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To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性,我们利用原位杂交和免疫组化等方法,研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动,结果表明:(1)热对各阶段的雄性生殖细胞都有损害,粗线期的初级精母细胞尤为敏感,实验性隐睾内的精原细胞尚未完全受到"致命"影响,说明精原细胞对热有一定的耐受性,但即使成为粗线期/双线期初级精母细胞,却未能获得由有丝分裂过渡到减数分裂的能力,呈现不同程度的凋亡,所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞,更谈不上长形精子细胞和精子的形成;(2)腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录,说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的;(3)正常兔睾丸组织中,〓在精原细胞和粗线期/双线期精母细胞中均有表达:cyclin B1蛋白在减数分裂前期的粗线期/双线期初级精母细胞中的表达量增加,于变态末期的精子细胞中达高峰。

The effect of tribology factors on bearing vibration is studied experimentally. AR model and WPT are used to processing the normal arid abnormal signals. The results are as follows:1. The vibration of ball bearings is caused by the tribology action of contact pairs of ball and ring races and has the essential of tribo-dynamics. Any factors affecting the tribology characteristics of contact pairs will affect vibration and noise of bearings consequently.2. Vibration characteristics of bearing keep almostly unchanged with the increasing of axial load expecting the increasing of nature frequencies. When radial load increasing with axial load unchanged, nature frequencies keep unchanged but some new vibration peaks appears in the spectrum.3. Vibration amplitudes are damped and nature frequency are enhanced with sufficient lubrication. Vibration and noise of bearings increase obviously and roar can happen and the contact surfaces scratch slightly under insufficient and unclean lubrication state. Bearing will be disabled in a few minutes without any lubrication.4. Bearing vibration is unstable under low speed. With the speed increasing, the vibration become stable and natural frequencies increase slightly but the amplitudes increase apparently.5. Bearing vibration can be excited only by some harmonics ofwaviness without lubrication and by all harmonics with lubrication based on the theoretical analysis.6. The nature frequencies of ball bearings decrease with the increasing sizes. When international clearance increasing, nature frequencies decrease in radial and increase in axial and angular and the amplitudes increase in radial and decrease in axial and angular. When the number of balls increasing, nature frequencies increase and amplitudes decrease. When the pitch diameter increasing, axial nature frequency increase and others nearly keep no change. When outer race groove curvature radius increasing, nature frequencies increase in radial and decrease in axial and angular and amplitudes keep no change in radial and increase in axial and angular. When inner race groove curvature radius increasing, nature frequencies decrease and amplitudes increase.7. The distortion in amplitude and frequency components of bearing vibration signals picked up by the present probe measurement method is founded and right conclusions cannot be achieved by the signals. When accelerometer is rigidly screwed with steel stud onto a flat outer surface of a ball bearing, vibration signal can be got without distortion and the reliability of research on bearing vibration is assured.8. AR model is suitable for large samples of bearing vibration signals, square root of length of samples can be used as the upper limitation of order determination and the FPE order selection criterion is effective. Many advantages of AR spectrum are founded over the classical based on FFT.9. Time-Frequency analysis is necessary for abnormal noise of ball bearings. WPT overcomes the principle shortcomings of STFT and proved to be a best tool to process the abnormal signals.

