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The result showed that with the increasing of density, plant height was increased, stem diameter and ear diameter was decreased, ear was shorten, rare top length、No. of ear nod、ear height was increased;brace root number and total root number was decreased, the rate of root dry weight was increased within farming layers; population dry matter accumulation was increased and plant dry matter accumulation presented the opposite trend; ear linage、kernels per ear、100-kernels weight、double-ear rate、plant yield was decreased, yield was increased and decreased successively.

结果表明:随群体密度的增加植株高度增高、茎粗、穗粗变细、果穗变短,秃尖长度、穗位节数和穗位高度呈现增加的趋势;气生根与总根数减少,耕层根系干重比例增加;群体干物质积累量随密度增加而增加,单株干物质积累量呈相反的趋势;穗行数、穗粒数、百粒重、双穗率、单株产量均随密度的增加而减低,群体产量先增后降。

Content of Chla,β-carotene and zeaxanthin of S. platensis grown in Se decreased, violaxathin, fucoxanthin, 19'-hexanoyloxyfucoxanthin and peridinin increased, Chla and Chlb of D. salina also decreased, but carotene increased, all pigments of D. zhanjiangensis increased except violaxanthin and 19'-hexanoyloxyfucoxanthin decreased. Effect of Ge on pigments of microalgaes were different in different algaes: Chla,β-carotene and zeaxanthin of S. platensis decreased, three other carotene increased, in D. salina, violaxanthin and lutein decreased, violaxathin, fucoxanthin and 19'-hexanoyloxyfucoxanthin increased, all pigments of D. zhanjiangensis increased except fucoxanthin and peridinin decreased; Chla,β-carotene and violaxanthin of Chlorella sp increased, but fucoxanthin and peridinin decreased.

暴露于硒中的钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素和玉米黄素的含量都减少,岩藻黄素、19'-乙酰氧化岩藻黄素、多甲藻素和紫黄素的含量明显增加,盐生杜氏藻的叶绿素类也减少,但类胡萝卜素的含量则增加,湛江叉鞭金藻(D.zhanjiangensis)中除紫黄素和19'-乙酰氧化岩藻黄素有减少外,其它色素都增加;锗对四种微藻光合色素的影响也不尽相同,钝顶螺旋藻(S.platensis)的叶绿素A、β-胡萝卜素及玉米黄素含量减少,岩藻黄素、19'-乙酰氧化岩藻黄素和紫黄素的含量增加,盐生杜氏藻中除紫黄素和叶黄素有增加外,其它色素都减少,湛江又鞭金藻(D.zhanjiangensis)正相反,除岩藻黄素和多甲藻素有减少外,其它色素都增加,小球藻的叶绿素A、β-胡萝卜素和紫黄素增加较大,但岩藻黄素和多甲藻素则减少。

24H treatment group compared with 24h model group: the density of light of HSP70 of the group increased and which of TGF-β〓, is not different with the model group; the density of the light the HSP70 of the Baicalin group increased and which of it the TGF-β〓, increased with the model group; the density of light of the Concha Margatitifera Usta is not different and which of TGF-β〓, increased with the model group; the density of light of the Cholic Acid group is not different and the density of light of it of TGF-β〓 increased with the model group; the density of light of the Hefang Group is not different and which of TGF-β〓 is not different than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组无显著性差异(p>0.05)。本实验结果发现,与单纯缺血12小时、24小时大鼠相比,药物干预组缺血侧大脑皮层TGF-β1免疫反应明显增强,计算机辅助图像半定量分析两组间缺血侧大脑皮层TGF-β1免疫阳性细胞数差异非常显著。说明药物对局灶性脑缺血损伤可能有一定保护作用,其机制可能与诱导脑细胞TGF-β1合成增加有关。然而,药物调控TGF-β1表达,其促TGF-β1表达增强的机制可能是这样的:脑缺血时TGF-β1mRNA表达增加可能与缺氧诱导神经和胶质细胞刺激生长因子表达有关。

12H treatment group compared with 12h model group: the density of light of HSP70 of the Gardenoside group is not different and which of it of TGFβ〓 increased with the model group; the density of light of the HSP70 of the Baicalin group in not different and which of the TGF-β〓 is no different with the model group, the density of HSP70 of the Concha Margatitifera Usta is not different and which of TGFβ〓 increased than the model group; the density of the HSP70 of the Cholic Acid group is no different and the density of TGF-β〓 increased than the model; the density of the light of HSP70 of the Hefang Group increased and which of TGF-β〓 increased than the model group.

