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Methods: Recombinant human PI3K p110β subunit was constructed by gene engineering, and then incubated with phosphatidylinositol-4, 5-bisphosphate, 32P-labeled ATP and myricetin, quercetin or kaempferol.

通过基因工程技术的方法获得PI3K p110β催化亚基。

METHODS: Cultured THP-1 monocytes were induced to macrophages by 0.1 μmol/L phorbol myristate acetate, and the differentiated THP-1 macrophages were incubated with 200 μmol/L Hcy for 24, 48 and 72 hours respectively. Then cell supernatant and lysate were collected as condition medium.

在由0.1 μmol/L 佛波脂诱导分化的THP-1单核细胞中加入200 μmol/L 的高同型半胱氨酸培养24，48，72 h，将各时间点细胞上清液及裂解液作为条件培养基，加入经地塞米松及维生素D3作用6 d 后的培养瓶中继续培养24 h。

In the presence of an opioid analog,DAMA10(-13～10(-10)mol/L,human granulocytes and Mytilus edulis immunocytes were incubated with or without ligands at 37℃ for 10 minutes and at 23℃ for 20 minutes, respectively.

将人粒细胞和Mytilusedulis免疫细胞分别与不同浓度的类鸦片肽DAMA（10（－13）～10（－10）mol／L）或δ拮抗剂在37和23℃保温10～20min，用计算机显微图像分析法分析两种细胞的活化情况。

Moreover, all of the incubated cells, including rat oligodenderocyte, human brain cell of oligodendroglioma and non-oligodendroglioma, could secret β-tp.

培养的大鼠胶质细胞随细胞数增加而β-tp水平增高。

To study levels of beta-trace protein in the cerebrospinal fluid with oligodendroglioma patients and in the mediums of incubated rat oligodenderocyte.

蒋雨平 ，蔡振林，任惠民目的：探索神经胶质细胞及人脑胶质瘤细胞是否能分泌β-tp。

METHODS: Osteoblastic cells from rats were incubated in the medium supplemented wiht rhgh at different doses (10, 200 μg/L), and then the changes of cytoskeleton and cell cycle were detected.

用浓度为10和200 μg/L的重组人生长激素加入大鼠OB培养体系，观察对大鼠OB的细胞周期及细胞骨架的影响。

BMSCs were purified by the differential adherence. At the third passage, BMSCs were incubated in osteogenous, adipogenic and chondrogenic medium for 14 days.

取生长状态良好的第3代骨髓间充质干细胞，分别加入成骨、成脂、成软骨诱导培养基，培养14 d。

When tHe female have taken enough blood and dropped from donkey's body,they wereput in incubator to oviposit and incubated at 28℃ and 90%RH.

将实验室培育的&清洁&草原革蜱饥饿成虫，饲喂在人工感染驽巴贝斯虫的驴体上，俟雌虫饱血脱落后，置28℃、相对湿度约90％的培育箱中产卵孵化

Vibrio cholerae adherence to and colonization in the small intestine are essential step in the pathogenesis of cholera. At least one type of pili, known as TcpA, is required for the colonization process. This paper reports the electron microscopic evidence showing that V. cholerae 01 and 0139 serogroups can express at least 4 types of pili when incubated at 28℃ on AKI agar or CFA (pH6.8). Among the four types, three can be expressed by classical and El Tor strains of Vibrio cholerae 01: TcpA pili are peritrichous,being 7-8nm in diameter, 5-10μm in length, and form bundles of parallel undulating filaments of 0.5-0.8 μm wide and 10-15μm long; Type B are 3-4nm in diameter, up to 2μm long, and of wavy morphology; Type C are 7-8nm wide and 0.5-0.8μm long.

本文通过电子显微镜对01群霍乱弧菌古典生物型和El Tor生物型以及0139群霍乱弧菌研究发现，在培养基AKI和CFA(pH6.8)28℃培养条件下，01群EVC和CVC均可产生TcpA，B型和C型菌毛；0139群霍乱弧菌可产生TcpA和B型菌毛，与01群一致。

By using red blood cell broken-up solution,proteinase K and RNase,total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species,Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.

本实验以河南董寨自然保护区白冠长尾雉和环颈雉已孵化的卵壳为材料，利用红细胞破碎液、蛋白酶K及RNA酶等试剂，对卵壳膜内的总DNA进行了提取，建立了一种提取高质量DNA的新方法。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。