理论分析和试验研究表明: 1球轴承振动是钢球—滚道接触副中的各种摩擦学作用引起的,具有摩擦动力学的本质,任何对接触副的摩擦学特性有影响的因素都将对球轴承的振动和噪声特性产生影响; 2中心轴向载荷作用下,载荷增大使球轴承的固有振动频率升高,载荷越大同样的载荷增幅引起的频率升幅减小,足够大的中心轴向载荷作用下载荷的变化对球轴承振动的频率特性不会产生明显的影响; 3轴向和径向联合载荷作用下,径向载荷不大时球轴承振动的固有频率基本不变,但是可能引起变刚度激励的非线性振动,出现新的频峰,过大的径向载荷将使部分钢球脱离接触,使球轴承的振动和噪声呈现不稳定状态; 4润滑对球轴承的振动和噪声特性有重要的影响,良好的润滑对振动有明显的抑制作用,润滑不充分时,振动和噪声的水平会有明显增高,一定条件下还会激发接触副中的谐振,发出啸声,造成接触表面的伤害,无润滑干接触时,短时的运转就会损伤接触副表面,使振动和噪声迅速增大,并随时可能引发严重的磨损和卡滞失效;接触副润滑良好时,油膜的"刚化效应"使球轴承振动的固有频率有所提高,润滑剂中含有弥散性污浊时,振动的幅度总体升高,但弥散性污浊不会改变球轴承振动的频率特性;浙江大学博士学位论文:球轴承振动的研究 5低速时,球轴承振动的基本特征呈现不稳定状态,随着转速的提高,球轴承振动的频率特性趋于稳定,固有频率频峰升高; 6理论分析表明,干接触时钢球和滚道表面波纹度的某些谐波分量能激励球轴承的振动,振动的幅值与谐波幅值成正比,实际球轴承中钢球的分布不可能完全均匀,波纹度的激励作用会随时发生;润滑接触的分析表明,波纹度的任意谐波分量均能激励球轴承的振动; 7球轴承的几何和结构参数分析表明,球轴承尺寸越大,径向、轴向和角振动的固有频率越低;钢球中心圆直径增大,球轴承的径向和轴向振动的固有频率基本不变,角振动的固有频率有所上升,振动的幅频特性基本不变;径向游隙加大使球轴承的径向振动固有频率降低,轴向和角振动的固有频率有所升高,径向振动幅频特性的幅值升高,而轴向和角向降低;钢球的数量增多使球轴承振动的固有频率上升,幅频特性的幅值下降,径向振动的幅值下降最为明显;外圈沟曲率半径系数增大使球轴承径向振动的固有频率升高,轴向和角振动的固有频率降低,径向幅频特性基本不变,轴向和角向幅频特性幅值升高;内圈沟曲率半径系数增大使径向、轴向和角振动固有频率均下降,振动幅频特性的幅值均有升高; 8试验对比表明,传感器采用探针式安装时,由于探针接触副接触特性的影响,钡(量得到的球轴承振动信号有失真,采用专门设计和制作的试验球轴承,以固定式安装加速度计,首次测量得到了球轴承振动的真实信号,通过对振动信号的分析,验证了球轴承振动的理论模型; 9)基于时间序列分析的AR模型适用于大样本球轴承振动信号的分析,以样本长度的均方根值作定阶上界,FPE做判阶准则,给出的AR谱光滑,频率分辨率高,是球轴承振动分析的简便而可靠的手段; 10以时频域分析的小波包变换分析球轴承异音信号能够比较好地定位和聚焦异音发生的时间,时间间隔,频率范围,同样是球轴承振动分析的可靠的手段,可用作球轴承故障诊断技术。

In accordance with the Provisions on the Protection of Geographical Indication Products issued by National Bureau of Quality Inspection, by the end of 2009, 21 kinds of products in the province had obtained the protection of national geographical indication products including Zanhuang Chinese Date in Shijiazhuang Area; Shacheng Grape Wine, and Xuanghua Milk Grape in Zhangjiakou Area; Shanzhuang Old Wine, Weichang Potato, Chengde Guoguang Apple in Chengde Area; Changli Grape Wine, Lulong Silk Noddles, and Shimen Walnut in Qinhuangdao Area; Lixian County Chinese Yam, and Rongcheng Bamboo Shoot in Baoding Area; Botou Pear, Cangzhou Golden-Silk Jujube, and Huanghua Winter Dater in Cangzhou Area; Ningjin Pear in Xingtai Area; Shexian County Walnut, Shexian County Pepper, Weixian County Pear, Yongnian Garlic, and Wu'an Millet in Handan Area; and Jingdong Chestnut (including the administration regions of Qianxi, Qian'an and Zunhua in Tangshan Area, Funing, Qinglong, and Lulong in Qinhuangdao Area, Xinglong, Kuangcheng, Pingquan, Chengde, Luanping, and Fengning in Chengde Area).

