查询词典 in vitro

According to the mechanism of block of development in vitro culture on early embryo of mammal and in vivo surroundings of early embryo, the paper states that requirement and utilization of nutrients during each cell stage of early embryo of mammal in vitro culture in order to search for in vitro culture condition and method to improve the development rate of blastosphere.

摘要从哺乳动物早期胚胎体外培养发育阻断机理和早期胚胎的体内环境入手，阐述了胚胎体外培养各细胞阶段胚胎对营养物质的需求，寻求合理的体外培养条件和方法，以便提高体外胚胎早期的囊胚发育率。

The paper summarizes studies of in vitro inducement of haploids in cucurbitaceae, including anther culture, in vitro gynogenesis culture and induction of haploid obtained by irradiated pollen. The factors affectting in vitro inducement such as material genetype, medium, pretreatment, megaspores development stage and others had significant effects on the embryoids inductivities.

重点对葫芦科植物离体诱导单倍体的研究进行综述，包括花药培养、离体雌核培养、辐射花粉诱导单倍体培养等在育种中的应用，以及供体基因型、培养基、预处理方式、胚囊发育时期等因素对离体诱导再生频率的影响。

Results: Tyroserleutide can significantly increase the life span of H22 tumor-bearing mice by 50-70% in dosages of 20ug/kg/d-80ug/kg/d,specially the high dosage of 80ug/ml can significantly increase the life span by 69.24%; Tyroserleutide can inhibit the growth of transplanted hepatocellular tumor BEL-7402 in nude mice,the rate of tumor inhibition was25-50% in dosages of 40-320ug/ml ,the inhibition rate of 160ng/ml was 44.03%; Tyroserleutide could inhibit the growth of H22 and BEL-7402 tumor in a dose-dependent manner. Simultaneously, tumoricidal activity of tyroserleutide against BEL-7402 cell line in vitro was observed hinger when compared with the control group(P.05).The inhibition effect of 72hrs was higher than 24hrs,48hrs,96hrs.And specially the high dosage of 160ug/ml can significantly inhibit growth of tumor cell by 19.36%. Tyroserleutide can activated PEM and marked enhance cytotoxicity andphagocytosis functions in vitro and in vivo. The OD values of cytotoxicity were observed hinger when compared with the control group(P.05).The cytotoxicity of macrophages activated by tyroserleutide against BEL-7402 and B16-F10 was 35.58%,61.2% in vitro and21.39%,47.63% in vivo. The cytotoxicity rate of nude mice PEM was 32.86%,73.07% in vivo. Furthermore, tyroserleutide alone could stimulated the production of IL-1B TNF- a and NO by M . Tyroserleutide and LPS could synergistically activated M producing more cytotoxicity effectors. Conclusion: Tyroserleutide had inhibition functions against hepatoma carcinoma .Its possible mechanisms were related to the affect that Tyroserleutide could inhibit tumor cell directively and induce tumor cells apoptosis or death effectively.

结果：酪丝亮肽能显著延长腹水型肝癌H_(22)小鼠的生存时间，给药剂量为80μg／kg／d时疗效最显著，达到69.24％，在20μg／kg／d-80μg／kg／d剂量范围内生命延长率为50-70％，给药剂量与荷瘤鼠生存时间呈现一定量效关系；酪丝亮肽能显著抑制人肝癌BEL-7402移植瘤裸鼠的肿瘤生长，给药剂量为160μg／kg／d时疗效最显著，抑制率为44.03％，并且在40-320μg／kg／d剂量范围内抑制率为25-50％，给药剂量与肿瘤抑制率呈现一定量效关系；酪丝亮肽体外对人肝癌BEL-7402细胞生长有一定的抑制作用，在作用72hrs时各浓度酪丝亮肽对肿瘤细胞的抑制作用较24hrs、48hrs、96hrs明显，其中浓度为100μg／ml时抑制率达19.36％；酪丝亮肽体内外均能增强小鼠腹腔巨噬细胞对肿瘤细胞的杀伤：体外作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与效应细胞对照组相比有显著性差异(P＜0.05)杀伤率分别达到35.58％、61.2％；体内作用中巨噬细胞对BEL-7402、B16-F10的杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P 。05)，杀伤率分别达到21.39%、47.63%；裸鼠腹腔巨噬细胞经酪丝亮肤作用后对BEL一7402、B 16一F10杀伤功能明显增强，与生理盐水对照组相比有显著性差异(P.05)，最高杀伤率分别达到32.86%、73.07%；酪丝亮肤能增强单核巨噬细胞系统的吞噬功能，吞噬指数与生理盐水组比较有显著性差异(P.05)；酪丝亮肤体外作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与效应细胞对照组相比有显著性差异(P.05)；酪丝亮肤体内作用能促进小鼠腹腔巨噬细胞分泌合成细胞毒效应分子IL一lp、TNF一Q和NO，与生理盐水对照组相比有显著性差异(P.05)；酪丝亮肤能促进鼠巨噬细胞株R戌W264.7分泌合成IL一1p和NO，IL一1日、NO水平分别在酪丝亮肤作用24hrs、12hrs时达到高峰，酪丝亮肤单独应用能提高巨噬细胞的分泌合成功能，而且酪丝亮肤能与LPS协同作用刺激巨噬细胞的细胞毒效应分子分泌合成。

