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Just prior to the analysis, prep a re the test solution by adding 0.5ml of the bromophenol blue indicator solution to each 100ml of the aqueous acetone solution and titrating with 0.01 N HCl or 0.01 N NaOH until the test solution is just yellow in color.

分析前准备好测试溶剂，100ml含2%水的丙酮加到锥形瓶中，加0.5ml 1%溴酚蓝水溶液指示剂，用0.01N NaOH溶液滴定至绿色或蓝色，然后用0.01N 盐酸标准溶液滴定至刚好呈黄色

The molecular sieve is prepared in the steps: at the room temperature, hexadecyl trimethyl ammonium bromide is mixed and dissolved with the sodium hydroxide, and is added with TEOS in dropping way, and simultaneously added with Me metal salt water solution and lanthanum nitrate solution in dropping way; the pH value of the mixed solution is adjusted by acetic acid to be 10 to 11, and is mixed for 1 to 2 hours after being stabilized; the sol is moved into a polyfluortetraethylene bottle to be heated and crystallized for 2 days in the oven at the temperature of 100 DEG C; after filtering, washing and drying for nights, and being calcined in a muffle at the temperature of 550 DEG C for 3 to 7 hours, the dual-metal-atom improved MCM-41 mesoporous molecular sieve is obtained.

该分子筛的制备为室温下，将十六烷基三甲基溴化铵和氢氧化钠混合溶解，搅拌并滴加入TEOS，同时滴加入Me金属盐水溶液和硝酸镧溶液并搅拌，用乙酸调节pH值至10～11，稳定后搅拌1～2小时，溶胶转入聚四氟乙烯瓶中在100℃下烘箱水热晶化2天，经过滤、洗涤、干燥过夜，马弗炉550℃焙烧3-7h，得双金属原子改性MCM-41介孔分子筛产品。

In the first part, first of all, we have a priori estimate for solution of the reaction-diffusion equation with initial condition and Dirichret boundary conditions. Then, we construct a semidiscrete Legendre pseudospectral scheme, and have a priori estimate for approximate solution. We discuss the asymptotic stability of approximate solution. At last, we get a error estimation on large time for solution of the semidiscrete system.

首先，对此类方程初边值问题的解进行了先验估计；然后建立方程半离散的Legendre拟谱格式，并对近似解进行了先验估计，讨论了近似解的渐近稳定性；最后，得到了半离散系统解的长时间误差估计。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

2Soil column leachate experiment shows, soil texture and soil section form are the main factors affecting the nitrate leaching; in soil organic fertilizer produce a nitrate leaching process, during five-month experiment, nitrate content has be increasing in leakage solution, organic fertilizer applying decrease the pH and increase the electric conduction of leakage solution, there is positive action between nitrate content in leakage solution and electric conduction, which shows that nitrate-N accumulation during organic fertilizer applying is one of the main factors for soil salification; for decrease the leaching of the nitrate-N in soil, in sandy moisture soil the best amount of organic fertilizer should be 7.5～ 15 t·hm~(-2), in Loamy moisture

在小麦-玉米轮作体系上投入化肥氮300kg·hm~(-2)N和有机肥22.3t·hm~(-2)折N261.2kg·hm~(-2，能使农田氮素收支达到平衡，在兼顾环境效益的基础上获得了较高的产量效益。并通过肥料效应函数和硝态氮累积效应函数进一步预测最佳施肥组合，即化肥氮施用量为N226.5kg·hm~(-2)，有机肥用量为39.9t·hm~(-2)折N466.8kg·hm~(-2，其中小麦季施化肥氮105.85kg·hm~(-2)N、有机肥30t·hm~(-2)折N351kg·hm~(-2，玉米季施化肥氮120kg·hm~(-2)N，有机肥9.9t·hm~(-2)折N115.8kg·hm~(-2。同时，磷素平衡分析表明，在施用有机肥的基础之上，化学磷肥的施用量应适量的减少或不施。

In order to enhance the miscibility between the inorganic and the organic phases,the silanol coupling agent is made from the slane VTMS and the colloid silica. Then the silanol coupling agent and nano-particles of ZnSb2O6 as the conductive filler are successfully bonded and the nano-particles of ZnSb2O6 will become more organic-like. The colloid silane-SiO2-ZnSb2O6 precursor solution is subsequently mixed with a homogeneous lsopropylalcohol solution of SR-399 acrylic monomers (Dipentaerythritol pentaacrylate) and the active free radical agent709. Under nitrogen purging, this reaction solution prepolymerize at 60℃. Then, the solution is spin-coated with the spin coator at room temperature. At last, the coated thin film is cured and crosslinked by the UV curing reactor to form the highly transparent thin film with good optoelectronic properties from hybrid organic-inorganic nano-composite.

首先以矽氧烷VTMS和colloid silica制备矽氧偶合剂，再以此偶合剂对分散於异丙醇溶液之奈米级的colloid ZnSb2O6做有机化改质，以增进其与有机高分子基材的键结相容性，我们将此colloid silane-SiO2 - ZnSb2O6前驱物与SR-399有机基材单体(Dipentaerythritol pentaacrylate)的异丙醇溶液与活性自由基光起始剂709均匀掺混后，在充满氮气反应系统下，加热至60℃进行预聚合反应，接著在室温下以旋转涂布机旋转涂布成厚度均匀薄膜，最后将此薄膜直接高温硬化或以UV硬化机网状键结硬化成透明与具部份导电性等光电特性之无机/有机奈米混成薄膜。

The effects of the buffer solution have been studied by many researchers and many experiments were also performed in this work. The results showed that NH〓Cl solution is the most suitable for the porous glass preparation and the mixed acid solution which used many times will lead to the high cracking of the glass and the new acid solution would be used every time.

很多学者研究了不同缓冲溶液对酸浸的影响，本文对此进行了大量实验表明，以NH〓Cl作为缓冲溶液浸析效果最好，表现为玻璃浸速率较快且易于获得完整的多孔玻璃，而重复使用含玻璃本身组分的酸液会造成玻璃开裂情况更加严重，因此建议使用新配酸液。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Iodine in the test solution was oxidized to IO-3 by excessive amounts of potassium permanganate solution and sodium hypochlorite solution,and excess of the oxidants was removed by reacting with sodium sulfite solution.

采用过量联合氧化剂高锰酸钾-次氯酸钠氧化试液中碘至IO3-，剩余的高锰酸钾和次氯酸钠用过量的亚硫酸钠反应除去，利用IO3-的电活性，建立了在四硼酸钠-吐温-80底液中的线性扫描伏安法测定微量碘的方法。

The construction of the solution space for shallow-water equations on the equatorialβ?plane and the equations about the tropical circulation to heating are mainly studied. The solution method of exact solution in the analytic function class and the computational procedure to the series solution are expressed for the well-posed problem.

平面近似的浅水方程组和热源强迫的热带环流方程组，重点研究了它们的解空间构造，并给出了各种适定的定解问题在解析函数类空间中准确解的具体的求解方法，并编制程序去计算其级数解。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。