按照国家质检总局颁布的《地理标志产品保护规定》,截至2009年底,我省石家庄地区赞皇大枣;张家口地区沙城葡萄酒、宣化牛奶葡萄;承德地区山庄老酒、围场马铃薯、承德国光苹果;秦皇岛地区昌黎葡萄酒、卢龙粉丝、石门核桃;保定地区蠡县麻山药、容城绿竹笋;沧州地区泊头鸭梨、沧州金丝小枣、黄骅冬枣;邢台地区宁晋鸭梨;邯郸地区涉县核桃、涉县花椒、魏县鸭梨、永年大蒜、武安小米;京东板栗(唐山地区的迁西、迁安、遵化,秦皇岛地区的抚宁、青龙、卢龙,承德地区的兴隆、宽城、平泉、承德、栾平、丰宁等行政区域)共计21种产品已获得国家地理标志产品保护。

The industry magnesia mainly uses in making the ceramics, the enamel, the fire-proof material and so on; In the polishing compound, the adhesive, in the paint manufacture takes the stopping; In the artificial fiber, the rubber (chloroprene rubber, fluorine rubber) make the promoter and the catalyst; Makes the antacid on the medicine in the laxative, uses in treating the hyperacidity, the stomach and duodenum ulcer; May take the hard sugar purification is the decolorant in the food processing, the ice cream pH value conditioner and so on; In the construction profession, the magnesia may the manufacturing contain the magnesium special cement and the heat preservation board and so on; The biggest use is serves as the fertilizer and domestic animal's feed in the agriculture, causes the plant and in the animal metabolism process principal element, when serves as cow's feed, may prevent because to lack nervous system function being out of balance which the magnesium causes, namely the so-called forage grass convulsion gets sick; Uses in the chemical industry making the metal magnesium and the magnesium chemical, like magnesium sulfate, magnesium chloride as well as other chemicals; Moreover also uses in the water treatment, the detergent which the flue was mad uses in the glass, the dye, the electric cable, the silcon steel industry, the uranium processing, the electronics industry, the insulation material industry, as well as uses in professions and so on petroleum chemical additive, casting, phenolic plastic.

工业氧化镁主要用于制造陶瓷、搪瓷、耐火材料等;在抛光剂、黏合剂、油漆的制造中作为填充料;在人造纤维、橡胶中作促进剂和催化剂;在医药上作抗酸剂于轻泻剂,用于治疗胃酸过多、胃和十二指肠溃疡病;在食品加工上可作为砂糖精制是的脱色剂,冰淇淋的pH值调节剂等;在建筑行业中,氧化镁可生产制造含镁特种水泥和保温板等;在农业上最大用途是用作肥料和牲畜的饲料,使植物和动物代谢过程中的主要元素,用作奶牛的饲料时,可防止因缺镁而引起的神经系统机能失调,即所谓牧草性痉挛病;在化学工业中用于制造金属镁和镁化学品,如硫酸镁、氯化镁以及其他化学品;另外也用于水处理,烟道气的洗涤剂用于玻璃、染料、电缆、硅钢工业、铀加工、电子工业、绝缘材料工业,以及用于石油添加剂、铸造、酚醛塑料等行业。

