查询词典 in vitro

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

Methods: The bone marrow cells were obtained from long bone of new born Zelanian rabbits, the cells were isolated and cultured in vitro in conditioned medium, then the acquired cells which had propagated in vitro and undergone frozen storage and resuscitation and gelatin sponge were made into a complex.

取新生新西兰大白兔四肢长管骨骨髓，分离后加入条件培养液体外培养，冻存复苏的细胞以明胶海绵吸附后回植于异体兔的桡骨缺损处作为实验侧，对侧以明胶海绵吸附生理盐水作为对照侧。

DNA could be damnified by the NOCs in tap water directly, and the DNA adducts of such NOCs were formed in vitro and in vitro test.

东湖自来水中的NOCs有直接损伤生物体内外DNA、形成DNA加合物的作用。

By examining their expression during in vitro decidualization, we found that timing of the DAPs' expression in vitro was not consistent with it in vivo.

分析它们在体外蜕膜化过程中的表现情形，发现与在体内蜕膜化过程中的表现不一致。

This study was carried out to investigate whether the GV oocytes from carcasses could be utilized. Carcasses were preserved at 10℃, 15℃, 20℃ and 25℃ for 8 hours, 14 hours, 24 hours, or 48 hours after mice were killed and GV occytes were collected. After maturating in vitro, these oocytes were fertilized in vitro with the general procedure in advance.

将ICR系雌性小鼠处死并在10℃、15℃、20℃和25℃下依次保存8、14、24和48h后，采集其体内的卵巢GV期卵，采用常规方法进行体外成熟和体外受精，获得的2细胞期胚经体外培养或胚胎移植观察其发育能力。

To study the anti-tumor activity of Masson's pine bark extract in vitro and in vivo, MTT assay was used to observe the growth inhibitory effect of MPBE on human colonic cancer cell line VoLo in vitro.

研究马尾松树皮提取物体外和体内的抗肿瘤作用。

In this paper, three two-year old Xinjiang Kazak wethers fitted with permanent ruminal cannulas were used as rumen liquor donors. Three experimental serials were conducted:Serial Ⅰ: Studies on gas production technique in vitro A gas production apparatus has been designed to study on maize straw fermentation in vitro.

本论文以三只安装有永久性瘤胃瘘管的新疆哈萨克二岁羯羊为瘤胃液固定供给动物，进行了三个系列的试验研究：试验系列Ⅰ：体外产气技术的研究。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。