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To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

After that, all samples were fixed with 10 g/L paraform for 45 minutes. Then 50 μL well-fixed blood was added into the polystyrene tube, meanwhile 20 μL monoclonal antibody, such as CD42a-b-c-d and PE-labeled CD42b, was respectively mixed gently with the blood sample and incubated at room temperature in dark for 30 minutes.

取50 μL固定后全血加至流式检测管，同时分别向不同管中加入20 μL糖蛋白Ib/IX/V 复合物单克隆抗体CD42a-b-c-d和PE标记的糖蛋白Ibα单克隆抗体CD42b，混匀后室温避光孵育30 min，然后CD42a-b-c-d标记的样品中加入20 μL FITC标记的羊抗鼠 IgG，混匀后室温避光孵育30 min。

Resibufogenin was administered into the well-grown cell cultures and incubated for 4 d.

把酯蟾毒配基加入生长状态良好的悬浮细胞中转化四天。

Phagocytes were incubated in vitro for 2 h in medium containing 10^(-3) to 10^(-6) M of zinc chloride, and 10^(-4) to 10^(-7) M of cadmium chloride. Respiratory burst activity was decreased for all concentrations studied except 10^(-7) M of cadmium chloride.

首先将头肾吞噬细胞在含有10^(-3)~10^(-6)M的氯化锌、10^(-4)~10^(-7)M氯化镉的培养基中体外培养2小时，结果除了10^(-7)M浓度氯化镉的组别外，其余组别的呼吸爆活性皆有降低的情形。

HRP was coupled with the fluorochrome FITC and encapsulated with liposome . pEGFP-N1 plasmid (expressing green fluorescent protein) with encapsulation in commercially available liposome was prepared. The eyes were enucleated 1, 4 and 8 weeks after zonule rupture and anterior segments comprising lenses were incubated in medium containing one of these components. Cryo-sections were made and translocation of fluorescent macromolecule from the medium into the lens and green fluorescent protein expression in the epithelium were observed by fluorescent microscopy.

制备FITC标记的HRP脂质体；制备pEGFP-N1质粒（表达绿色荧光蛋白的质粒），并用脂质体包裹；豚鼠悬韧带部分离断后1周、4周、8周取含晶状体的眼前段标本分别在含有FITC-HRP复合物、pEGFP-N1质粒的培养基中孵育，冰冻切片，荧光显微镜观察FITC-HRP复合物进入晶状体和pEGFP-N1质粒在晶状体内表达的情况，比较悬韧带离断侧与非离断侧的差异。3。

In spring and summer, when the water temperature is above 20 ℃, they are incubated and acceleratedly bred, with the use of HCG and LRH-A2-3 mixed or respectively.

在水温达到 20.0℃以上的春、夏季，用绒毛膜促性腺激素和释放激素LRH-A_(2-3进行催产，混合或单一使用，剂量视亲鱼性腺成熟度而定。

To study the biotransformation of deoxyaconitine in intestine, aconitine was incubated with human intestinal bacteria in vitro .

采用人肠内细菌和乌头碱体外温孵的方法，探讨去氧乌头碱在人肠内的生物转化。

To study the biotransformation of aconitine in human intestine, aconitine was incubated with human intestinal bacteria in vitro .

采用人肠内细菌和乌头碱温孵的方法及电喷雾质谱技术，探讨了乌头碱在人肠内的生物转化规律。

To study the biotransformation of 16- O -demethylaconitine in human intestine, aconitine was incubated with human intestinal bacteria in vitro .

采用乌头碱和人肠内细菌体外温孵的方法，探讨乌头碱在肠内的生物转化规律。

The third passage of cells at 2×109/L were incubated in adipogenic, osteogenic and chondrogenic mediums, separately.

收集第3代细胞，分别接种于向脂肪细胞、成骨细胞、软骨细胞分化的培养液中进行诱导。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了