The selection of geological prospecting in different stages(initial-prospecting, detailed-prospecting)(2)the selection of geological prospecting in the deep-laid and shallow-laid of tunnel project (3)the selection of geological prospecting of tunnel projecting in different geological conditions Finally, the author summed up main problems needing to be solved in tunnel engineering geological prospecting as follow:(1)differentiate stratum and find out the depth and thickness of different stratums in the tunnel section (2)find out geological structure and existence situation of fault, broken zone and weak plane (3)find out weathering level of bed rock to classify surrounding rock combining speed materials (4)differentiate stratum structure of the entrance of tunnel to judge the stability of it (5)find out existence situation and performance of ground water (6)situations of other harmful geological phenomenon In view of above problems and classification of surrounding rock, the author showed principles of rational choosing of methods in geological prospecting and counted disperse range of physical character parameter value in different petrosal character, getting achievements as follow:(1)summarization of effect, merits and demerits of different geological prospecting methods in the tunnel geological investigation (2)method selection and arrangement principle of tunnel engineering geological prospecting in different geological conditions and prospecting stages (3)According to previous experience and cases, the author concluded the物性parameters most in use ,and showed the basis of selecting geological prospecting methods in different geological conditions.

论文中用几个应用实例来说明:(1)不同勘察阶段物探工作的选用;(2)深埋、浅埋隧道工程物探工作的选用;(3)不同地质条件隧道工程物探工作的选用。最后总结归纳出隧道工程物探所需要解决的问题主要有:(1)划分地层,查明隧道通过剖面的各地层深度与厚度。(2)查明地质构造,隧道轴线段有无断层、破碎带、软弱层面等。(3)查明基岩风化程度,从而结合速度资料进行围岩分级。(4)划分洞口地层结构,判断洞口稳定性。(5)查明地下水赋存情况及其活动动态。(6)其他不良地质现象存在情况。针对上述需要查明的地质问题与计算围岩分级情况,论文提出合理选用物探方法的原则,并统计了物性参数值在不同岩性中的分布范围。得出以下研究成果:(1)总结出各物探方法在隧道地质调查中的作用和优缺点。(2)得出不同地质条件下和不同勘察阶段,隧道工程物探方法选择和布置的原则。(3)根据以往工作经验和事例,对常用的物性参数进行了归纳总结,同时介绍了不同地质条件下物探方法选择的依据。

Results Punctiform erosion, hemorrhage were observed by nake eyes in each groups. Epithelium cellular necrosis, defluxion, neutrophil being dominant in propria lamina and intact glandular were observed with microscopy. The erosion became severe with the successive stimulate of ethanol. There is no difference between distillate spirit and sodium chloride rats in Guth in 7th day, but Guth in distillate spirit rats was higher than that in sodium chloride rats in 30th day. PGE2, TGF-α contents in gastric mucosa of treated group increased significangly compared with control group. PGE2 showed no difference between each group in 7th and 30th day; TGF-α in gastric mucosa of distillate spirit and sodium chloride rats decreased after 1 month.

结果 茅台组、乙醇组、生理盐水组胃黏膜均肉眼可见程度不同的点状糜烂、出血;光镜下黏膜部分上皮坏死脱落,固有层以中性粒细胞为主炎性细胞浸润,腺体结构基本完整,病变随时间延长有所加重,Guth积分1周茅台组与生理盐水组差异无统计学意义,1月两者相比差异有统计学意义;茅台组、乙醇组胃黏膜组织内PGE2水平与生理盐水组相比明显增高,同时TGF-α表达增强;但PGE2水平各组间1周与1月相比差异无统计学意义;TGF-α表达强度在茅台组、生理盐水组1月后逐渐降低,差异有统计学意义。

Results the basic CT features of 102 patients with Peripheral lung adenocarcinomas were as follows:vacuole or air bronchogra charging sign was found in which as were seen in 24 cases (80%) in high differential adenocarcinoma and were seen in 10 cases (30.3%) in moderate differential adenocarcinoma but not seen in low differential ones, spiculation sign in 73(71.5%). spiculate protulerance sign in 52 (50.9%). lobulation sign in 79(77.4%), pleural in dentation sign in 65(63.7%), vessel convergenoe sign in 69 cases(67.6%), and calcification inside the lesion was 31 cases. 67 cases had more then three basic CT signs mentioned above.

