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The blots were probed with anti-Cdx-2, anti-JNK (Santa Cruz Biotechnology, Santa Cruz, California), anti-ERK, anti-phospho -ERK, antip38MAPK,anti-p-p38MAPK, anti-p-JNK (Cell Signaling, Beverly, Massachusetts) and anti-actin (Amersham Life Sciences,Piscataway, New Jersey) antibodies, and then incubated with appropriate species specific secondary antibodies labeled with horseradish peroxidase.

Blots分别与抗CDX-2抗体，抗JNK抗体（圣克鲁斯生物技术，圣克鲁斯，加州），抗ERK抗体，抗phospho -ERK抗体，抗p38MAPK抗体，抗p-p38MAPK抗体，抗p-JNK抗体（Cell Signaling， Beverly，美国马萨诸塞州）和抗actin抗体（ Amersham生命科学，皮斯卡塔韦，新泽西州）作用，然后在与辣根过氧化物酶标记的第二抗体孵育。

Ca 2+ at 1 64 mmol/L markedly increased ethanol tolerance of a self_flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20% ethanol at 30℃, no viability remained for the control whereas 50 0% remained for the cells both grown and incubated with ethanol in Ca 2+_added medium.

生长阶段和冲击阶段均添加 1 6 4mmol LCa2 +能显著提高自絮凝颗粒酵母于 30℃在 2 0 %酒精冲击下的存活率，经过 9h冲击，对照组的存活率为 0 ，而添加Ca2 +试验组的存活率为 5 0 0 %，表明添加适当浓度的Ca2 +能显著提高菌体的耐酒精能力。

Rapid dehydration is the worst.4. Effect of preculture Beads were encapsulated and incubated in culture medium containing 2% PEG and 5% PVP respectively for 90 min before dehydration.

预培养的影响将胶球在分别含有2%聚乙二醇和5%聚乙烯吡咯烷酮的培养基中预培养90min后脱水。

Eggs or nauplii I, hatched under optimal conditions (30°C, 30 ppt), were incubated under 15 different combinations of three temperatures (20°C, 25°C, and 30°C) and five salinities (15 ppt, 20 ppt, 25 ppt, 30 ppt, and 35 ppt) until reaching protozoea I stage.

中文摘要：本研究在探讨温盐对中型毛虾无节幼体变态之影响。在三种温度（20、25及30℃）和五种盐度（15、20、25、30及35ppt）的十五种组合中，观察在最适当状况下（30℃、 30ppt）孵化的卵或无节幼体变态成蚤状幼体第一期。

The results showed that inoculum, initial pH, incubated temperature and additive had great effects on Pythium.

结果表明，接种量、起始pH值、温度、添加物对腐霉菌丝形态影响较大，接种量3%~10%，培养温度25~30℃，起始pH4及pH6.5~9，添加植物油能使菌丝保持直径小于5mm的分散小球状态。

Pythium grows to disperse while inoculum 3%~10%,incubated temperature 25~30℃, initial pH 4 and pH 6.5~9, added Soybean oil.

同时，起始pH值及培养温度显著影响EPA含量。

These osteoclasts had low resorptive activity when incubated on bone slices.

将这些破骨细胞在骨片上进行培养时，其再吸收活性较低。

The PHA stimulated CBMC which was then incubated with rIL-2 began to express IL-4 mRNA after restimulation of PMA plus iono.

将PHA刺激后的CBMC与rIL-2一起孵育，再用PMA+IONO刺激，CBMC始表达IL-4 mRNA。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

In the first experiment, CL cells were incubated with 〓-tyrosine at 24℃ for 45 min. The binding of 〓-ty- rosine to the CL cells reached saturation at 12 μM of 〓-tyrosine con- centration.

将黄体细胞与〓-酪氨酸一起孵育（24℃，45min）后，〓-酪氨酸能与黄体细胞特异结合，〓-酪氨酸浓度为12.5μM时达到饱和，在4℃孵育10min的条件下仍有〓-酪氨酸的结合。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了