查询词典 in vitro

Five of fifty random primers amplified DNA polymorphism hands in RAPD analysis of seed-derived seedlings and of in vitro regenerated plantlets taken at random. One primer eyen amplified the difference between the regenerated plants. This suggested that there was some variation in DNA in plants of M. sativa regenerated in vitro.

随机选取实生苗和下胚轴愈伤组织再生苗进行RAPD分析，结果表明，所用的50个随机引物中有5个扩增出差异性条带，甚至再生植株之间也有1条引物扩增的条带有差异，这说明经组织培养所得到的再生植株与实生苗相比，在DNA水平上发生了一定程度变异，并且这种变异也存在于再生植株之间。

To further investigate their roles in cell proliferation apoptosis and differentiation, three human leukemia cell lines (K562, U937 and HL60) exposed to various concentration of hexamethylene bisacetamide, a potent inducer of differentiation of transformed cells both in vivo and in vitro, were used as an in vitro model for the study of cell proliferation, differentiation and apoptosis.

为了进一步研究cyclin A和p21在白血病细胞增殖，分化，凋亡中的作用，我们用在体内，外均有抗肿瘤作用的药物六亚甲基二乙酰胺在体外抑制白血病细胞株K562，U937和HL60增殖，诱导其分化，成功地建立了与药物干预浓度呈剂量-效应关系的，具有不同增殖，分化，凋亡状况的实验细胞模型。

Objective To observe the development of mouse kidney in vitro at embryonic day 12、14、16 and postnatal day 1, and compare with the development of homeochronous kidney in vivo. And to select eligible the embryonic age for kidney culture in vitro.

目的 观察胚龄12、14、16、以及生后1天的肾组织体外培养的发育情况，与同期在体发育进行比较，探讨肾组织体外培养的胚龄选择。

Methods The in vitro cultured porcine vascular endothelial cells and human sera were co-reacted to set up an in vitro model of hyperacute rejection of pig-to-human xenotransplantation.

用体外培养的猪血管内皮细胞和不同人的血清共同反应，建立猪与人异种移植超急性排斥反应的体外实验模型。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

The mice were used as experimental animals in this study,through the experiment in vitro and in vivo,systemic invested the dynamic changes in expression and localization of FGF7,FGF10 and its receptor in mammals development,lactation and involution;revealed the relationships between the expression of FGF7,FGF10 and its receptor and the function;clarified the function of FGF7 and FGF10 in mammary gland development,lactation and involution,and the effect of mammogenic hormones on expression of FGF7 and FGF-10 and its receptor in different periods in vitro.

本研究主要以小鼠为实验材料，通过体外和体内实验，系统研究FGF7、FGF10及KGFR在哺乳动物乳腺发育、泌乳及退化过程中表达定位的动态变化，揭示FGF7、FGF10及KGFR表达变化与乳腺发育及泌乳功能间的对应关系，阐明FGF7、FGF10在乳腺发育、泌乳及退化过程中的功能以及乳腺发育激素对不同时期乳腺FGF7、FGF10及KGFR表达的影响。

Results In vitro recovery of the probe was mainly influenced by the perfusion rate. When the perfusion rate was set at 1.0μL/min, in vitro and in vivo recoveries of the probe were 50.16±2.34% and 14.096±0.58%, respectively. The enhancement indexes for 3%, 5% mentha oil were 3.8 and 17.6, respectively. The enhancement indexes were 9.2 and 24.8, for 3% and 5% of Azone, respectively. Conclusion The steady sate flux of meloxicam in rats is significantly improved by enhancer mentha oil and azone.

结果： 在实验条件下微渗析探针的体外回收率主要受灌注液流速的影响，为（50.16±2.34）%；反向渗析法测得探针的在体回收率为（14.096±0.58）%；MO的体积分数为3%和5%时，增渗倍数分别为3.8，17.6；azone的体积分数为3%和5%，增渗倍数分别为9.2，24.8；结论：不同体积分数的MO和azone均可显著增加美洛昔康经皮渗透稳态流量，azone的体积分数为5%时，大鼠角质层的屏障作用可能受到了较大程度的破坏。

MPP is what really works.Many in vivo studies have proved MPTP can cause PD.We are interested in investigating the influence of MPP+ on mesencephalic Dopamine neurons in vitro and to make sure whether it can be used as an PD model in vitro.

本实验研究拟通过MPP~+对体外培养的中脑多巴胺能神经元的影响的观察探讨MPP~+致PD发病的可能机制以及用制备MPP~+体外的PD模型的可能及其价值。

In trial 1, the in vitro gas production, in vitro organic matter digestibility and metabolizable energy of rice straw block Ⅰ,Ⅱ,Ⅲ,Ⅳ and alfalfa block were measured.

试验1，通过体外产气法测定了稻草块Ⅰ、Ⅱ、Ⅲ、Ⅳ和苜蓿草块的体外产气量、产气参数、体外有机物消化率及代谢能。

Abstract］objectiveto observe antiseptic effect and bacteriostatic concentration in vitro of tieshanxuanyangping.methodsto determine bacteriostatic concentration in vitro for staphylococcus, bacillus coli, red trichophyton, purple trichophyton, trichophyton mentagrophytes, microsporum gypsen with doubling dilution.

摘要］目的观察&铁扇癣痒平&体外抗菌效果和抑菌浓度。方法采用对倍液体稀释法测定该药对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌、须毛癣菌和石膏样小孢子菌体外抑制作用。

Alternatively, the con- trollers can use the synchronous rectifier itself or loss- less inductor current-sensing methods to provide overload protection with lower power dissipation.

另外，康威特罗勒斯可以使用同步整流器本身或亏损减少电感电流检测方法，以提供低功耗过载保护。

Mr. Dauber's other Schatz hangs in his home movie studio.

多伯把沙兹的另一件作品挂在家里的电影室。