查询词典 in vitro

Along with rapid development of bio-technology, artificial chromosome doubling in vitro is the efficiency approach of producing polyploidy plants. Advances on in vitro induction of autotetraploid in plants was reviewed.

随着生物技术的迅速发展，通过植物离体组织人工诱导多倍体已经成为获得多倍体植株的有效途径，本文就植物离体组织染色体加倍诱导同源四倍体的研究进展做一介绍。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer .

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

In vitro experiment results demonstrated that the decrease in reproductive activity in vitro of BMSCs of osteoporosis rats contributes to osteoporotic cytopathology.

体外实验结果表明，骨质疏松大鼠骨髓间充质干细胞的体外增殖能力下降可能是骨质疏松的细胞病理学机制。

Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果："长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用，量效关系明显："长动"与放疗和化疗分别联合应用有明显的增效作用：作用机制研究结果：细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用，量效关系明显；免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖，增强NK细胞的活性，对IL-2的产生无明显影响；"长动"在体外可明显诱导人体肝癌QGY细胞凋亡，使细胞阻滞于G0-G1期。

The infectivity of TMV recovered by dialysis from incubation with anthraquinone glycosides in vitro has been reduced in contrast to infectivity of TMV control, it seems that anthraquinone glycosides have direct viricidal effect on TMV. The anthraquinone glycosides may interfere recognization of TMV to host receptor when applied before TMV inoculation. The anthraquinone glycosides can inhibit multiplication of TMV in infected leaf discs but did not inhibit infectivity of TMV-RNA in vitro. The control of tobacco mosaic virus disease of N.

总蒽醌甙对TMV混合，对粒体形态无破坏作用，但经透析，其抑制作用仍较高，表明总蒽醌甙与TMV结合能力强不易解离或对粒体具有直接毒害作用；总蒽醌甙预先接种可能影响病毒对侵染位点的识别，对叶圆片中的病毒具有抑制复制的作用，但在体外混合不影响TMV-RNA的侵染活性，可能与总蒽醌甙干扰病毒的衣壳装配过程或影响复制酶的活性有关。

Ray florets elongated faster than disc florets from phase P3, when tetrads of haploid microspores appeared, and the pigmentation of the ray floret began when the pollen grains showed germination apertures in the disc florets of the first whorl. The effects of light, sugar and GA3 on flower growth and development were investigated in vitro. Inflorescences in the P2 phase or later could grow and bloom in vitro, and sucrose, GA3 and light promoted flowering.

研究表明，分化完全的舌状花在P3期、即花粉细胞四分体出现之后长度超过盘状花，在盘状花的雄蕊出现具有萌发孔的花粉粒之后，舌状花开始着色。P2以后的花序经离体培养可以开放，光、糖和GA3对花序的生长和开放有显著的促进作用。

In the experiment, the tea plant shoots were cultured in vitro, and inoculated artificially. The result showed the disease condition in vitro was basically consistent with that of the tea plantation in nature. Therefore, we thought this identification method of tea plant resistance to tea brown blight was feasible, especially a large number of cultivars.

本试验采用离体插枝培养法，人为造成伤口、人工接种并保湿对茶树品种进行抗性鉴定，室内人工接种与田间自然发病的情况基本一致，因此，可以认为此方法对茶树品种抗云纹叶枯病鉴定是可行的。

But it in 10"8mol/L-10"mol/L inhibited gastrin secretion .whereas it promoted somatostatin secretion of duodenum in vitro. GnRH-A did not effect significantly gastrin and somatostatin secretion of stomach in vitro.These results suggested that GnRH-A may integrate with GnRH receptor and restrain directely gastric acid secretion in parietal cell.

综上所述，我们认为胃腔内的Gn尺H类似物可能首先和壁细胞上GnRE受体结合，直接抑制壁细胞胃酸的分泌，由于胃酸分泌减少反馈性地刺激胃泌素细胞，促进其合成及分泌胃泌素，胃酸分泌减少同时也反馈性的抑制生长抑素的释放，反馈性地抑制胃肠壁内EC细胞分泌5一HT。

Inseminated in 8-9 hrs after GVBD, the ova have the highest hatching rate (more than 86% in the concerned spawn).Based on the above insemination research of the oocyte maturation in vitro, recombinant opAFP-pUC19 was microinjected into the germinal vesicle of oocyte of maturation in vitro.

经过卵母细胞时期在生发泡中注射外源抗冻蛋白基因的体外成熟卵，其受精率（70～80%）和孵化率（38～75%）均低于对照组10～30%，在进行外源抗冻蛋白基因注射后授精孵出的小鱼长至半成体和成体鱼后，分别用斑点杂交和Southern blo-tting分析外源基因的整合。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。