查询词典 in the wild

The Joker originated in the United States around 1857, used as a wild card in poker and as the highest trump in Euchre.

Joker这张牌的起源，是在1857年的美国，在扑克牌里面当作鬼牌，或是在尤克牌戏中做最大的王牌。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

John and Josie's only child [00:28.23]young and handsome, fast and wild [00:33.58]join in the army at 18 [00:38.74]left the army at 18 [00:44.46]drift then from job to job [00:49.81]town to town and love to love [00:55.17]7 years forever, till young Jonnie came marching home [01:05.85]but Jonnie wasn't looking well [01:10.63]went into the hospital [01:16.41]days turned into several weeks [01:21.73]Jonnie kept on losing weight [01:27.10]Josie said that John sit down [01:32.64]John cried oh god,not my son [01:37.77]Jonnie left at 25 [01:42.39]hole as big as Jupiter [01:49.25] [01:50.10]there's nothing stronger than a love [01:55.21]John and Josie head for Jonnie [02:00.60]throughing his last birthday party [02:06.10]no one from the family came [02:10.04] [02:11.27]no one from the family came [02:16.42]turns John and Josie's grift to shame [02:21.58]shame to anger,anger pain [02:27.05]nothing they needed more now [02:30.28] [02:53.50]2 years's gone, [02:55.38]the telephone is ringing in the dim the damn [03:04.07]beneath the world and cluser face [03:08.93]but John had punched the day he learned [03:14.49]that Jonnie he wouldn't live much longer [03:20.06]now a voice said to his father [03:24.74]Jonnie have a baby here [03:30.11]no one seems to want him [03:33.90] [03:35.94]baby doesn't have his parents [03:40.49]2 months old and with a pair of eyeless [03:46.65]Josie grabbed the phone from John [03:51.79]two of them said yes yes yes [03:57.51]so John and Josie over 60 [04:02.52]taking in a brand-new baby [04:07.78]named Paul Antonio [04:13.29]Jonnie would've loved him [04:18.24] [04:18.99]nothing stronger than a love [04:23.90]John and Josie head for Jonnie [04:29.32]the same love at they've fall apart [04:34.59]they starting up to win again [04:39.71]and on the fear that same could happen [04:45.27]losing paul before his time comes [04:50.53]John and Josie looked right past it [04:55.51]they're basketing in love's mighty presents [05:01.34]John and Josie don't have time [05:07.98]they're basket in love's mighty presents

约翰和Josie歌词翻译悬赏分：5 -离问题结束还有13天22小时 [00 ：23.00]约翰和Josie的独生子 [00 ：28.23]年轻和英俊，快速和狂放 [00 ：33.58]加入军队在18 [00 ：38.74]被留下军队在18 [00 ：44.46]漂泊然后从工作对工作 [00 ：49.81]镇对镇和爱到爱 [00 ：永远55.17] 7年，年轻Jonnie来了前进在家 [01 ：05.85]，而是Jonnie没有很好看 [01 ：10.63]进入医院 [01 ：16.41]天把变成几个星期 [01 ：21.73] Jonnie在丢失的重量保持了 [01 ：27.10] Josie说约翰坐下 [01 ：32.64]约翰哭泣oh神，没有我的儿子 [01 ：37.77] Jonnie离开在25 [01 ：42.39]孔一样大象木星 [01 ：49.25] [01 ：50.10]那里比爱是没什么强 [01 ：55.21]约翰和Josie头为Jonnie [02 ：throughing他的前次生日聚会的00.60] [02 ：06.10]没人从家庭来了 [02 ：10.04] [02 ：11.27]没人从家庭来了 [02 ：16.42]转动约翰和Josie的grift到羞辱 [02 ：21.58]羞辱愤怒，激怒痛苦 [02 ：27.05]他们现在需要的没什么 [02 ：30.28] [02 ：去的53.50] 2年的， [02 ：55.38]电话在昏暗敲响该死的 [03 ：04.07]在世界和cluser面孔之下 [03 ：08.93]，但约翰猛击了他学会的天 [03 ：14.49]那Jonnie他长期不会居住 [03 ：20.06]声音对他的父亲现在认为 [03 ：24.74] Jonnie这里有一个婴孩 [03 ：30.11]没人似乎想要他 [03 ：33.90] [03 ：35.94]婴孩没有他的父母 [03 ：40.49] 2个月老和以一个对无眼 [03 ：46.65] Josie劫掠了电话从约翰 [03 ：51.79]二他们是认为 [03 ：57.51]如此约翰和Josie 60 [04 ：采取在一个全新的婴孩的02.52] [04 ：07.78]命名的保罗Antonio [04 ：13.29] Jonnie将爱他 [04 ：18.24] [04 ：18.99]没什么强比爱 [04 ：23.90]约翰和Josie头为Jonnie [04 ：29.32]同一爱在他们有散开 [04 ：34.59]再开始由胜利决定的他们 [04 ：39.71]和在恐惧同样可能发生 [04 ：45.27]丢失的保罗在他的时间之前来 [04 ：50.53]约翰和Josie看起来正确的过去它 [04 ：55.51]他们在爱的强大礼物basketing [05 ：01.34]约翰和Josie没有时间 [05 ：07.98]他们是篮子在爱的强大礼物