In this paper, 1554 strains of bacteria were isolated from the soybean rhizophere in the different continuous systems. Those biocontrol bacteria were screened in vitro and in vivo. 6 strains of them had the better control efficacy. They exhibited the different antagonism to the pathogens of soybean root in vitro. The strains 18BR2-2 and 18BR9-1 showed a wide range of antagonism against Fusarium oxysporum, F. avenaoeum, F. solani and Rhizoctonia solani in vitro. The 6 strains exhibited different control efficacy to soybean root rot in greenhouse and in field. In greenhouse, they could decrease the degree of soybean disease significantly, and the control efficacy of 18BR2-2 was the best and can reach 45.90%. In theory, soybean treated by 18BR2-2 may postpone the days of outbreaking of disease for 6 days. The strain 18BR2-2 also promoted the growth of soybean seedling and, raising the soybean height for 11.24%, increasing dry weight for 40.91% and fresh weight for 35.27%.

本研究从长期连作田种植的大豆及其根际土壤中分离细菌1554株，采用平板测试、温室盆栽及田间试验等方法，筛选了对大豆根腐病菌(主要是尖孢镰孢菌Fusarium oxysporum)有很好作用效果的生防细菌6株，在离体的条件下均表现出不同程度的拮抗作用，其中生防细菌18BR2-2和18BR9-1表现出很广的杀菌谱，对大豆根腐病菌尖孢镰孢菌(F.oxysporum)、燕麦镰孢菌(F.avenaoeum)、茄腐镰孢菌和立枯丝核菌都有很好的离体拮抗作用，并且这6株生防细菌在盆栽和田间的情况下均表现出对大豆根腐病一定的防治效果。

Part of program-controlled automatic traps (square design, automatic control) CBS-A 358 × 345 × 250 in vitro collection: 100, the maximum capacity of each: 12ml timing range: 1 second -9999 seconds, choose a sub--- 9999 points, arbitrary choice movement: a programmable control program number: 10 in vitro disk: Optional, 16/36/100/169, free combination (matching A disc-type tube can be resistant to organic solution)(L-type matching in vitro expansion of the collection plate may be) power-off function to preserve data and programs, retention time: Ten years characteristics: square design, XY movement, high-precision liquid crystal display a blue screen, the whole operation of the Chinese menu.

程控全自动部份收集器 CBS-A 358×345×250 收集试管：100支，每支最大容量：12ml 定时范围：1秒—9999秒，任意选择 1分—9999分，任意选择移动方式：可编程控制程序数：10个试管盘：可选配，16/36/100/169，自由组合（选配A型试管盘可耐有机溶液）（选配L型试管盘可扩容收集）断电数据和程序保存功能，保存时间：十年特点：方型设计，X-Y移动方式，高精度液晶蓝屏显示，全中文菜单操作。

Program-controlled part of the collection of fully automatic multi-function devices (square design, automatic control)(blue screen of liquid crystal display)(the whole operation of the Chinese menu) CBS-B 358 × 345 × 250 in vitro collection: 100, the maximum capacity of each: 12ml Timing range:-9999 seconds 1 seconds choose 1 -9999 arbitrary selection drops sub-range: 1 drop -9999 peak drops the scope of arbitrary selection choose :0-1000mv movement: programmable control program number: 10 in vitro disk: Optional, 16/36/100/169, free combination (SP-36L type matching in vitro expansion of the collection plate may be) power-off function to preserve data and programs, retention time: Ten years characteristics: CBS -B than the CBS-A is more applicable to high-end laboratory.

程控多功能全自动部份收集器方型设计，自动对管） CBS-B 358×345×250 收集试管：100支，每支最大容量：12ml 定时范围：1秒—9999秒任意选择 1分—9999分任意选择定滴范围：1滴—9999滴任意选择定峰范围：0-1000mv 任意选择移动方式：可编程控制(带废液槽程序数：10个试管盘：可选配，16/36/100/169，自由组合（选配SP-36L型试管盘可扩容收集）断电数据和程序保存功能，保存时间：十年特点：CBS-B比CBS-A更适用于高端实验室。

Computer program-controlled part of the collection of fully automatic multi-function devices (square design, automatic control)(blue screen of liquid crystal display)(the whole operation of the Chinese menu)(with software / computer operator) CBS-C 358 × 345 × 250 in vitro collection: 100 branch, the maximum capacity of each: 12ml Timing range:-9999 seconds 1 seconds choose 1 -9999 arbitrary selection drops sub-range: 1 drop -9999 peak drops the scope of arbitrary selection choose :0-1000mv movement: programmable control program number: 10 in vitro disk: Optional, 16/36/100/169, free combination (SP-36L type matching in vitro expansion of the collection plate may be) power-off function to preserve data and programs, Save time: Ten years of computer interface: RS232C serial port self-Computers: Computer software can be characterized by: CBS-C than the CBS-A / B is more applicable to high-end laboratory.

