查询词典 in vitro

Objective To investigate the effect of all-trans retinoic acid on the change in intracellular glycogen in ovarian epithelioma cell line in vitro and ovarian epithelial carcinoma in nude mice in vivo.

目的 探讨全反式维甲酸干预后卵巢癌细胞株及裸鼠卵巢上皮性癌动物模型肿瘤细胞内糖原的变化及意义。

In addition, the maximum velocity in larvae was significant higher than those in pupae and adults. In vitro inhibition studies of larvae AChE indicated that the I50 values of 20-d larvae were higher than those of 10-d larvae when treated with eserine, malaoxon and carbaryl, respectively, suggesting that AChE in 20-d larvae was less sensitive to the inhibitors.

通过对幼虫AChE离体抑制作用测定表明，毒扁豆碱、马拉氧磷和西维因对20d龄幼虫的抑制中浓度I50均高于10d龄幼虫，说明20d龄幼虫体内的AChE对3种抑制剂的抑制作用更不敏感。

Its losing weight unique efficiency is high, can in-depth seepage subcutaneous fat organization, activate the fat enzyme activity comprehensively, urges the fatty cell activity, the combustion unnecessary fat, the fast decomposition unnecessary fatty acid, through in vivo microcycle, the fast decomposition leaves in vitro abreast in row, the control energy takes, after preventing thinly, bounces the regeneration and the excrescence stores up, achieves models the body us body curve.

其瘦身独特效率高，能深层渗透皮下脂肪组织，全面激活脂肪酶活性，促使脂肪细胞活动，燃烧多余脂肪，快速分解多余脂肪酸，通过体内微循环，快速分解并排出体外，控制能量摄入，阻止瘦后反弹再生和赘肉囤积，达到塑身美体曲线。

In order to evaluate the effects of calcium ion on calpalns mRNA expression and proteolysis activity,and muscle protein degradation,L6 myoblast and beef fascicle were used in vivo and in vitro samples respectively in this study.

本实验分别以牛肉肌束和分化为肌细胞的L6成肌细胞为研究对象，研究钙离子对离体肌束中钙激活蛋白酶活性、蛋白分解以及活体肌肉中钙激活蛋白酶mRNA水平表达的影响。

Advance basic research has confirmed that the alkaloids extracted from thunberg fritillary bulb which is widely used in clinical medicine in vitro and in animal experiments have reversed MDR of leukemia. It will increase resistance of acute leukemia cells anticancer drug concentration and lower resistance the expression of Pgp. Pre-Clinical Experimental Research also shows that the conventional chemotherapy combined with the powder of thunberg fritillary bulb for the treatment of acute leukemia. Clinical complete response rate was higher, especially for patients with refractory or relapsed leukemia patients better clinical remission rate. In addition, Caladium particles Slices with chemotherapy in the treatment of RAL Clinical studies also show the Chinese refractory leukemia improve clinical efficacy safe and reliable.

先期基础研究已证实：临床常用的化痰散结中药浙贝母提取的生物碱体外及动物实验均有逆转白血病多药耐药的生物活性，能增加耐药的急性白血病细胞内抗癌药物浓度，降低耐药蛋白Pgp的表达；临床预实验研究也表明，常规化疗方案配合浙贝母粉用于急性白血病临床治疗，其临床完全缓解率明显高于对照组，尤其对难治及复发白血病患者临床缓解率更佳；此外，单药浙贝母颗粒配合化疗治疗RAL的临床研究亦显示中药在提高难治性白血病临床疗效方面安全可靠。

Prepare 131I-labbelled anti-CD20 monoclonal antibody using lodogen iodine labelling;(2)Measure the immunity activation of labbelled antibody by means of both cell combine analysis and LDH cytotoxicity detection kit;(3)Evaluate the injury rate of tumor exposed to 131I-chimeric anti-CD20 monoclonal antibody in vitro. The MTT method and the experiment of cell growth control are used;(4)Record the apotosis of Daudi cell using gelose electrophoresis;(5)Learn the inhibition effect of radioactive medication on the marrow. Radiohnmunoimages are taken on various intervals after injection of the labeled antibodies to the nude mice. The distribution of radioactive medication, I31l-labeled antibodies, in the marrow tissue indicates the inhibition effect. Here a B cell non-hodgkin lymphoma model in nude mice is established by us;(6)28-day observation are done in 3 groups of nude mice model.

(1) 用lodogen标记法制备131I-国产人鼠嵌合抗CD20单抗；(2)采用细胞结合分析法和乳酸脱氢酶法细胞杀伤性实验测定131I标记后国产人鼠嵌合抗CD20单抗与靶细胞的免疫活和利结合能力；(3) MTT法和细胞生长抑制实验测定131I-国产人鼠嵌合抗CD20单抗的体外杀瘤活性；(4)用琼脂糖凝胶电泳法研究131I-国产人鼠嵌合抗CD20单抗致Daudi细胞凋亡；(5)建立荷人B细胞淋巴瘤移植瘤裸鼠模型，应用γ计数法检测注射到荷瘤裸鼠体内的131I-国产人鼠嵌合抗CD20单抗的组织分布情况，明确其靶向性；(6)将荷瘤裸鼠分3组进行放射免疫治疗，分别为阴性对照组、131I-国产人鼠嵌合抗CD20单抗组、国产人鼠嵌合抗CD20单抗组。

In this paper,the effect of ginsenosides on hemolysis in human red cells in vitro and in dog red cells in vivo was investigated.

本实验研究了人参总皂甙在体外对人红细胞及在体内对狗红细胞的溶血作用。

Experiment in vitro showed that ONOO〓 led to an increase in MDA content in normal lung homogenate in a dose-dependent manner.

4体外实验显示，ONOO〓剂量依赖性的导致正常肺组织匀浆脂质过氧化产物MDA含量明显增多。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

RESULTS The proliferation of fibroblasts was inhibited by K102 in vitro. A dose-dependent antagonistic effect was found between K102 and bFGF. The serum levels of ALT, AST and the liver hydroxyproline level in the treated groups were lower than that in the CCl4 model group. Pathological examination showed a slight fatty degeneration and hydropic degeneration was observed in the group treated with the high dose K102, the low dose K102, and the control group.

结果体外生物活性检测：K102明显抑制成纤维细胞的生长，bFGF对K102有一定拮抗作用，且具有剂量依赖性；体内生物活性检测：治疗给药组血清ALT，AST和肝组织羟脯氨酸含量较CCl4模型组明显改善。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。