查询词典 in vitro

The aim of the research was to establish asymbiotic germination and low-temperature in vitro conservation technique system of Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro.

［目的］利用组培技术建立冬凤兰非共生萌发和低温离体保存技术，以达到快速繁殖和长期离体保存该植物的目的。

The ai mof the research was to establish asymbiotic germination and low-temperature in vitro conservation technique systemof Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-termconservation in vitro.

利用组培技术建立冬凤兰非共生萌发和低温离体保存技术，以达到快速繁殖和长期离体保存该植物的目的。

EGF has the auxoaction on proliferation of the cultured fibroblasts in vitro. Collagen scaffold has some regulatory effects on proliferation rate of the cultured fibroblasts in vitro.

EGF对FB的增殖具有促进作用，而胶原网架对FB的增殖速率具有一定的调节作用。

Results 30～80μg/ml 23-hydroxyl betulinic acid in vitro and 300mg/kg～600mg/kg P.O.in vitro markedly inhibited the growth of B16 cells at a dose-dependent manner with a great number of apoptotic cells seen.

结果 23-羟基桦木酸体外30～80μg/ml处理，体内300mg/kg～600mg/kg灌胃时，对B16细胞有显著的生长抑制作用，有一定的剂量依赖关系并出现大量的凋亡细胞，同时体内肿瘤细胞被阻滞在G0～G1期，DNA合成受阻，肿瘤体积缩小。

These transgenic cells were able to secrete hEGF protein to certain extent, and have the biological activities to enhance the growth rate of Hacat cells in vitro. Conclusion: Adult epidermal keratinocytes in vitro transfected with exogenetic hEGF cDNA can express and secrete active hEGF.

质粒pcDNA3.1-hEGF在脂质体介导下成功转染成人皮肤角质形成细胞，转基因细胞能分泌有生物活性的EGF；体外培养的成人皮肤角质形成细胞可见少量EGF分泌。

Methods The in vitro cultured porcine vascular endothelial cells and human sera were co-reacted to set up an in vitro model of hyperacute rejection of pig-to-human xenotransplantation.

用体外培养的猪血管内皮细胞和不同人的血清共同反应，建立猪与人异种移植超急性排斥反应的体外实验模型。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

Abstract］objectiveto observe antiseptic effect and bacteriostatic concentration in vitro of tieshanxuanyangping.methodsto determine bacteriostatic concentration in vitro for staphylococcus, bacillus coli, red trichophyton, purple trichophyton, trichophyton mentagrophytes, microsporum gypsen with doubling dilution.

摘要］目的观察&铁扇癣痒平&体外抗菌效果和抑菌浓度。方法采用对倍液体稀释法测定该药对金黄色葡萄球菌、大肠杆菌、红色毛癣菌、紫色毛癣菌、须毛癣菌和石膏样小孢子菌体外抑制作用。

That GER cells which are likely hair cell progenitors could be cultured in vitro and generate stereociliary bundle-like structure when forced to express Hath1 suggests that the misexpression of Hath1 probably can induce the predifferentiation of pure hair cell progenitors into hair cells in vitro.

GER细胞作为毛细胞前体细胞可以实现体外原代培养，在Hath1基因重表达情况下，部分细胞的表面可见不成熟的纤毛样结构，提示Hath1基因也许可以诱导离体培养下的纯的毛细胞前体细胞向毛细胞的初步分化。

Pharmacological activity test in vitro All target compounds and PFT-αwere assessed for their ability in vitro to protect PC12 cells against H2O2 induced apoptosis through MTT methods. The results suggested that compound 3a, 3b and 3c have dual activity of neurotrophy and apoptosis inhibiting activity on oxidation stress damaged PC12 cell.

三、抗神经细胞凋亡实验对目标化合物都进行了体外药理活性试验：以H2O2为凋亡诱导剂损伤PC12细胞构建氧化应激损伤的凋亡细胞模型，用MTT法考察目标化合物对H2O2诱导的神经细胞凋亡的抑制作用。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。