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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

The results were as follow: At fine, cloudlet and cloud weather in rainy season,the mean value of CO 2 content varied in a range of 349 to 350ml/m\+3 at 2.8m above the canopy, 346 to 348ml/m\+3 at 0.8m above the canopy, 345 to 349ml/m\+3 at 16m inner the forest and 352 to 357ml/m\+3 at 5m above ground in the period from 9:00 to 18:00.The CO 2 content in rainy season was lower than that in dry season. At the same weather and space-time, CO 2 content in rainy season was higher than that of CO 2 in dry season at both day and night. According to the grads calculation, there were the CO 2 flux from atmosphere to canopy with 0.61×10 -6 and 0.71×10 -6kg/(m 2·s) during day time in dry and rainy seasons respectively.

采用梯度法及CI-301PS CO2系统，实现了海南尖峰岭热带山地雨林近冠层CO 2、微气象因子梯度观测研究，结果表明：雨季晴、晴间少云及多云天气，9：00～18：00CO 2浓度平均值分别在349～350ml/m3(冠上2.8m)、346～348ml/m3(冠上0.8m)、345～349ml/m3(林内16m)、352～357ml/m3(林内5m)，较旱季相应天气分别小14～17.5、10.1～23.7、1 6.4～35.7和18.1～36.1ml/m3；1：00～8：00，则雨季CO2浓度大于旱季；梯度浓度廓变量和实时动力计算反映出，8：00～18：00CO2通量由大气向林冠层，旱、雨季平均CO2通量分别为0.61×10-6kg/(m2·s和0.71×10-6kg/(m 2·s)，19：00通量甚微，20：00～7：00CO2通量则由林冠向大气，平均CO2通量分别为0.36×10-6kg/(m2·s)和0.32×10-6kg/(m2·s)；雨季昼夜大气流向冠层的净CO2通量是相应旱季的1.56倍。

To mention a few instances of this -- Saints Columba in Scotland, Augustine in England, Boniface in Germany, Ansgar in Scandinavia, Swithbert and Willibrord in the Netherlands, Rupert and Emmeran in what is now Austria, Adalbert in Bohemia, Gall and Columban in Switzerland, were monks who, by the example of a Christian society, which they and their companions displayed, led the nations among whom they lived from paganism to Christianity and civilization.

举几个例子本-圣科伦巴在苏格兰，英格兰，奥古斯丁，博尼法斯在德国， A nsgar在斯堪的纳维亚半岛， S withbert和威利布罗德在荷兰，鲁珀特和E mmeran在现在的奥地利，阿德尔伯特在波西米亚，戈尔在和C olumban瑞士，谁是僧侣，例如由基督教社会，他们和他们的同伴显示，导致国家间他们住来自异教的基督教和文明。

Jade Wang said that this year's "11" Hainan has three Golden Week better than in previous years, the first tourism infrastructure this year than in previous years has made substantial progress, star hotel has strengthened internal management, the number of hotel increase in good reception conditions than in the past; Second Haikou to combat the "black car","Black Guide" to create a good tourism environment, which will enable tourists to enjoy the real scenery in Hainan has been genuine leisure, so this year's Golden Week is expected to complaint rate of travel will also be lower than in previous years; Third Island Tourism Festival in November and soon going to host beauty pageant are golden week will play a role in promoting tourism.

翡翠王学贤说，今年的" 11 "海南有三个黄金周较往年一样，第一次旅游基础设施，今年比前几年有了长足的进步，星级酒店已加强内部管理，一些酒店增加了良好的接待条件比过去；第二海口打击"黑车"，"黑导游"，以创造一个良好的旅游环境，使游客享受到真正的风景在海南已真正的休闲，因此今年的黄金周，预计旅游投诉率也将低于往年；第三岛旅游节即将在11月要举办选美大赛的黄金周将发挥作用，促进旅游。

