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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Combat fader will fade out all your frames while they are inactive and fade them back in once you are in combat or active.

" = "战斗渐隐将渐隐你的所有的头像，并当你进入战斗或单位激活后恢复透明度。

But if you're physically inactive and fatigued, being just a bit more active will help.

" "但如果您身体不活跃和疲倦，只是有点更积极的将帮助。

Use a plastic worm with a glass bass fishing techniques bead between the worm and the weight for inactive fish.

用塑料蠕虫一杯低音捕鱼技术珠串之间蠕虫和鱼体重活跃。

Use a plastic worm with a glass bead between the worm bass fishing poem and the weight for inactive fish.

用塑料蠕虫与蠕虫之间玻珠低音捕捞鱼诗和体重活跃。

In our study, HaPLE2 was observed in all individuals within the three populations,but HaPLE1 was not. Generally,inactive copies of a transposable element will be fixed or lost in the genome of individuals in a population over time if they are neutral.

因为当一个转座子失去活性时就被固定在基因组上，这个基因也会由于遗传而出现在下一代的基因组中，当在室內连续饲养几代之后，这个基因就会存在于几乎所有的个体中，而HaPLE1只存在于24-35%的棉铃虫个体的基因组中。5 piggyBac转座子基因的亲缘关系树分析收集各种数据库登录的来自11种生物的19个piggyBac类似因子的氨基酸序列，利用CLUSTALX1.8和MEGA3.1构建亲缘关系树，进行系统进化分析。

A software and a relevant method are designed and set up for the calculation of the relative content of the inactive PS Ⅱ center, by using which this study can be accomplished A new non-modulation fluorometer with its data acquisition and date analysis software is also designed and assembled

此外，也参加了新的非调制式动力学荧光计的研制及其软件的编写。

A data acquisition and data analysis software for the fast fluorscence kinetics fluorometer of our laboratory is redesigned and recompiled using assembly and BASIC language. The data acquisition rate in the fast rising part of the fluorescence kinetics curves is one order faster (100μs/point) than the old one. It can measure fluorescence parameters, such as Fo and Fi more accurately for calculation of the relative content of inactive PSⅡ, etc.

用N80-BASIC语言结合汇编语言重新编写了本室快速叶绿素动力学荧光计的测控程序，使快速荧光上升曲线的采样速度提高了一个数量级（达到100μs／点），可对Fo、Fi等关键荧光参数进行精确测定，为无活性PS Ⅱ中心相对含量的准确测定奠定了基础。

Mrs Reed's hand still lay on her work inactive; her eyes of ice continued to dwell freezingly

里德太太的手还一动不动地搁在她的活计上；她那冰冷的眼睛还冷冷地盯着我。

Mrs. Reed's hands still lay on her work inactive: her eye of ice continued to dwell freezingly on mine.

里德太太的手仍一动不动地放在她的活儿上，冷冰冰的目光，继续阴丝丝地凝视着我。

We got alerted a couple of times while we were down south that HETs were on the way to bring us back up north because things were going to go hot again, but it was just rumors.

南下的途中我们不只一次得到警告说重型装备运输车将拉着我们重新北上，因为局势正在变得重新紧张起来，但这只是谣传。

It's the one where they find the ghost in the salt mine.

这一集是演他们在盐矿找到鬼