查询词典 in vitro

Objective: To study osteogenic capability of rabbit bone marrow stromal cells and biological characteristical identification in vitro in order to explore the appropriate methods of osteoblast culture in vitro and to select an idea source of seed cells for bone tissue engineer.

目的：研究兔骨髓基质细胞在体外培养条件下的成骨能力及生物学特性，探讨简便易行的成骨细胞体外培养方法，为组织工程选择理想的种子细胞来源。方法：取新西兰白兔骨髓液经离心后得骨髓单个核细胞，以1× 106／ml的细胞浓度进行培养，经传代培养，选择条件培养液培养(1640完全培养液中加入地塞米松10-8Smol／l，β-甘油磷酸钠10mmol／l，VitC 50μg／ml)作为骨髓基质细胞增殖和成骨的调节因子，通过倒置显微镜、HE染色、扫描电镜、碱磷酸酶(alkaline phosphatase，ALP)测定、Ⅰ型胶原免疫组化染色和四环素荧光钙染色等手段对获得的细胞进行生物学特性研究。

The human TRIM5α-chimaera was expressed successfully in vitro, and the study demonstrates that the human TRIM5α-chimaera interacts with HIV-1 gag in vitro.

本实验在大肠杆菌表达系统中成功表达了重组Trim5α H(R328-332)蛋白，并且证实其在体外与HIV-1gag有结合作用。

Shown by GL-03 culture in vitro test, the ferment cultured milk has strong acid and cholate resistant ability, good adhesive, obvious inhibit to common pathogenic entero becteria, without drug tolerance factors, is a lactobacillus applicable to permanent planting of human body intestinal tract, and simultaneously the deterioration ratio of the cholesterol in vitro can be up to 56 percent; proved by the animal-test, the cholesterol-lowering cultured milk has the function of lowering serum cholesterol.

乳酸菌GL-03体外培养试验表明其有很强的耐酸、耐胆盐能力，粘附性好，对常见的肠道致病菌有明显的抑制作用，不携带耐药性因子，是一种适宜于人体肠道定植的乳酸菌，同时体外胆固醇降解率可达56％。动物试验证明降胆固醇酸乳具有降低血清胆固醇的功能。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

At the same time,the effect of injections such as Traditional Chinese Medicine injection of danshen and huangqi on the HPMC in vitro from patients with high coagulaˉtive state was investigated.Methods HPMCs from these patients and normal persons were sored by density gradient Fieoll-Hypaque and cultured48hours in vitro.PCA and PA from these patients and mormal persoms were examined by assay of chromophorous substrate.

分离高凝状态患者及正常人外周血单核细胞，短期培养后用发色底物法检测其促凝血活性和纤溶活性，检测加丹参注射液及黄芪注射液对体外培养HPMC表达PCA和PA的作用。

In vitro experiment results demonstrated that the decrease in reproductive activity in vitro of BMSCs of osteoporosis rats contributes to osteoporotic cytopathology.

体外实验结果表明，骨质疏松大鼠骨髓间充质干细胞的体外增殖能力下降可能是骨质疏松的细胞病理学机制。

The data suggest that Huayusanjie Tablet can prevent the formation and development of PVR in vitro and in vivo. Its mechanism concern with factors described below: lowering the concentration of PDGF, decreasing the expression of ICAM-1 and VCAM-1 in proliferative membrane and vascular endothelial cell, directly inhibiting proliferation of RPE in vitro.

化瘀散结片能有效防止PVR的形成与发展，其作用机理与减少玻璃体中血小板源性生长因子浓度，降低视网膜增生膜上和血管内皮细胞表面ICAM-1、VCAM-1的表达，以及直接抑制体外培养的视网膜色素上皮细胞增生有关。

During denuded mice oocyte mature process in vitro, the cortical granulesmay have abnormal numbers or generation problems. This may be another reason thatmakes denude oocytes matured in vitro have a clearly deficiency on fertilization invitro.

3去除卵丘细胞的小鼠卵母细胞在体外成熟过程中，皮质颗粒可能存在继发性生成障碍或者数量异常，该结果可能是去除卵丘细胞的小鼠卵母细胞存在体外受精障碍的原因之一。

Alternatively, the con- trollers can use the synchronous rectifier itself or loss- less inductor current-sensing methods to provide overload protection with lower power dissipation.

另外，康威特罗勒斯可以使用同步整流器本身或亏损减少电感电流检测方法，以提供低功耗过载保护。

Mr. Dauber's other Schatz hangs in his home movie studio.

多伯把沙兹的另一件作品挂在家里的电影室。