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METHODS: 10 mL bone marrow was extracted from dog posterior superior iliac spine, heparin anticoagulation, diluted using Hanks juice, treated with 1.077 g/mL Ficoll solution 3 mL, and centrifuged at 2 000 r/min for 20 minutes. Karyocytes were absorbed to form white cloudlike layering interface, and then centrifuged twice using DMEM supplemented with fetal bovine serum, incubated at 12×104/cm2 at 37 ℃ in a 5% CO2 incubator.

于犬髂后上棘抽取骨髓液10 mL，肝素抗凝，Hanks液稀释后，加入1.077 g/mL Ficoll液3 mL，2 000 r/min离心20 min，吸取有核细胞形成白色云雾状的分层界面，用含胎牛血清的DMEM离心2遍，按12×104/cm2密度接种，于37 ℃、体积分数为5%的CO2培养箱内培养。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

Methods AMMC were incubated with different concentrations of aqueous extracts of Cuscuta chinensis Lain and 8-MOP, then activity of tyrosinase, melanin content and rate of proliferation of AMMC were tested respectively.

方法以8-甲氧补骨脂素（8-MOP）作为阳性对照，选择系列浓度的中药菟丝子水提取物和8-MOP作用于体外培养的AMMC，然后测定酪氨酸酶活性、黑素含量和细胞增殖率的变化。

This indicates that both the reductive dissolution of ferrihydrite and the reductive desorption of As occurred in the batches incubated with arsenicresistant denitrificator and Asadsorbing ferrihydrite.

这说明，体系中水铁矿的还原性溶解和As的还原性解吸已经发生。

And dissimilatory reduction capability of crystal Ferric phosphate is also investigated when using different organic acid as substrates. Water-extracted soil solutions of SiChuan, JiangXi, JiLin and HuNan were incubated under anoxic atmosphere.

在水稻土浸提液为接种液的富集培养中添加不同浓度的磷酸缓冲液作为磷源，当磷酸盐浓度大于30 mmol/L时，铁还原反应的速率和Fe的产生量相对较低，甚至有抑制作用。

In hypoxia/reoxygenation model(Na2S2O4), cell survival was enhanced and LDH in culture medium was reduced(P.05, P.01) after treated with 60, 30μgmL^(-1) Sanguis Draconis flavones. Enhanced cell survival and reduced LDH in culture medium(P.05, P.01) also observed in cytotoxicity model induced by H2O2 after incubated with 60, 30, 15μgmL^(-1) Sanguis Draconis flavones.

在缺氧／复氧(Na2S2O4)模型中，60、30μgmL^(-1)龙血竭总黄酮能显著降低培养液中的LDH浓度，增强细胞活力(P.05， P.01)。60、30、15μgmL^(-1)龙血竭总黄酮能抑制H2O2引起的损伤，降低培养液中LDH的浓度，增强受损细胞活力(P.05， P.01)。

Embryonated eggs are edible eggs during incubated period. It is gift of food, nutrition and curative by its fresh taste and great efficacy.

鸡胚蛋就是受精鸡蛋经特殊孵化工艺，胚胎不断生长发育，可食用的具有生命力的鸡蛋。

Neurons treated with 20-mmol/L-theophylline did not show 180bp ladder characteristic of endonuclear activation on its DNA gel electrophoresis. The ［cAMP］i of the neurons rose after being incubated together with different concentration theophylline (5-80 mmol/L) for 15 minutes in CO2 incubator and the prometed range relied on the dose of theophylline.

100％，量效关系有高度显著性(P.01)，EC50=2.97mmol/L；经茶碱(20mmol/L)处理的细胞，其DNA凝胶电泳带没有&梯子&状分布；与不同浓度茶碱（5-80 mmol/L）在CO2培养箱孵育15 min，小脑颗粒神经元内cAMP均升高，且是剂量依赖性的。

Methods: The HUVEC was incubated with exogenous AGEs for 12 h, and ergamine and catalase were used as control. The activity, monolayer permeability, apoptosis rate, biochemical indexes and morphous changes were detected in HUVEC.

外源性AGEs与HUVEC共孵12h，以组胺和过氧化氢酶为对照，检测内皮细胞的活性、单层通透性、凋亡率及细胞培养液生化指标，并且观察内皮细胞形态学改变。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了