查询词典 inclusion body

There were amaranth inclusion body in rotundity and ellipse with the size of 2~5 μm in pyramidale cytoplasm in hippocampus of cerebral cortex in shepherd dog with rabies.

在患狂犬病的牧羊犬大脑皮层海马的锥体细胞浆内有2~5μm的圆形或椭圆形紫红色的包涵体。

Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I，并成功地在原核细胞E.coli进行了重组表达，筛选出稳定表达的工程菌株DH5α，摸索了稳定表达的条件，摸索了包涵体的洗涤、变性和复性条件，摸索出了复性蛋白Src I的纯化工艺，纯化过程简单，经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化，就可获得了高纯度的重组蛋白样品(纯度达99％以上)，该纯化方法适合重组蛋白的大量纯化，为Src I的性质、结构和功能研究奠定了良好的基础。

Among these systems, the expression level of ZNF191(243—368) was increased in inclusion body system under a higher isopropylthio-β- D -galactoside concentration, but the non-target proteins were also increased more, which made its purification more difficult and the yield lower.

为了能够得到充足的锌指蛋白ZNF191(243-368)来研究其性质、结构和功能间的关系，本文研究了包涵体、谷胱甘肽 S－转移酶和组氨酸标签三个表达体系来表达ZNF191(243-368)。

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

Methods Split the recombinant E. coli for expression of rhOP-1 by ultrasomication in ice bath and extract the inclusion body. Dissolve the inclusion body with 8mol/L urea, purify by SP-Sepharose chromatography, refold by gradient dialysis.

将表达rhOP-1的大肠杆菌菌体在冰浴下超声裂解，分离提取包涵体，用8mol/L尿素溶解，纯化后进行梯度透析复性。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

The scFv formed inclusion body in E. Coli. 8 mol/L urea was used to solubilize the inclusion body, and the scFv was then purified in two steps , including Ni 2+ chelating affinity chromatography and gel filtration.

大肠杆菌表达的单链抗体包涵体，用8 mol/L尿素裂解，经过Ni离子螯合亲和层析和凝胶过滤两步纯化后，纯度达到97%以上，再通过透析复性除去尿素。

Another part was expressed in the precipitation as the way of inclusion body. The inclusion body was purified by urea solution of different concentration and denaturalized, naturalized.

经初步鉴定后，证实表达蛋白部分位于裂菌处理后的上清中，部分以包涵体的形式存在于沉淀中。

Hypernym inclusion body , cellular inclusion , inclusion

曾用名&杜顿巨细胞&。。。。拉塞尔小体 russell's body 。。。曾用名&卢梭小体&。

It was expressed as inclusion body and was purified by Ni-NTA Column Affinity Chromatography under denaturing conditions. The inclusion body was then refolded by two different ways, both of which can produce activated enzyme.

在变性条件下，包涵体经镍次氮基三乙酸柱层析纯化，并以两种不同的方法进行复性，均可获得具有活性的HaSNPV几丁质酶。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。