Concept; In 1989 GRiD Corporation promoted first section to havetouches the type screen then to take along the computer; In 1992,Microsoft has promoted "Windows for Pen", after but two years sufferthe elimination; In 1996 Palm developed the first section successapplication to touch the pen type computer; In 1997 Oracle joinedMicrosoft the ranks to start to study the dull technology; 2000 is oneof plate computer important turning points, Bill Gates starts by 2000,continuously at in two session of Comdex congress speech vigorouslyinitiates the plate computer; In 2002, in plate computer history amost important year, also is a year which most bustles about,This year in May, Gates brings 150 plates computers to attend theMicrosoft year CEO summit, meets in August, plate computer software isbeing open to the computer manufacturer, follows closely in November,the plate computer officially is going on the market, seven bigcomputers manufacturers develop this new product, in December theplate computer Chinese edition goes on the market.

概念；1989年GRiD公司推出第一款带有触摸式屏幕的便携电脑；1992年，微软推出了"Windows for Pen"，但两年后遭淘汰；1996年Palm研制出第一款成功应用的触笔式电脑；1997年Oracle加入微软的行列开始研究平板技术；2000年是平板电脑的重要转折点之一，比尔盖茨由2000年开始，连续两届Comdex大会上的讲话中大力倡导平板电脑；2002年，平板电脑发展史上最重要的一年，也是最忙碌的一年，该年5月，盖茨带着150台平板电脑参加微软年度CEO峰会，接着8月份，平板电脑软件向电脑制造商开放，紧接着在11月份，平板电脑正式上市，七大电脑制造商开发这一新产品，12月平板电脑中文版上市。

There is a small action range in archery.Simple methods and analysis is inadequate,so the author,on the basis of testing the archery athletes in national team with the Qualisisy infrared photoelectric testing system,Footscan sole pressure testing system,Noraxon myoelectricity remote testing system, etc,analyzes comprehensively the action technique of the athletes in national team from the posture,body motion characteristics,body posture equilibrium and stability,distribution of centre sole pressure, myoelectricity characteristics,etc,with the methods of relative software and data statistics.The conclusions and the suggestions are as follows:Conclusion:Firstly,the communality of posture angle,muscle strength,the activation order of main muscle during raising the bow, raising the bow and the timing of the raising could be used as the evaluation index of technique action in archery;Secondly,the length of the time needed in the different periods couldn\'t be used as technique evaluation index since it has no relation with the result.But the time of raising and pulling back the row is relatively fixed,and the time has a positive relation with the archery achievement of the national team in China.Thirdly,the central index of the sole pressure could be used as the reference criterion of selecting athletes and forecasting achievement.During playing archery,the interrelation between the central index of the sole pressure and the achievement is different individually and in different periods.Fourthly,there is low stability of the archery athletes in the front and back direction during the action, caused by the athletes\' posture disequilibrium of dorsal abdominal muscle and non-professional sport shoes..Fifthly,kinematics and electromyography shows that the people on archery in China haven\'t taken the raising and pulling back the row seriously.

射箭的肢体动作幅度小，简单的手段和分析不能满足其需要，笔者结合各种运动生物力学仪器优点及射箭项目的特性，选用Qualisisy红外光点测试系统、Footscan足底压力测试系统、Noraxon肌电遥测系统等对国家队射箭队运动员进行多方位的测试，运用相关软件及数理统计方法，对运动员的射箭动作技术，从射箭动作的姿态构架、肢体运动特点，身体姿态平衡稳定性、足底压力中心分布、肌电特性等进行全面分析，结论及建议如下：结论：第一，姿态角、肌肉用力激活程度、举弓阶段主要用力肌肉激活顺序、举弓、开弓时间等的一致性可作为射箭技术动作评价指标；第二，各动作阶段所用时间的长短不能作为技术评价指标，但举、开弓的时间相对较固定，开举弓时间比与中国国家队射箭成绩正弱相关达到显著性；第三，闭眼状态下足底压力中心单位面积轨迹长与144支箭最好成绩正相关达到显著性，可作为身体素质、选材及成绩预测的参考依据；实射时身体平衡的足底压力中心指标与成绩的相关程度具有个体性差异、阶段性差异；第四，射箭运动员实射时身体在前后方向上的稳定性较差，这与运动员的站立姿态、腹背肌力量的不匹配以及射箭没有专业运动鞋有关；第五，运动学及肌电学都表明中国射箭训练对举弓、开弓阶段的重视不够，背部肌肉用力特征不明显，撒放技术合理性不高。