结果 102例周围型肺腺癌的主要CT分析征象是:空气支气管征或空泡征、高分化腺癌24例(80%)、中分化腺癌10例(30.3%)、低分化腺癌无此征、毛刺征73例、棘突征52例、分叶征79例、胸膜凹陷征65例、血管集束征69例、病灶内钙化31例,其中67例出现上述三种以上征象。

Seizure frequency decreased by at least 50% in 47% of patients, by at least 75% in 24% of patients, and by 100% in 7% of patients. Of those receiving oxcarbazepine monotherapy, 59% had at least a 50% reduction in seizure frequency, 36% had at least a 75% reduction in seizure frequency, and 10% were seizure-free. Adverse events reported most frequently were dizziness in 46%, headache in 32%, fatigue in 30%, diplopia in 30%, nausea in 26%, abnormal vision in 21%, and somnolence in 21%.

结果在全部完成试验的患者中,47%的患者其发作频率下降至少50%,24%的患者发作频率下降至少75%,7%的患者2年间不再发作;而仅服用oxcarbazepine的患者中,59%的患者其发作频率下降至少50%,36%的患者其发作频率下降至少75%,10%的患者其发作频率100%下降;在副作用的部分,常见的有晕眩46%、头痛32%、疲倦30%、复视30、恶心26%、异常的视觉21%,以及嗜睡21%。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病,1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖;应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia;一色恤~﹁染澎糕行HE染色观察组织病理学孪叱;胶质细胞原纤维酸性蛋白protellZ,Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分,应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

In such doing, this dissertation serves as a step stone for papers of its counterparts to come, and, more importantly, it proposes a strategic alternative to the realization of models for image processing. This dissertation consists of three major parts. In the first part, detailed discussions and delicate analyses of academic papers on Cellular Neural Network will be provided in the hope of helping us see the potentiality of Cellular Neural Network in the applications of image processing. I will focus on the aforementioned limitations on hardware compilation as well. In the second part, I will put forth "texture analysis" as one basic model of analysis when we apply Cellular Neural Network to image processing. In this so-called texture analysis, a useful "spatial feature" is especially drawn to help us overcome possible problems of more complicated Cellular Neural Network applications in image processing."Spatial feature" also serves as a well-functioning mechanism for technology of image identification. In the last part of this thesis, I will look into a case study, where Cellular Neural Network is applied to help de-screen document image. Using it as an example, we will see how algorithms of Cellular Neural Network may be of marvelous use in applications in document image processing, since it would reduce a great deal of calculation and computation when applied to software compilation, yet opens up unlimited possibilities for higher-speed hardware compilation of high-level image processing.

这篇论文主要可以分为三大部分:在第一部份里,我们会详细地说明并讨论在过去到现在大部分将分子类神经网路应用於影像处理的相关文献及未来所有可能的发展和技术,另外也将分子类神经网路作一完整的介绍,除此之外,我们也会特别著重於分子类神经网路在影像处理相关应用理论的讨论以及其硬体实现化的考量;在第二部分里,我们提出了一个将分子类神经网路应用於影像辨识处理的基础分析—纹路分析,这是由於纹路分析的复杂性和普遍性会使得分子类神经网路於高阶影像处理的应用不会只局限在单一的影像处理技术,其中我们也提出了一个相当有用的空间特徵,此一特徵不但可以使复杂地高阶影像处理能够应用分子类神经网路,也为影像辨识技术提供了一个很好的辨识机制;在最后一部分里,我们也将文件影像分析做了一个完整的剖析,并以文件影像的去网点为例来说明在实际情况下的分子类神经网路的应用,如此演算法的开发也为文件影像处理提供了更多实际的应用,更考量了文件影像处理若以软体实现时的计算量负荷,而对未来高阶数位影像处理能够以硬体实现来提高处理速度提供了无限的可能。

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