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The thymidine kinas gene was cloned from human herpes simplex virus Ⅱ by PCR techniques; eukaryon expressing vector pcDNA3/tk and pcDNA3/angio/tk fusion gene was constructed by DNA recombination; The treating effects of human primary liver cancer strain SMMC-7721 in vitro and nude mouse model transplanted with human liver cancer in vivo are studied.

通过PCR技术，由人单纯疱疹病毒DNA克隆获得单纯疱疹病毒Ⅱ型胸苷激酶基因；通过基因工程技术，构建真核表达质粒pcDNA3/tk及融合基因真核表达质粒pcDNA3/angio/tk；研究其对人原发性肝癌SMMC-7721细胞株的体外杀伤作用及对人原发性肝癌裸小鼠移植瘤模型的治疗作用。

Plant lectin has important in vivo physiological functions, and it is necessary to expressing BanLec gene in Escherichia coli and investigate its functions.

植物凝集素具有重要的生理功能，通过原核表达香蕉凝集素基因并探讨其功能具有重要意义。

PartⅡExperimental Study on Acute Hypatocyte Necrosis and Apoptosis with MRI, DWI and MRS Objective: To demonstrate the ability and value of MR imaging, diffusion-weighted imaging, and MR spectroscopy in detecting and characterizing necrosis and apoptosis in vivo with a comparison to the pathology after establishment of hepatic necrosis and apoptosis animal model by different surgical ligations of hepatic blood supply vessels.

目的：通过手术结扎的方法建立大鼠肝细胞坏死和凋亡的动物模型，将不同时间点的MRI、DWI和MRS检测结果与病理学所见进行对照研究，以评价MRI、DWI及MRS在检测及鉴别肝细胞凋亡和坏死两种病理改变的能力与价值。

In vivo-induced genes spd_0300, spd_0414, spd_0622, spd_1663, spd_1719, spd_0235, spd_0873, spd_1672 regulated by transformation were screened out and they may participate in the processes such as growth regulation, temperature reception, carbohydrate metabolism, and lipoids metabolism.

筛选出受转化调控的体内诱导基因spd_0300、spd_0414、spd_0622、spd_1663、spd_1719、spd_0235、spd_0873、spd_1672，它们可能参与生长调节、温度感应、糖脂代谢等环节，表明细菌转化可通过调节某些体内诱导基因的表达来增强细菌的毒力。

Objective:The aim of the present study was to investigate the effects of Xipayiguyinye on oral bacteria and oral malodour in vitro and in vivo.It will provide references for the clinical application of Xipayiguyinye.

目的：本研究通过体外抑菌和体内试验，评价西帕依固龈液对口腔主要致臭菌抑菌作用及体内防治半胱氨酸激发性口臭的效果，为其进一步在临床应用提供依据。

The aim of the present review article is to focus on the results of study on the zeolites detoxifying mycotoxins in vitro and in vivo. The mechanisms and the problem of zeo...

本文重点综述了沸石脱除霉菌毒素的体内外实验结果，介绍了沸石消除霉菌毒素毒性的作用机理及存在的问题，提出了沸石作为霉菌毒素脱毒剂进行改性加工及相关产品集成组装的原则，旨在为开发研制沸石类高效霉菌毒素脱毒剂产品提供科学参考。

Objective To study the stability of self-assembled system of bolaamphiphilic prodrug of zidovudine in rabbit plasma, and predict its behavior in vivo.

摘要目的考察齐多夫定两亲性前药自组装体系在家兔血浆中的稳定性，预测其自组装体系的体内行为。

Objective To evaluate the effect of TILs treatme nt onpostoperative patients with hepatocellular carcinoma and the in vitr o and in vivo hepatic targeting tendency of a compound of Galactosyl-Anti-CD 3McAb-TILs.

目的 探讨肿瘤浸润性淋巴细胞对原发性肝癌患者术后细胞免疫功能的影响和临床疗效及半乳糖抗CD3单克隆抗体-TIL复合物（Gal-Anti-CD3-McAb-TIL体外及体内的趋肝性。

Purpose To compare the dynamic changes of CD4(superscript +)CD25(superscript +) thymocytes development in vivo and in vitro.

目的 比较研究小鼠体内外胸腺细胞CD4CD25双阳性T细胞发育的动态变化，为天然调节性T细胞的研究提供基础数据。

The in vivo adjuvant effect of PEI for DNA vaccines was examined in EL4/EG7-OVA syngeneic mouse lymphoma model.

在动物实验中，我们於小鼠脚掌经皮下/皮间的途径接种疫苗，发现接种PEI/DNA复合物疫苗的小鼠，其接种位置的树突状细胞会呈现OVA抗原并移行到近端淋巴结。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。