Taking the plate tectonics as leading theories, combining outcrop, seismic techniques, drilling and logging datum, inter-verifing manifold researching methods, The paper has amply researched the region. discussed the coupling relation of basin-mountain and the geodynamics system; analyzed geologic tectonic characteristics of the basin; confirmed superposed alteration in Mesozoic of the basin; recompartmentalized tectonic units of the researching region and the basin; studied the tectonic evolution of the basin since Mesozoic; ascertained four large tectonic layers and five tectonic evolution process stages in different ways of formation and tectonic alteration; established different tectonic stages、compages、models and sedimentary filled processes, based on the organic geochemistry and oil-gas geologic characteristics in middle Cenozoic of the basin, the conclusions have been summarized in the paper, such as:The primary source rock in lower Permian and subordinate source rock in upper Triassic are developed in the region with better quality, and secondary hydrocarbon is possible; The bearing system which is comparted by three large tectonic layers and two unconformity surfaces formed in Palaeozoic、upper Palaeozoic to Triassic and Cenozoic developing in the region, compounded with reservoirs of different lithology is developed in the region, while the property of tectonic fracture reservoir is better; Based on the analyzing petroleum accumulation elements and preserving conditions, the deep depressing belt in the north of Luoyang depression、Shiling-Yiyang folded fault belt in Yiyang depression and wide anticline belt in Yichuan depression are advantageous petroliferous region, Zhaizhen anticline in Luoyang depression and Shimen wide anticline on footwall of overthrust in Yichuan depression are optimum tectonic traps.

本文以板块构造理论为指导思想，采用露头区野外地质与盆地地震、钻井、测井资料密切结合，多种测试方法互检的研究方法，探讨了盆山耦合关系及其所控制的地球动力学系统；分析了研究区域和盆地地质结构构造特征，确定了中生代盆地的叠合改造性质，并重新划分区域构造单元和盆地构造单元，深入研究了洛阳—伊川盆地自中生代以来的构造演化；确定了成盆作用和构造改造作用的四大构造层和五期不同方式、方向的构造演化过程；建立了不同构造阶段的构造组合和样式及与构造相关的沉积充填过程；在此基础上通过多种有机地球化学指标，研究盆地中新生代油气地质特征，认为本区发育下二叠统区域主力烃源岩、上三叠统区域非主力烃源岩，两套烃源岩质量均较好，并具备二次生烃条件；认为本区发育古生界、上古生界—三叠系和新生界三大构造层、两个不整合界面分隔、不同岩性储集层叠置组合和构造裂隙型储集层的复式储集体系，其中构造裂隙型储集层储集性能较好；依据油气成藏条件和保存条件分析，认为洛阳凹陷北部深凹带、宜阳凹陷内石陵—宜阳褶断带以及伊川凹陷伊川宽阔背斜带为较有利含油气区，有利的构造圈闭是洛阳凹陷翟镇背斜和伊川凹陷的石门逆冲断层下盘的宽缓背斜

The next Day after I came home to my Hutch with him, I began to consider where I should lodge him, and that I might do well for him, and yet be perfectly easy my self; I made a little Tent for him in the vacant Place between my two Fortifications, in the inside of the last, and in the outside of the first; and as there was a Door, or Entrance there into my Cave, I made a formal fram'd Door Case, and a Door to it of Boards, and set it up in the Passage, a little within the Entrance; and causing the Door to open on the inside, I barr'd it up in the Night, taking in my Ladders too; so that Friday could no way come at me in the inside of my innermost Wall, without making so much Noise in getting over, that it must needs waken me; for my first Wall had now a compleat Roof over it of long Poles, covering all my Tent, and leaning up to the side of the Hill, which was again laid cross with smaller Sticks instead of Laths, and then thatch'd over a great Thickness, with the Rice Straw, which was strong like Reeds; and at the Hole or Place which was left to go in or out by the Ladder, I had plac'd a kind of Trap-door, which if it had been attempted on the outside, would not have open'd at all, but would have fallen down, and made a great Noise; and as to Weapons, I took them all to my Side every Night.

