查询词典 incubated

Result:(1)Tau prevented neurons against Mn toxicity of low and middle concentrations. The ideal conditions can reach upon 4. 5 mM Tau added just, nearly no apoptosis cells could be detected.(2)The percentages of apoptosis intogether were lower than those in Mn just , being concentration-dependent relations with Taurine levels.(3)Mn induced DNA damage of neurons with dose-depedent, The higher of Mn concentration, the lengther of the comet tail and the higher percent comet-like cells. Tau incubated could weaken significantly the lesions of Mn towards DNA on the degree.

结果：(1)牛磺酸对中低剂量锰的毒性有一定的拮抗作用，单独加入牛磺酸效果最好剂量是4.5mM，视野下几乎不见凋亡细胞；(2)牛磺酸干预后培养神经细胞凋亡率低于相同剂量的单纯锰培养细胞组，且与牛磺酸浓度呈剂量依赖性；(3)锰引起培养神经细胞DNA损伤，表现在彗星尾长增加，彗星样细胞百分率增加；加入牛磺酸干预后，可明显改善锰造成的培养神经细胞DNA损伤。

Methods: Rats lipocyte of eight weeks swimming rats and control rats were incubated by RBP4. Glucose uptake, protein expression and phosphorylation of IR and IRS-1 in lipocyte were measured.

RBP4 孵育8 周游泳运动组和安静对照组大鼠（8 周龄SD）脂肪细胞，检测脂肪细胞葡萄糖摄取、IR 和IRS- 1 蛋白表达和磷酸化。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Methods VECs were isolated from human umbilical vein and incubated with two kinds of dicarbonyl compounds,3-deoxyglucosone (3-DG) or methylglyoxal.

方法从人脐静脉分离VEC并与不同浓度的二羰基化合物共同培养。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

Results As incubated with Hcy,monocytic cells can be induced to express TF,which is upregulated in dose-dependent pattern.

结果 随着Hcy浓度的增加，TF在乳腺癌患者外周血单核细胞的表达水平逐步增高，并呈剂量依赖性。

Simple modified differential adherence: monoplast suspension at 8 000/cm2 was incubated in uncoated 25 cm2 flask twice for 12 h +24 h subsequently, the cell suspension was drawn off.

单纯改良差速贴壁：将单细胞混悬液以8 000个/cm2密度种植于无包被的25 cm2培养瓶中。

METHODS: Rat cerebral cortex was obtained sterilely, made into monoplast suspension by the mechanical separation, and then incubated in the serum-free MEM/F12 medium, supplemented with B27 neurotrophic additive for 1 week. Subsequently, cells were passaged.

无菌条件下取胎鼠大脑皮质，剪碎后机械分离法制作单细胞悬液，加入含B27神经营养添加剂的无血清MEM/F12培养基进行培养，1周后传代。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Methods The flavonoids from extracts of morus folium in different concenration (0.001,0.01,0.1 g/L) were added to a glycosylation system in vitro,and incubated for 1,4,7,14,21 ,28 days respectively,then the early glycasylation products were measured.

方法从桑叶中提取不同浓度(0 0 0 1，0 0 1，0 1g/L)总黄酮加入体外白蛋白糖基化体系中，分别孵育 1，4 ，7，14 ，2 1，2 8天，测定早期糖化白蛋白产物的浓度。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了