查询词典 in vitro

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

By statistic analysis, there were significant differences with the average cell number of each morulae, early blastula and blastula between in vivo and in vitro, there were no differences with the hatching of blastula between in vivo and in vitro.

经统计分析体内桑椹胚、早期囊胚、囊胚的每一胚胎平均细胞数与体外桑椹胚、早期囊胚、囊胚的每一胚胎平均细胞数有显著差异；扩张囊胚的体内，体外每一胚胎平均细胞数没有显著差异。

The data suggest that Huayusanjie Tablet can prevent the formation and development of PVR in vitro and in vivo. Its mechanism concern with factors described below: lowering the concentration of PDGF, decreasing the expression of ICAM-1 and VCAM-1 in proliferative membrane and vascular endothelial cell, directly inhibiting proliferation of RPE in vitro.

化瘀散结片能有效防止PVR的形成与发展，其作用机理与减少玻璃体中血小板源性生长因子浓度，降低视网膜增生膜上和血管内皮细胞表面ICAM-1、VCAM-1的表达，以及直接抑制体外培养的视网膜色素上皮细胞增生有关。

Objective To compare serum-free medium AIMV with standard serum-containing medium in culturing IL-2-activated natural killer cells in vitro.Methods Proliferation,cytotoxicity in vitro and expression of CD11 c of the cells cultured in the two different media were compared.To observe the tumor target cells killed by adˉherent natural killer cellsthrough electroscope.

目的 研究方面比较无血清培养基AIMV与完全培养基对体外细胞因子激活的NK细胞的支持作用方法分别用AIMV及完全培养基培养粘附NK细胞和非粘附NK细胞，比较细胞的增殖能力、杀伤肿瘤细胞的能力、表达粘附分子CD11 c 的能力，并通过电镜对A-NK细胞所杀伤的肿瘤细胞进行形态学观察。

METHODS AND RESULTS: Adherent platelets express substantial amounts of SDF-1 and recruit CD34+ cells in vitro and in vivo. A monoclonal antibody to SDF-1 or to its counterreceptor, CXCR4, inhibits stem cell adhesion on adherent platelets under high arterial shear in vitro and after carotid ligation in mice, as determined by intravital fluorescence microscopy.

方法和结果：在体和离体的粘附血小板表达大量SDF-1、募集CD34+细胞，在体荧光镜下可见，在小鼠颈动脉结扎后、离体条件下的动脉内高剪切应力时，SDF-1或其相应受体的单克隆抗体－CXCR4抑制干细胞粘附于血小板上。

In this thesis, ribonuclease A was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro. On this basis, the refolding mechanism in vitro was discussed.

本文以核糖核酸酶A为蛋白质体外重折叠的模型蛋白，对其体外重折叠过程进行了研究，探讨了这类目标蛋白复性过程的一些规律性的问题。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中，与血清浓度为0的浓度组相比，与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少，且随血清浓度的增加，小鼠骨髓cfu-e和bfu-e的集落数逐渐减少，各浓度组间差异有显著性（p＜0.05）。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成，随患者血清浓度的增加，抑制作用增强，具有剂量依赖性。

Methods Both in vitro and in vivo models of ventilator-induced lung injury were used. Alveolar epithelial cells were stretched in vitro to mimic the lung injury in VILI. Rats were ventilated at large tidal volume to produce ventilator-induced lung injury in vivo. A total of 23 inflammatory cytokines were screened with micro gene array in stretched alveolar epithelial cells.

方法通过呼吸机诱导肺损伤的体内及体外模型，将分离的肺泡上皮细胞于体外拉长，利用基因微阵列技术分析并筛选出上调的炎性细胞因子，之后给予大鼠呼吸机机械通气，留取血清标本，测量这些细胞因子在血清中是否上调。

The inoculation of MDV in general indued to decrease the production of IL-2、 IFN-γ-Th〓 cytokines in vitro, and there are differences between spleen and Thymus lymphocyte on the production of IL-2 in vitro, only thymus lymphocyte IL-2 prodution increased transient in early time. The cytotoxity TNF induced in thymus and spleen lymphocyte and bone marrow macrophage shown a simillar curve of"V"shape. MDV infection cause cell-mediated immune declines.

MDV接种总体上降低IL-2和IFN-γ-Th〓细胞因子体外诱生，其中IL-2体外诱生在胸腺细胞和脾淋巴细胞有差别，前者有早期短暂增强作用；而对TNF在上述两种细胞和骨髓巨噬细胞的诱生作用呈现类似规律，即呈现&V&形曲线，在接种后早期（1~5天）和25天升高，而在10天开始下降，至第20天降至低谷，MDV感染导致雏鸡细胞免疫水平下降，促进MD发病。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。