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A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

A chromium free conversion coating at least equivalent in corrosion protective quality to conventional chromate conversion coatings can be formed on metals, particularly cold rolled steel, by a dry-in-place aqueous acidic liquid comprising: a component of anions, each of said anions consisting of at least four fluorine atoms and at least one atom of an element selected from the group consisting of titanium, zirconium, hafnium, silicon, and boron, and, optionally, ionizable hydrogen atoms, and optionally, one or more oxygen atoms; a component of cations of elements selected from the group consisting of cobalt, magnesium, manganese, zinc, nickel, tin, zirconium, iron, and copper; the ratio of the total number of cations of this component to the total number of anions of component being at least 1:5; sufficient free acid to give the composition a pH in the range from 0.5 to 5.0; a component selected from the group consisting of phosphorus-containing inorganic oxyanions and phosphonate anions; and a component selected from the group consisting of water-soluble and water-dispersible organic polymers and polymer-forming resins and, preferably, also including a component selected from the group consisting of tungstate, molybdate, silicotungstate, and silicomolybdate anions.

通过对包含以下成分的含水酸性液体进行干燥，可以在金属上形成无铬的转换涂层，该涂层与普通铬酸盐转换涂层相比，至少在抗腐蚀质量上等效：$_一种阴离子成分，所述各个阴离子包括至少4个氟原子，至少选自钛、锆、铪、硅与硼元素中的1个原子。任选地可离解的氢原子，任选地1个或多个氧原子；$_一种选自钴、镁、锰、锌、镍、锡、锆、铁与铜元素的阳离子成分，上述成分的阳离子总数与成分内的阴离子总数之比至少为1∶5；$_足够的游离酸以使该组合物的pH值在0.5～5.0的范围内；$_一种选自含磷的无机含氧阴离子与膦酸盐阴离子的成分；$一种选自水溶性与水可分散性有机聚合物与聚合物形成的树脂的成分，优选还包括一种选自钨酸盐、钼酸盐、钨硅酸盐与钼硅酸盐阴离子的成分。

In the water samples from Gudao, and No. 1 and 2 wastewater stations of the Shengli Oil Field, the ratios of amount of iron bacterial cells to total microbial cells, detected by the combination of the 4 probes and DAPI staining, were 11.0%, 12.8% and 9.0%, respectively. In the iron bacterial community, Leptothrix, Sphaerotilus and L. ferriphilum were dominant in the 3 stations, and Leptothrix and Sphaerotilus, and L. ferriphilum cells to accounted for 2.94%±0.52%~3.39%±0.52% and 2.24%±0.16%~2.63%±0.49% of the total microbial cells, respectively, while L.

结果表明，利用基因探针能快速对胜利油田外排水中的铁细菌进行检测：在孤岛采油厂、胜利采油厂1号和2号综合处理站的外排水中，利用4种探针的组合探针检测到的铁细菌细胞数量分别占样品中微生物总细胞数量的11.0%、 12.8%和9.0%，其中Leptothrix和Sphaerotilus，以及Leptospirillum ferriphilum为胜利油田外排水中的3种优势铁细菌，分别占水样中总微生物细胞数的2.94%±0.52%~3.39%±0.52%和2.24%±0.16%~2.63%±0.49%；而Leptospirillum ferrooxidans和Acidithiobacillus spp。

Microbiotic crusts had a high capacity of evaporation than the shifting sand dunes. During the evaporation process of one week after rainfall, the total evaporation in artificial vegetation regions constructed in 1956, 1964, and 1981 were 6.05, 4.99 and 4.23 times respectively as the total evaporation in shifting sand dunes.

微生物结皮有着比流沙区更高的蒸发能力，在雨后一周的蒸发过程中，1956、1964和1981年人工植被区的总蒸发量分别是流沙区总蒸发量的6.05、4.99和4.23倍。

The both diets could also reduce the serum levels of triglyceride and free fatty acids, but the CLA diet caused an increase in the serum levels of total lipid and cholesterol. In contrast, the OFO diet caused a reduction in the serum levels of total lipid and the cholesterol.

血脂质方面，CLA饮食与炸油饮食均会使血清三酸甘油酯与游离脂肪酸降低，但CLA饮食会增加血清总脂质与胆固醇含量，相对餵食炸油饮食其血清总脂质与胆固醇仍然降低。

Results In patients treated tumors using 153 Sm-EDTMP alone, the total ostealgia remission rate was 61.67%, the total response rates of metastatic foci eliminating or shrinking was 20.00%; while in patients treated tumors using 153 Sm-EDTMP in combination with 99 Tc-MDP, they were 88.89% and 44.44%, respectively.

结果 单独使用153Sm-EDTMP治疗，缓解骨痛的总有效率为61.67%，转移灶消除或缩小的总有效率为20.00%，而153Sm-EDTMP与&云克&联合治疗则分别为88.89%和44.44%，两组间比较差异有高度显著性（P均。01）。

Results In patients treated tumors using 153 Sm-EDTMP alone, the total ostealgia remission rate was 61.67%, the total response rates of metastatic foci eliminating or shrinking was 20.00%; while in patients treated tumors using 153 Sm-EDTMP in combination with 99 Tc-MDP, they were 88.89% and 44.44%, respectively.

96例确诊为多发性骨转移肿瘤中，60例单独使用153Sm-EDTMP治疗，36例使用153Sm-EDTMP联合&云克&治疗，比较两种方法的治疗效果。

There was significant difference in the areas of alpha zone atrophy and total peripapillary atrophy among the three types of glaucoma. The areas of alpha zone atrophy and total peripapillary atrophy were bigger in POAG and NTG eyes than in CACG eyes.

不同类型青光眼之间：α带面积、浙江人学硕卜学位论文P队总面积均有组lbJ差异(分别为：介6.812，p=0.002；F=3.438，p=0.036)，其中POAG组与NTG组均比CACG组大(分别为：a带面积比较t=一3 。540，厂0.001；拼一3.170，尸一0.003；pPA总面积比较t=2.978，尸=0.005；户2.780，p一0.009)，PoAG组与NTG组。

In this before-and-after trial in a community private practice setting with extensive experience in pilonidal disease, 31 patients with severe refractory pilonidal disease had undergone a total of 141 operations, with wounds still open for a combined total of 252 years.

这个在民间社区所进行有关潜毛症的临床试验中，参与的31位全是顽固性潜毛症患者，所有患者总共做过141次手术，伤口保持开放的时间总共也有252年，其中27人平均追踪时间长达20个月。

A new data management algorithm for anycast based random sampling in sensor networks;2. Composite random sampling algorithm for scheduling concurrent projects;3. In the existing methods of random summing of total resource,the shape of the total resource curve sometimes distributes skewly,in view of which,the new method was put forward to improve random sampling .

针对现有的资源总量随机求和中存在的资源总量曲线形态有时呈偏态分布的问题，提出了对其随机抽样方法的改进方案，即把随机抽样入口由原来定义在(0，1)区间上改为定义在(0，ki)区间上(ki为第i个局部地质单元的石油资源量保险系数)，利用(0，ki)区间上均匀分布的随机数对风险分析后的资源总量分布函数进行随机抽样。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。