同的微管蛋白体系中加入不同浓度的亚硒酸钠，然后加入GTP，37℃聚合，离心，沉淀为聚合的微管，而上清液为未聚合的微管蛋白。

In the segments of ileum incubated with U50488H(10 nmol*L-1) for 1 h, the sensitivity for U50488H was reduced, the concentration-response curve was shift to right, but the sensitivities for OMF, NM and DADLE were unchange.

在U50488H(10 nmol*L－1)中温育1 h的回肠，对U50488H的敏感性下降，浓度反应曲线右移，但对OMF，NM，DADLE的敏感性不变。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了