查询词典 in vivo

In addition, baculovirus are noninfectious to vertebrates, and their promoters have been shown to be inactive in mammalian cells. Futhermore, the expression level of some recombinant proteins is up to 1000 mg/L. Finally, recombinant proteins are soluble and antigenically, immunogenically and functionally similar to their authentic counterparts. In this article, we used the transfer vector pBlueBac of AcNPV (Autographa californica Nuclear Polyhedrosis Virus) to construct the recombinant baculovirus AcNPV-GM-CSF by in vivo recombinantion.

本文中，我们首先利用苜蓿夜蛾核型多角体病毒带β-Galactosidase 基因标记的非融合蛋白基因转移载体pBlueBac，通过细胞内同源重组的方式将hGM-CSF基因成功地插入病毒AcNPV的基因组中。hGM-CSF基因编码信号肽和完整的氨基酸序列。hGM-CSF基因在感染重组病毒的草地夜蛾培养细胞Sf9中得到表达，感染后的Sf9细胞培养液能刺激人骨髓细胞在体外形成典型的集落，表达水平可达2.7〓CFU／ml。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

This point can reasonably account for the failure of directly oxidative radioiodination of alkaloids observed in our lab and in other labs over the world. The in vivo biodistribution and pharmacokinetics of -iodo-α-Allocryptopine and -iodo-chelidonine were studied in normal mice.

这一机理可较好地解释为何难于通过直接氧化法对生物碱化合物进行放射性碘标记研究了碘-131标记的α-别隐品碱和白屈菜碱在正常小鼠体内的组织分布和代谢动力学。

Lupeol, a pentacyclic triterpene present in mango, is a biologically active compound that has been reported to possess a number of pharmacological properties in the in vivo and in vitro studies.

羽扇豆醇是芒果所含的五环三萜，据报道，羽扇豆醇是一类生物活性物质，在体内及体外研究中均表现出多种药理特性。

Results In vitro recovery of the probe was mainly influenced by the perfusion rate. When the perfusion rate was set at 1.0μL/min, in vitro and in vivo recoveries of the probe were 50.16±2.34% and 14.096±0.58%, respectively. The enhancement indexes for 3%, 5% mentha oil were 3.8 and 17.6, respectively. The enhancement indexes were 9.2 and 24.8, for 3% and 5% of Azone, respectively. Conclusion The steady sate flux of meloxicam in rats is significantly improved by enhancer mentha oil and azone.

结果： 在实验条件下微渗析探针的体外回收率主要受灌注液流速的影响，为（50.16±2.34）%；反向渗析法测得探针的在体回收率为（14.096±0.58）%；MO的体积分数为3%和5%时，增渗倍数分别为3.8，17.6；azone的体积分数为3%和5%，增渗倍数分别为9.2，24.8；结论：不同体积分数的MO和azone均可显著增加美洛昔康经皮渗透稳态流量，azone的体积分数为5%时，大鼠角质层的屏障作用可能受到了较大程度的破坏。

MPP is what really works.Many in vivo studies have proved MPTP can cause PD.We are interested in investigating the influence of MPP+ on mesencephalic Dopamine neurons in vitro and to make sure whether it can be used as an PD model in vitro.

本实验研究拟通过MPP~+对体外培养的中脑多巴胺能神经元的影响的观察探讨MPP~+致PD发病的可能机制以及用制备MPP~+体外的PD模型的可能及其价值。

In addition, the influences of BIS on the activities of lymphocytes in vivo and in vitro were studied, including the blast transformation of lymphocytes, the number of acid α-naphthyl acetate esterase positive lymphocyes, the cytostatic effect of hepatic LGL against hepatoma cells, in older to provided the most adequate scientific foundation for the best application of hepatoma immunotherapy.

此外还对上述不同免疫制剂在体应用对正常大鼠血淋巴细胞转化率及酸性非特异性酯酶阳性淋巴细胞数量的影响；不同免疫制剂对分离培养的肝大颗粒淋巴细胞抑肝癌细胞增殖的直接影响的研究，为肝癌免疫治疗的更佳选择提供更充分的科学依据。

The best quality of MRS is that the MRS is the only noninvasive and in vivo means in detecting the changes in metabolism state and special biochemical compounds in living animal and human body, and that make it possible for us to observe the physiological and biochemical characteristic of brain neoplasma ,and the development of neoplasma.

脑肿瘤是较为常见的肿瘤，其发病率占全身肿瘤的1.8％，其中以脑胶质瘤为最多，占43.7％，脑肿瘤的诊断及治疗一直是临床及基础研究的难点及热点，为了更好的了解胶质瘤的生物学特征，寻找有效的治疗方法，人们一直在寻求建立稳定安全的脑动物模型，大鼠C6胶质瘤具有与人脑胶质瘤相似的生物学特征，且模型较为稳定，被广泛用于肿瘤生物学及实验性治疗的研究中。

In separate in vivo experiments, perinatal asphyxia was induced 4 hours after preconditioning with analgesic doses alone and in combination; infarct size was assessed 7 days later, and neuromotor function was evaluated at 30 days in separate cohorts.

在单独的体内实验中，围产期窒息通过4小时后预处理单独和组合镇痛剂量诱导后；7天之后评估梗死面积，在30天内的单独评价神经功能。

Presently, the studies on multidrug resistance mechanism, reversor and associated exterior experiments in vivo and in vitro from different points of view and levels show that multidrug resistance is the result of common action in many factors. In recent years nonrepresentative mechanism of multidrug resistance becomes more and more significant.

肿瘤细胞产生耐药性成为许多肿瘤治疗成败的关键，也是血液肿瘤化疗失败的重要原因，目前人们从不同的角度和水平对耐药的发生机制、逆转耐药的药物研究及相应的体内外实验等进行研究，指出多药耐药是多种因素共同作用的结果。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。