查询词典 in vitro

The recombinant chimeric antibody ch-BDI against human bladder tumor show fairly good function both in vitro and in vivo.

所表达嵌合抗体ch-BDI在体内外有良好针对人膀胱癌的特异性结合活性，具有潜在的临床应用前景。

Methods Determination of DNJ by derivatization with 9-fluorenylmethyl chloroformate and followed by reversed-phase high-performance liquid chromatography and a fluorescence detector; the inhibitory curves on α-glucosidase at the enzymatic level and the activities to α-aglucosidase in vitro were observed in the everted sac experiment.

以氯甲酸药甲酯柱前衍生RP-HPLC荧光检测法对DNJ进行分析；在酶水平测定样品的α-糖苷酶抑制曲线，在离体水平用反转肠囊实验测定样品的α-糖苷酶抑制活性。

In addition, LQ of neural stem cells can be induced in vitro to differentiate into cholinergic neurons.

另外，LQ可以诱导神经干细胞体外定向分化为胆碱能神经元。

OBJECTIVE: After purification and amplification in vitro, the bone marrow mesenchymal stem cells were induced into chondrocytes, and then implanted them at the bottom of nano-hydroxyapatie/chitosan scaffold for combined culture. The aim was to investigate the feasibility of them used in tissue-engineered chondral composite graft.

目的：将体外提纯、扩增的骨髓间充质干细胞诱导成软骨细胞，将其接种于穿&靴&的纳米羟基磷灰石/壳聚糖支架的底部上联合培养，探索其用于组织工程软骨复合体的可行性。

This experiment approach rat-MSCs which are fractional cultivation、generation in vitro, and in Chinese crude drug circumstance to derivationrat-MSCs chondroblast differentiated, to explore the front view ofcartilage coloboma architecture engineering recovery by Chinese crudedrug.

本实验探讨大鼠骨髓间充质干细胞在体外分离培养、增殖，以及在中药环境下诱导向成软骨细胞定向分化，探索软骨缺损组织工程修复中的中药应用前景。

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于：（1）通过骨髓基质干细胞的原代分离、培养及成软骨诱导，观察原代细胞扩增能力及其向成软骨方向分化的潜能；（2）寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件；（3）观察经成软骨诱导的MSC在PDLLA、PDLLA／壳聚糖多孔载体上三维的增殖状况，探求细胞与材料间的相互作用；（4）观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况，了解维持诱导细胞软骨表型的合适条件及作用机制。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Methods Autologous BMMSC of New Zealand rabbits were acquired and induced into chondrogenic differentiation by adding transforming growth factor-β1 (TGF-β1), dexamethasane and ascorbic acid in vitro. Then the chondrocytes were implanted onto novel polymeric scaffolds made by Poly (dl-lactide-co-glycolide) embedding with chitosan nonwoven cloth. The tissue engineering cartilaginous grafts composed of chondrogenetic BMMSC/scaffolds were finally transplanted into the defects of ear cartilage in rabbits of the experimental group.

取新西兰大白兔BMMSC体外培养扩增，以含转化生长因子(TGF-β1)、地塞米松和维生素C的培养液做诱导培养，实验组以诱导后的软骨细胞与聚丙交酯－乙交酯共聚物Poly(dl-lactide-co-glycolide)包埋甲壳胺无纺布的新型支架形成复合物修复兔自体耳廓软骨缺损；对照组以PLGA/甲壳胺无纺布支架修复兔耳廓软骨缺损。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

ES细胞为研究基因功能、胚胎发生和个体发育，及组织细胞分化机理提供了理想的模型。近年来的研究表明，适当条件下，ES细胞可定向分化为三个胚层的各种类型的细胞，其中包括向神经元、造血细胞、成骨细胞、心肌细胞、血管内皮、软骨细胞、平滑肌特方向的定向分化。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

但定向分化为表皮干细胞至今未见报道。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。