电脑程控多功能全自动部份收集器 CBS-C 358×345×250 收集试管：100支，每支最大容量：12ml 定时范围：1秒—9999秒任意选择 1分—9999分任意选择定滴范围：1滴—9999滴任意选择定峰范围：0-1000mv 任意选择移动方式：可编程控制程序数：10个试管盘：可选配，16/36/100/169，自由组合（选配SP-36L型试管盘可扩容收集）断电数据和程序保存功能，保存时间：十年电脑接口：RS232C串行口自配电脑：可实现电脑软件操作特点：CBS-C比CBS-A/B更适用于高端实验室。

And the vitro protein digestive ability of each digestive organ is in the sequence of stomach＞ foregut＞pylorus cecum＞midgut＞hindgut. The mean vitro digestive coefficient of feedstuff dry matter is in the sequence of Peru fish meal＞homemade fish meal＞ shrimp meal＞meat meal＞feed yeast＞soybean meal＞earthnut meal＞cottonseed meal＞wheat bran＞middling, and the mean vitro digestive coefficient of feedstuff protein is in the sequence of Peru fish meal＞shrimp meal＞homemade fish meal＞meat meal＞feed yeast＞soybean meal＞earthnut meal＞middling＞cottonseed meal＞wheat bran.

十种饲料原料的干物质体外消化率依次为进口秘鲁鱼粉＞国产鱼粉＞虾粉＞肉粉＞饲料酵母＞豆粕＞花生粕＞棉粕＞麦麸＞次粉，蛋白质体外消化率依次为进口秘鲁鱼粉＞虾粉＞国产鱼粉＞肉粉＞饲料酵母＞豆粕＞花生粕＞次粉＞棉粕＞麦麸，动物性饲料原料的干物质、蛋白质体外消化率明显高于植物性饲料的相应体外消化率。

The morphology of virions seems to not be change after incubated with the paeonol by electron microscope. The regained infectivity of TMV recovered by dialysis from incubation with paeonol in vitro showed that the paeonol have not direct viricidal effect. The paeonol did not reduce infectivity when applied before TMV inoculation. The paeonol in low concentration can restrain the polymerization of TMV coat protein in vitro. The paeonol did not reduced infectivity of TMV-RNA in vitro. It is proposed that the paeonol inhibited TMV by binding with TMV coat protein to interfere with recognition of a host receptor essential for infection or by interfering TMV-CP polymerization in plants. The anti-TMV activity of low molecular weight chemicals extracted from Polygonum cuspidatum were determined.

丹皮酚与TMV混合，电镜下观察对粒体形态没有影响；TMV与丹皮酚混和后透析可恢复侵染力，表明丹皮酚对病毒粒体并没有直接毒害作用；预先用丹皮酚喷施叶面不影响病毒的侵染，表明丹皮酚未干扰TMV对叶片侵染位点的识别；丹皮酚在低浓度时可阻碍TMV-CP的体外聚合，对TMV-RNA并无体外抑制侵染作用，据此推测丹皮酚对TMV的抑制作用可能主要通过其与TMV-CP结合影响病毒粒体对叶片表面侵染位点的识别，或影响体内TMV粒体的脱衣壳或TMV-CP亚基的体内聚合。

ABSTRACT Antitumor Activity of Dendritic Cells Activated with gP96 Peptide Complex Department of Thoracic Surgery, Peking University People's Hospital Objectives: To investigate the expression level of gP96 in human lung cancer samples; purify gP96-polypeptide from human and mouse lung adenocarcinoma cell lines; prepare tumor vaccine by in vitro gP96-polypeptide stimulation of monocyte-derived dendritic cells and test its efficiency both in vitro and in mice models.

研究人肺癌组织热休克蛋白gP96—多肽复合物的表达水平；肺癌细胞株来源的肿瘤组织gP96—多肽复合物的提取和鉴定；外周血来源树突状细胞培养和鉴定：将gP96—多肽复合物和DC体外共同孵育制备疫苗，在体外激活T淋巴细胞的能力及在小鼠体内的抑制肿瘤生长作用的研究。

Alternatively, the con- trollers can use the synchronous rectifier itself or loss- less inductor current-sensing methods to provide overload protection with lower power dissipation.

另外，康威特罗勒斯可以使用同步整流器本身或亏损减少电感电流检测方法，以提供低功耗过载保护。

Mr. Dauber's other Schatz hangs in his home movie studio.

多伯把沙兹的另一件作品挂在家里的电影室。