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METHODS: PVA filature was used to weave fascicular scaffolds. NIH-3T3 cell line and human ACL cells were in vitro incubated, amplified, and then implanted on the PVA/COL scaffolds.

用聚乙烯醇纺丝编织成条束状支架材料，用NIH-3T3细胞株和人前交叉韧带细胞体外培养、扩增后，分别种植到聚乙烯醇和经Ⅰ型胶原胶修饰过的聚乙烯醇纺丝纤维编织支架材料上，立体培养。

Blood samples were obtained on 0,2,4,6 and 8 days after admission. Dynamic changes of immunological parameters including serum IgA,IgG,IgM,complements (C3,C4) levels were determined in each group at various intervals following trauma. Phagocytosis of monocytes was also measured by use of phagotest kits after blood cells were incubated with fluorescin isothiocyanate-labeled E.

分别在入院时及入院后2、4、6和8 d留取外周静脉血，检测血清免疫球蛋白(IgA、IgG、IgM)与补体(C3、C4)水平的动态变化，全血细胞与用异硫氰酸荧光素标记的大肠杆菌共孵育后检测单核细胞噬菌能力。

To examine the effect of photoperiods on sexual reproduction of sea weed Hizikia fusiformis Okamura, receptacles were collected and incubated in three different light periods.

对羊栖菜的生殖托在不同光照周期条件下进行离体培养，观察卵细胞的释放与受精情况，以及受精卵的发育情况，以探讨光照周期对其有性繁殖过程的影响。

Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10-8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×107 L-1接种后各分为2组：诱导组加入含10-8 mol/L地塞米松、10 mol/L β-甘油磷酸、50 mg/L维生素C的成骨诱导培养基1.5 mL，未诱导组不加入成骨诱导培养基。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

In order to study the influence of nitric oxide and copper ion on marine algae growth,Skeletonema costatum and Gymnodinium sp.were incubated when adding different concentrations of NO and copper with different addition methods,and the preliminary functional mechanism was discussed.

为研究一氧化氮与金属铜对海洋微藻生长的影响，选用中肋骨条藻和裸甲藻(Gymnodinium sp。)进行了不同NO和Cu浓度、不同添加方式的室内培养实验，初步探讨了它们的作用机制。结果表明：一次性或每天两次加入不同浓度NO(10-5~10-10mol/L)，对微藻的生长表现出不同程度的促进或抑制作用，呈&两面性&。

At the fifth passage, two cell strains were made into cell suspension at l×10^9L^(-1). 0.5 mL was subcutaneously incubated at the inguina of each rat for 8 weeks. Amplified cells were cultured in PGC culture medium and non-adherence culture flask till idiosome formed, adhered and differeotiated.

体内实验取连续传5代的2个细胞株，分别制备浓度为l×10^9L^(-1)的细胞悬液，于每只小鼠两侧腹股沟处皮下各接种0.5 mL，培养8周体外分化采用无私附特性的培养瓶和除去白血病抑制因子的原始生殖细胞培养液，以脱饲养层法悬浮培养扩增的细胞，直到拟胚体形成及贴壁分化。

Methods Keratinocytes were isolated from the foreskins of children, and subjected to primary culture. The cultured keratinocytes were incubated with anti- keratin 16 antibody, or with isotype antibody as control, for various durations.

将抗角蛋白16抗体加入培养的角质形成细胞中，以同种型抗体作对照，研究外皮蛋白和Toll样受体2和4 mRNA表达。

D limonene was distributed among the culture flasks (200 mg/L). The flasks were incubated at 28oC on a rotary shaker (r/min=120) for 5 days. On completion, the culture broth were successively extracted with EtOAc. The extract was quantitatively measured by gas chromatography mass spectrometry analysis, The results showed that biocatalytic reactions were comprised of oxidation, including hydroxylation and ketonization which occurred at different positions of the D limonene, as well as hydrogenation, hydrolyzation, esterification and cyclic cleavage. These bacteria strains possess the distinctive biocatalytic capacities towards the D limonene. A series of terpenoids, including sesqui , di and tri terpernoids were also observed,which was accounted for D limonene had influence on the metabolites of marine bacteria.

以2216E为培养基，添加200mg/L的柠檬烯，在28℃，以120 r/min摇瓶培养5 d，用乙酸乙酯提取培养液，经GC MS分析其转化产物，结果显示，这些细菌能在柠檬烯的不同位置进行羟基化、羰基化等，并伴随有还原、水解、酯化、开环等反应，但转化能力和转化程度不同；在产物中，还检测到系列结构不同的其它萜类：包括倍半萜、二萜以及三萜等，这些萜类化合物的产生跟柠檬烯的加入有关，说明柠檬烯能影响细菌代谢产物的产生。

Its peak of laccase production came out after incubated 4 days.

对产酶条件的初步研究结果表明，WS1-2菌株的产酶高峰期出现在接种培养后的第4d。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了