合方治疗脑缺血12小时组缺血脑组织TGF-β1光密度值较缺血12小时模型组明显升高(p<0.01)。大鼠局灶性脑缺血24小时模型组缺血脑组织TGF-β1光密度值含量较正常组明显增高(p<0.01)。珍珠母治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。胆酸治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。栀子苷治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组无显著性差异(p>0.05)。黄芩苷治疗脑缺血24小时组缺血脑组织TGF-β1光密度值较缺血24小时模型组明显升高(p<0.01)。

For whatever reasons, the number of disabled beggars bumming along the street is rapidly increased.

不论出于什么原因,其数量沿街流浪迅速increased。

The experiment two: enzyme preparation significantly improved average daily gainand feed conversion ratio (P<0.05). Enzyme preparation significantly increased energymetabolizability and digestibility of crude fiber, crude protein and neutral detergent fiber,but had no remarkable effect on digestibility of dry matter, crude fat and acid detergentfiber. Enzyme preparation significantly decreased the relative viscosity of duodenal andjejunal digesta. The pH of intestine had no noticed difference in all groups. Enzymepreparation significantly decreased relative weight of gizzard, proventficulus, duodenum,jejunum and ileum. Enzyme preparation significantly increased villus size of duodenumand jejunum, and villus to crypt ratio of duodenum and ileum significantly increased too.Enzyme preparation considerably decreased ileal crypt height (P<0.05), and didn"t affectthickness of intestinal wall. Supplementing enzyme preparation, the serum glucose, totalprotein and alanine aminotransferase, but enzyme preparation hadn"t noticed influenceupon uric acid, total cholesterol, triglyceride and high-density lipoproteins. Enzymepreparation significantly increased insulin, triiodothyronine and insulin-like growthfactor-Ⅰ. Adding enzyme preparation, the percentage of thyroid stimulating hormone andgrowth hormone in the serum increased 16.44%, 19.18% and 18.84%, 21.74%respectively, and the percentage of glucagon and thyroxine decreased 12.07%, 14.36% and 13.79%, 15.40%, but failed to reach statistical significance (P>0.05). Enzymepreparation significantly increased (P<0.05) the trypsin and amylase activity of duodenaland jejunal digesta, but enzyme preparation didnt affect significantly (P>0.05) theintestinal lipase activity and pancreatic digestive enzyme. Enzyme preparation had nosignificant effect on caecal microbial population.

试验二:酶制剂显著提高平均日增重和饲料转化率(P<0.05);酶制剂显著提高能量代谢率及粗纤维、粗蛋白、中性洗涤纤维消化率(P<0.05),而对干物质、粗脂肪、酸性洗涤纤维消化率影响不显著;酶制剂显著降低十二指肠和空肠食糜相对粘度(P<0.05);添加酶制剂对肠道pH影响不显著;酶制剂显著降低肌胃、腺胃、十二指肠、空肠、回肠相对重(P<0.05),显著提高十二指肠和空肠绒毛高度,显著增加十二指肠和回肠绒毛高度/隐窝深度,降低回肠隐窝深度(P<0.05),对肠壁厚度影响不显著;酶制剂显著提高血清葡萄糖、总蛋白和谷丙转氨酶浓度(P<0.05),对尿酸、总胆固醇、甘油三酯及高密度脂蛋白浓度影响不显著,显著提高胰岛素、T_3、IGF-Ⅰ水平,添加酶制剂后,促甲状腺激素、生长激素分别提高16.44%、19.18%和18.84%、21.74%,胰高血糖素和T_4分别降低12.07%、14.36%和13.79%、15.40%,但差异不显著;酶制剂对胰腺消化酶活性影响不显著,显著增加十二指肠和空肠胰蛋白酶、淀粉酶活性,对小肠脂肪酶活性影响不显著;酶制剂对盲肠微生物菌落数影响不显著。

