查询词典 in vitro

In this paper the effects of mercury chloride on the coefficient of visceral organ of mouse and the meiotic maturation and the fertilization ability of mouse oocyte were studied by in vitro culture and in vitro fertilization of mouse oocytes,etc.

为探讨汞化合物对脏器及生殖的毒性，采用卵母细胞体外培养、体外受精的方法研究了汞对小鼠脏器系数、卵母细胞成熟和受精能力的影响。

Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.

结果：与无上皮细胞相比，共培养明显促进了小鼠桑椹胚率（71.3%和61.2%，p.05），囊胚率（50.0%和39.7%，p.05），囊胚孵化率（34.8%和24.3%，p.05）和胚胎总细胞数（44.1和35.6，p.05）。结论：子宫内膜上皮细胞能够促进小鼠胚胎的体外发育，对早期胚胎的体外发育环境具有优化作用。

In this thesis, ribonuclease A was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. On this basis, the refolding mechanism in vitro was discussed.

本文以核糖核酸酶A为蛋白质体外重折叠的模型蛋白，对其体外重折叠过程进行了研究，探讨了这类目标蛋白复性过程的一些规律性的问题。

PDLSCs and SCAP grew very well on the scaffold of HA/TCP in vitro. Conclusion:The miniature pig PDLSCs and SCAP, which can be cultured successfully in vitro, may be useful cell sources for the regeneration of periodontal and tooth tissues.

小型猪牙周膜干细胞及根尖牙乳头干细胞可被成功的分离培养，在体外表现出良好的生长状态，有望为牙周组织再生和生物牙根再生提供有用的细胞来源。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

The in vitro releasing property of the preparation was examined by the method of in vitro delivery. The in vivo releasing property of the preparation was evaluated by the shadowgraph technique of barium sulfate. Results The preparation method of the PYTCSD was obtained.

以苦参碱和氧化苦参碱体外释放度为指标，对制剂的包衣处方进行筛选；采用体外释放度测定法考察该制剂的体外释放性能；采用硫酸钡造影技术对该制剂在人体内的释放性能进行评价。

This dissertation studied the isolation, purification, culture system optimization and transplantation techniques of goat spermatogonium in vitro respectively to construct an optimized long term culture system to facilitate in vitro manipulate studies for future.

十年来小鼠精原干细胞培养和移植探索的不断完善，为各种哺乳动物及人类精原干细胞培养体系的建立和移植实验提供了新的思路和技术平台。

This method is more convenient, rapid and economical than the traditional Sevag method. A standard strain of herpes simplex virus type 1(HSV-1, SM44 strain) was used to investigate the antiviral activities of PSP in vitro and in vivo. In vitro experiments were carried out in Vero cells, firstly the activity of PSP against HSV-1 was proved to be better than acidovir, which is the most widely used antiviral agent in clinic.

抗病毒作用的研究选用单纯疱疹病毒1型(HSV-1)标准株(SM44)，体外实验在Vero细胞中进行，在证实PSP抗HSV-1活性优于目前临床治疗该病毒感染的首选药物阿昔洛韦的基础上，进一步探讨了PSP抗HSV-1作用的机制。

In the present experiment, the screening techniques for salt tolerance and mutants of strawberry leaf and anther callus in vitro were studied, and primary screening system for salt tolerance evaluation was established. It was studied the sensitivity to mutagen of different strawberry cultivars and different tissues in vitro.

本试验以草莓离体叶片及花药愈伤组织为试材进行了耐盐突变体的诱变及筛选研究，初步建立了草莓耐盐突变体诱变筛选的技术体系，研究了草莓品种以及离体组织对诱变剂的敏感性，并且对不同诱变处理再生植株的耐盐性差异进行了比较。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1）叶片对辐射敏感，叶片再生的半致死剂量为10 Gy-20 Gy，'丰香'品种比'章姬'品种敏感； 2）辐射后8 d-15 d接种，'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势，而其他剂量则几乎未见有变化，'章姬'在剂量10 Gy-30 Gy，叶片的不定芽再生率提高较大，40 Gy仍表现轻微下降； 3）适宜的辐射剂量对草莓试管苗增殖有促进作用。

Alternatively, the con- trollers can use the synchronous rectifier itself or loss- less inductor current-sensing methods to provide overload protection with lower power dissipation.

另外，康威特罗勒斯可以使用同步整流器本身或亏损减少电感电流检测方法，以提供低功耗过载保护。

Mr. Dauber's other Schatz hangs in his home movie studio.

多伯把沙兹的另一件作品挂在家里的电影室。