查询词典 in vitro

This dissertation studied the isolation, purification, culture system optimization and transplantation techniques of goat spermatogonium in vitro respectively to construct an optimized long term culture system to facilitate in vitro manipulate studies for future.

十年来小鼠精原干细胞培养和移植探索的不断完善，为各种哺乳动物及人类精原干细胞培养体系的建立和移植实验提供了新的思路和技术平台。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1）叶片对辐射敏感，叶片再生的半致死剂量为10 Gy-20 Gy，'丰香'品种比'章姬'品种敏感； 2）辐射后8 d-15 d接种，'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势，而其他剂量则几乎未见有变化，'章姬'在剂量10 Gy-30 Gy，叶片的不定芽再生率提高较大，40 Gy仍表现轻微下降； 3）适宜的辐射剂量对草莓试管苗增殖有促进作用。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上，利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中；对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

The transter and expression of endostatin gene was analyzed by PCR, the effects of inhibition on proliferation of endothelial cell by endostatin in vitro by MTT method, and the effects on the growth of LoVo、 SGC-7901 in vitro was surveyed, and the growth of expressing endostatin LoVo、SGC-7901 cell line_in subcutaneously of nude mice was also measured.

用MTT法检测内皮抑素对内皮细胞体外增殖的抑制作用，并观察其对LoVo细胞和SGC-7901细胞体外生长的影响，观察表达内皮抑素基因的LoVo和SGC-7901细胞在裸鼠皮下种植的生长。

The effect of chromium trichloride on miotic maturation and fertilization ability of mouse oocyte was studied by in vitro culture of mouse oocyte and in vitro fertilization.

采用小鼠卵母细胞体外培养，体外受精的方法研究了三氯化铬对小鼠卵母细胞成熟和受精能力的影响。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

The results indicate that:(1) Using SUZI method can improve the fertilization rates of denuded oocytessignificantly. This result suggests zona pellucida may be one of the reasons that makedenude oocytes matured in vitro have a clearly deficiency on fertilization in vitro.

结果表明：(1)利用精子带下注射技术可以极显著的提高去除卵丘细胞的卵母细胞的受精率，表明透明带结构的改变是去除卵丘细胞的卵母细胞存在受精障碍的重要原因之一。

Aim To establish an in vitro insulin resistant model of HepG2 cells and 3T3-L1 adipocyte and to screen drug in vitro.

目的 在体外建立肝胰岛素抵抗细胞模型和脂肪细胞胰岛素抵抗模型，并初步应用于中药有效成分的胰岛素抵抗活性筛选中。

Objective: To investigate the co-cultured allogenic SD rat bone mesenchemal stem cells in vitro, and to perform neural differentiation in vitro.

目的：探讨同种异体大鼠骨髓间充质干细胞在体外的共培养方法，并行神经分化诱导。

To establish a model of piglet testis tissue cultured in vitro,the testis's tissues of 30-day-old piglets were used to observe in vitro spermatogenesis,and the differentiation of androgone was identified pre- and post-culture by transmission electron microscopy and HE.

以30日龄仔猪睾丸组织块为培养对象，应用飞利普TECNAI10电子显微镜对培养前后的生精细胞类别进行了观察，对培养后的组织块进行生精细胞分化程度鉴定，建立了仔猪睾丸组织生精细胞爬片体外培养模型。

Fancy gold-plated dangling earrings with facetted White Opal crystals.

花式镀金悬垂耳环与facetted白欧泊水晶。

This essay chooses the study aim from biology teachers in middle school in Shi Jiazhuang which tells us that most of the middle school biology teachers in Shi Jiazhuang have the"burnout", lower successfulness, individualize.

本文选取石家庄市初中生物教师作为研究对象，运用问卷调查的方法对石家庄市初中生物教师职业倦怠的现状进行调查，调查结果发现，石家庄市初中生物教师这一群体普遍存在职业倦怠，情感枯竭程度偏高，成就感偏低，去个性化程度最为严重。