In early spring, scattering along the endless stretches of black soil land dotted with traces of waning snow, a tough plant called "ice slush flower" sprouts out of ice slush and blooms its celebration of life. The mid-summer mountains are thickly blanketed with myriads of luxuriant trees. Across the marshes in late fall are unknown wild flowers and fruits competing in a delightful contrast, like a radiant symphony of colors of lemon yellow, rose, lilac, pink, light blue… In the coldest days of winter, the fields and mountains are a gleaming white immensity of snow.

初春，广袤的黑土地上残雪遍布，冰凌花顶开冰碴子顽强的张显着生存的力量；仲夏，万木扶疏，群山苍郁；深秋，沼泽里不知名的杂花野果相映成趣，柠黄、玫红、淡紫、粉红、浅蓝……汇成了一支缤纷的色彩交响曲；隆冬，山舞银蛇，寒妆素裹，白茫茫大地一片真干净——这就是孕育着张成艺术启蒙的原初营养。

In many ways, venture capital in China is like the wild west.

在许多方面，中国的风险投资就好比是狂野的西部。

But these were not the two Cats which I brought on Shore at first, for they were both of them dead, and had been interr'd near my Habitation by my own Hand; but one of them having multiply'd by I know not what Kind of Creature, these were two which I had preserv'd tame, whereas the rest run wild in the Woods, and became indeed troublesom to me at last; for they would often come into my House, and plunder me too, till at last I was obliged to shoot them, and did kill a great many; at length they left me with this Attendance, and in this plentiful Manner I lived; neither could I be said to want any thing but Society, and of that in some time after this, I was like to have too much.

这两只猫已不是我最初从破船上带下来的了，那两只早就死了，我亲自把它们葬在我的住所附近。不过其中一只不知同什么动物交配，生下了许多小猫。这两只就是我从那些小猫中留下来驯养起来的，其余的都跑到树林里成了野猫。那些野猫后来给我添了不少麻烦，因为它们经常跑到我家里来劫掠我的东西。最后我不得不开枪杀了它们一大批，终于把它们赶走了。所以，我现在有那么多仆人侍候我，生活也过得很富裕，唯一缺乏的就是没有人可以交往而已，其它什么都不缺。但不久之后，我就有人交往了，后来甚至感到交往的人太多了。

OUR scene is laid in Northern Jutland, in the so-called "wild moor."

我们现在在日德兰北部，在荒野沼地的另一边。

In the autumn of 1828, he was engaged to teach in the Indian School at Rice Lake; so called because large quantities of wild rice grew in this area twelve miles north of Cobourg, Ontario.

1828的秋季，他被指派去稻谷湖畔边上的一所印第安学校任教。之所以叫"稻谷湖"，是因为大量的野生稻谷长在安大略湖的Coboury以北12英里的这片区域。

Small-minded Edinburgh lawyers who disapproved of his campaigns might blackball him from their golf club, and did, but their slightly more sophisticated counterparts in London could hardly dismiss him as a wild leftie: they had known him in the Bullingdon Club and shared his views on hunting.

虽然与其政治立场相左的目光短浅的爱丁堡律师或许会在高尔夫俱乐部对他排挤，确实这样做了；然而，比他们更精于世故的伦敦同行们却无法将他当作左翼狂热分子视而不见：他们早在布灵顿俱乐部就已相识，并对打猎有着共同的志趣。

This example, formerly in the collection of Lieutenant-General Pitt-Rivers (1827-1900), is decorated with rows of wild animals carved in raised relief.

这个例子，在以前，收集中将皮特，河流（1827年至1900年）饰有雕刻救济行中提出的野生动物。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！