It is shown from the assessment results that (1) both the TN and TP fluxes discharged from the Changjiang River to the Changjiang River estuary sea area increased continuedly, the DIN concentration in the sea areas increased continuedly, and the Si concentration decreased continuedly, which resulted in the significant changes in N/P/Si ratios;(2) the chlorophyll a concentration in the sea areas increased continuedly, and the significant changes in community structure of phytoplankton occurred;(3) the species and biomass of benthos declined greatly, and the bottom oxygen-poor water area increased significantly;(4) the range and occurrence frequency of red tide events in the sea areas increased greatly, and the algal toxic infection events of mussel were frequently found in the sea areas.

结果表明,长江向长江口海域输送总氮和总磷通量持续增大,长江口及其邻近海域无机氮浓度持续增高而硅浓度持续下降,并由此导致N/P/Si与比值的显著变化;该海域叶绿素a浓度持续增大,浮游植物群落结构也发生了显著变化;该海域底栖生物种类和生物量都大大减少,底层水低氧区面积也显著扩大;该海域赤潮事件无论是规模还是频率都大大增加,藻类毒素DSP/PSP贻贝传染事件也时有发生。

The Go moved backward with 1.35mm and upward with 1.90mm in group I, the gonial angle increased for 3.69°, and angle between palatal plane and mandibular plane was increased for 5.00°; In group II, the Go moved backward with 1.85mm and upward with 3.32mm , the gonial angle increased for 4.06°, and angle between palatal plane and mandibular plane was increased for 5.22°. Both in group I and II, there was a tread for clockwise rotation of mandibular corpus. The length of pterygomasseteric sling was mild increased due to the backward movement of Go.

在Go点的变动方面,在Me点向前位移的族群里,术后Go点平均向后1.35mm,向上1.90mm, gonial angle 平均增大3.69度,腭平面与下颚平面的夹角则是增加5.00度;在Me点向后位移的族群里,术后Go点平均向后1.85mm,向上3.32mm, gonial angle 平均增大4.06度,腭平面与下颚平面的夹角则是增加5.22度,不论是向前或是向后的族群,结果皆显示翼咀嚼肌悬吊带有轻微被拉长的迹象。

The increased amount of non--exchangeable NH4^+-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^+-N (P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively). NH4^+-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^+-N increased significantly when added (NH4)2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^+-N from (NH4)2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^+-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d (P=0.0434), but not significantly at 40 d and 60d (P=0.7378 and 0.5375). The increased amount of non-exchangeable NH4^+-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^+-N, the increased amount of non-exchangeable NH4^+-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^+-N的增加量均表现为以土垫旱耕人为土最大,其次是黄土正常新成土,简育干润均腐土和干湿砂质新成土较小;添加有机物料极显著影响培养期间的非交换性NH4^+-N增加量(培养20d、40d和60d时P分别为0.0002,0.004和0.0003),表现为紫花苜蓿和长芒草土壤非交换性NH4^+-N的增加量均极显著高于不添加有机物料的对照土壤;在培养20d、40d和60d时,加(NH4)2SO4土样非交换性NH4^+-N的增加量显著大于不加(NH4)2SO4土样(户分别为0.0037,0.0033和0.0027),这是土壤对(NH4)2SO4中NH4^+-N固定的必然结果;不同植被类型土壤培养20d时的非交换性NH4^+-N增加量差异显著(P=0.0434),培养40d和60d时差异不显著(p分别为0.7378和0.5375);长期秸秆和氮肥配施土壤非交换性NH4^+-N增加量大于不施肥对照土壤,但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^+-N含量均呈极显著正相关;而非交换性NH4^+-N的增加量与粘粒无相关性,但与全氛和有机质呈显著正相关。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。

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