应该说，我现在的裁缝手艺已相当不错了。另外，我又给了他一顶兔皮帽子，戴起来挺方便，样子也很时髦。现在，他的这身穿戴也还过得去了。他看到自己和主人几乎穿得一样好，心里十分高兴。说句实话，开始他刚穿上这些衣服时，深感行动不便；不但裤子穿起来感到很别扭，而且，背心的袖筒磨痛了他的肩膀和胳肢窝。后来我把那使他难受的地方略微放宽了一些，再加上对穿衣服也感到慢慢习惯了，他就喜欢上他的衣着了。回到家里第二天，我就考虑怎样安置星期五的问题。我又要让他住得好，又要保证自己绝对安全。为此，我在两道围墙之间的空地上，给他搭了一个小小的帐篷，也就是说，这小帐篷搭在内墙之外，外墙之内。在内墙上本来就有一个入口通进山洞。因此，我在入口处做了个门柜和一扇木板门。门是从里面开的。一到晚上，我就把门从里面闩上，同时把梯子也收了进来。这样，如果星期五想通过内墙来到我身边，就必然会弄出许多声响，也就一定会把我惊醒。

This paper tries to have some innovations in the following aspects:(1) the main cause of our tax revenue depending mainly on turnover tax is short of innovation in system;(2) its innovation in system to impel the rising of income tax in developed counties, which is a good example for us to learn from in the optimization of our tax structure ;(3) the main function of turnover and income tax should be separated according to their characters;(4) the reforms in turnover and income tax should be taken simultaneously, congenially and complementally, although the former will be more progressive while the later more abrupt;(5) the essential function of tax is to raise revenue, but its fair-promoting effect is much more obvious in disbursing than in collecting.

本文力求在以下几个方面有所创新：(1)认为我国税种收入过度依赖流转税的主要原因是制度创新力度不够；(2)认为制度创新是推动发达国家所得税从弱到强的主要原因，提出我国税种结构优化需要在制度上大胆创新；(3)提出根据各个税种的特性，流转税和所得税的主要功能应有所分工；(4)流转税和所得税的改革应双管齐下，以渐进的方式改革流转税，以突变的方式改革所得税，分工协作，优势互补；(5)提出税收的主要功能是筹集收入，税收在征收环节在促进结果公平方面效果甚微，税收在支出环节更能有效促进结果公平。

This paper tries to have some innovations in the following aspects:(1) the main cause of our tax revenue depending mainly on turnover tax is short of innovation in system;(2) it's innovation in system to impel the rising of income tax in developed counties, which is a good example for us to learn from in the optimization of our tax structure ;(3) the main function of turnover and income tax should be separated according to their characters;(4) the reforms in turnover and income tax should be taken simultaneously, congenially and complementally, although the former will be more progressive while the later more abrupt;(5) the essential function of tax is to raise revenue, but its fair-promoting effect is much more obvious in disbursing than in collecting.

本文力求在以下几个方面有所创新：(1)认为我国税种收入过度依赖流转税的主要原因是制度创新力度不够；(2)认为制度创新是推动发达国家所得税从弱到强的主要原因，提出我国税种结构优化需要在制度上大胆创新；(3)提出根据各个税种的特性，流转税和所得税的主要功能应有所分工；(4)流转税和所得税的改革应双管齐下，以渐进的方式改革流转税，以突变的方式改革所得税，分工协作，优势互补；(5)提出税收的主要功能是筹集收入，税收在征收环节在促进结果公平方面效果甚微，税收在支出环节更能有效促进结果公平。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。