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Virus was also cultured in allantoic cavity of chicken embryo with statistically significant HA titer at 1:6.8 as compared with virus control group (P.01) incubated 24 hours after injected with MEK first then injected with IBV.

在鸡胚尿囊腔中，先注入MEK孵育24 h后，再注入B型流感病毒的鸡胚也培养出病毒，HA滴度为1：6.8，与病毒对照组比较P.01，有统计学意义。

Methods Radio-immunological and fluorescence methods were used to determine the amount of insulin secretion as well as content of intracellular Ca++ and cAMP by the rat islet cells incubated with amylin in different concentrations.

用放免和荧光方法分别检测经不同浓度胰淀素温育的大鼠胰岛细胞的胰岛素分泌量、细胞内Ca++及cAMP含量。

The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

该转化子在YPDS平板30℃培养48小时形成清晰可见的水解淀粉圈。48hrs发酵液可获得0.74-0.78u/ml产酶活力及高的生物量。

The glucoamylase I gene was inserted into the pAC1 plasmid,and a expression plasmid was constructed,then it was used to transform the yeast strain, S.cerevisiae AS2.1364,without auxtrophic marker,The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃ for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

这说明酵母转化子不仅表达了该酶，且有效地分泌到胞外，从而水解培养基中的淀粉，进一步测定酵母转化子48hrs发酵液的葡萄糖淀粉酶活力为8.4u/ml，培养基中的淀粉已完全水解，其生物产量较高。

This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells. The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method.

为研究苯甲醇对海藻糖负载红细胞的影响，在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时，用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率，用硫酸蒽酮法检测红细胞内海藻糖浓度水平。

In the forth chapter, the mechanism of pyrimidine benzylamine compounds is studied by the conventional bioassay and biochemical methods based on probe compound 104-4. The results presented here indicated that the addition of 5 mg/L of 3 branched-amino acids to sorghum seedling cultures incubated in the presence of 104-4 completely alleviated herbicide-induced stem growth inhibition, but each of Val, Ile and Leu single is only partly effective in reversing 104-4-induced growth inhibition at the same concentrations.

通过先导结构优化策略的实施，筛选出15个高除草活性的4，6-二甲氧基-2-取代嘧啶苄胺类候选化合物，在此基础上，对一些候选化合物进行作物安全性评价试验，实验结果提示化合物103-1和化合物105-3在低剂量下对某些作物有良好的选择性，可作为优选化合物进入田间小区试验。

Methods Hep G2 cells were treated with Aroclor1254,DDT and Toxaphene respectively for 24 hours,and further incubated in the medium supplemented with cytochalasin B(3?μg/ml)for 24 hours.Micronucleiwere scored in 1?000 binucleated Hep G2 cells.

方法人肝肿瘤细胞Hep G2经Aroclor1254、毒杀芬和滴滴涕染毒24 h，继续在补充细胞松弛素B(3μg/ml)的培养液中培养24 h后，计数1 000个双核细胞中的微核。

Newborn rat calvaria osteoblastic cells were incubated with TNF-α(20、30、40ng/ml) for 24 and 48h.

用TNF-α 20、30、40ng／ml的量刺激成骨细胞，观察24、48小时，测定细胞培养液中亚硝酸根含量。

Methods Monocytes in peripheral blood were obtained from 12 patients with acute myocardial infarction, 16 unstable angina pectoris, 12 stable angina pectoris and 8 normals by ficoll density gradient centrifugalization. The cells were incubated for 48 hours to induce them to differentiate into macrophages by adding phorbolester 12-myristate 13-acetate. The levels of MMP-9 and TF in the supernatant were measured by ELISA.

密度梯度离心法提取12名急性心肌梗死、16名不稳定心绞痛、12名稳定型心绞痛患者和8名冠脉正常者的单核细胞，用佛波酯诱导其分化为巨噬细胞，ELISA法测定单核源巨噬细胞上清液MMP-9、TF含量。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了