查询词典 in vitro

Based on the aim to make up the impercipience of NO in DN, the role of NO in DN was studied in some depth here through three different ways, i.e. in vitro, in vivo, and the Mendels genetic means.

为了有助于弥补人们当前对NO与DN认识的不足，本课题拟从体外、体内实验和群体遗传学三个角度对NO在DN中的作用机理进行深入研究。

Methods In vitro, human gastric cancer cell line SGC-7901 in log phase was intervened by As_2O_3 (final concentrations in order:1.0,5.0,10.0μmol/L) and 5-Fu (final concentration:50mg/L) respectively alone or in combination, and incubated for 24h、 48h、 72h. Blank group is set up. The cell apoptosis and cell cycle changes were observed by flow cytometry, and the expression of apoptosis-related gene Bcl-2 was analyzed by immunocytochemical method and absolute gray scales quantitative method.

将对数生长期的人胃癌细胞株SGC-7901(终浓度为2×10~5)经低、中、高三种浓度的As_2O(终浓度分别为1.0，5.0，10.0μmol/L)和5-Fu(终浓度50mg/L)单独及联合处理，设空白对照组，分别孵育24h、48h、72h后，收取标本采用流式细胞术测定细胞凋亡率及细胞周期变化；免疫组化绝对灰度定量分析检测凋亡抑制基因Bcl-2的表达。

In this article, the latest progress in the study of DNA condensation in vitro induced by multivalent cations is reviewed covering the mechanism, process, influencing factors of DNA condensation and its recent applications in gene transfection.

本文综述了近十几年来以多价阳离子为缩合剂诱发的DNA体外缩合的研究进展，着重介绍了缩合的机理、过程、影响缩合的因素以及应用于转基因的最新成果。

Myoblast fusion is a naturally occurring process which can be achieved in vitro and provides a convenient system to study the mechanisms involved in fusion processes in general and muscle formation in particular.

Calpain-calpastatin系统对成肌细胞的细胞膜融合起着重要作用，且融合率取决于细胞中calpains与calpastatin的比例。

There is a significant increase with age in the number of micronuclei and missegregation. Nondisjunction of chromosome 21 is much more frequent than loss in vivo and in vitro. No significant difference in nondisjunction between male and female was noted.

在不同年龄正常人外周血淋巴细胞中，微核数随年龄的增加而增加。21号染色体不分离与年龄的关系极显著，无论在体内还是体外，21号染色体不分离远远高于丢失。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上，於是我们将SPANXA1转染在CL1-5细胞株中，并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力，探讨SPANXA1对癌细胞性状的影响，结果发现增加SPANXA1会降低癌细胞的转移能力，在另一方面，我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中，并以西方点墨法再次证实SPANXA1是存在於核质中。

The group disposed by Acetylspiramycin doesn"t show parallel results; The strip gray scale of exotoxin A decreased gradually with the increasing dosages of Azithromycin and Roxithromycin, which doesn"t show in group disposed by Acetylspiramycin and group PAO-JP2; The strip gray scale of lasR, detected by RT-PCR, decreased gradually with the increasing dosages of Azithromycin, which doesnt show in group PAO-JP2.Conclusions: There were obvious differences between PAO1 and PAO-JP2 in biology characteristics in vitro.

本研究发现大环内酯类药物在生物被膜培养过程中分别处理PAO1、PAO-JP2，结果提示大环内酯类药物(14、15元环)通过对QS系统的影响可有效抑制铜绿假单胞菌生物被膜的形成；通过QS系统有效抑制铜绿假单胞菌的毒力因子的产生，16元环的大环内酯类药物乙酰螺旋霉未见上述结论；lasR基因的反转录结果提示阿奇霉素可在转录水平抑制QS系统发挥其作用。

Results In vitro ,daphnetin exhibited potent schizontocidal activity comparable to chloroquine at the dose range of 1～10 μmol/L. In vivo ,50 or 100 mg/kg.d -1 ×4 d daphnetin i.g. and 10,50 or 100 mg/kg.d -1 ×4 d dephnetin i.p. showed antimalarial efficacy comparable to CQ 10 mg/kg.d -1 ×4 d i.g. in mice infected with P. berghei ANKA, evaluated by both the reduction rate of parasitemia on D 4 and the average surviving days in 30 days.

结果 体外试验中瑞香素在 1～ 10 μmol L剂量范围内有明显杀灭裂殖体作用，而体内试验中按D4减虫率与感染鼠在 30d内的平均生存天数评价，5 0或 10 0mg kg 。d- 1 × 4d瑞香素灌胃以及 10 ，5 0或 10 0mg kg.d- 1 × 4d瑞香素腹腔注射给药在伯氏鼠疟原虫ANKA株感染鼠中的抗疟作用与 10mg kg 。d- 1 × 4d氯喹灌胃的疗效相似。

The infectivity of porcine endogenous retrovirus with porcine skin fibroblast cells in vitro and in vivo were demonstrated in this study, and there is no evidence of active viral replication of PERV in vivo.

本实验证实了猪皮肤成纤维细胞PERV的体外感染性和在小鼠体内的感染性，但未能找到PERV在体内活跃复制的明显证据。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。