查询词典 in situ

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

Expression of islet-1 in spinal cord of adult rat.In grey of the spinal cord of adult rat, the results of both of ISL1 immunohistochemistry and islet-1 mRNA in situ hybridi-zation showed that islet-1 gene expressed predominantly in the nuclei of motoneurons in ventral region and some cell around central canal and dorsal region. In white of spinal cord, the expression of islet-1 located in astroglia in anterior and dorsal funiculus.

在正常成年大鼠脊髓灰质中，ISL-l免疫组化和islet-lmRNA原位分子杂交结果都显示islet-1在腹侧角运动神经元中有相当强度的表达；在中央管周围和背侧角胶状质也有islet-1阳性表达细胞存在；在脊髓白质中，islet-l在腹侧索和背侧索中的星形胶质细胞有表达，外侧索相对较少。

The slices of pear leaf samples infected by apple chlorotic leaf spot virus were prepared for situ RT-PCR and were detected by primed in situ labeling technique using special primer.

选用带苹果褪绿叶斑病毒的香梨叶片，制备成适合进行原位RT-PCR的石蜡切片。

The ethnobotanical study of bamboo resources, is not only a prerequisite for sustainable development of bamboo resources, but also more importantly can be used for providing framework for feasible in situ and ex situ conservation by indigenous knowledge system.

许建初 ，裴盛基，陈三阳，王慷林竹类资源的民族植物学研究，不仅是竹类资源可持续发展的前期要求，而且最重要的是其能利用传统知识系统去建立一个适宜的就地和迁地保护的框架。

Objective To compare the postoperative visual quality after: laser in situ keratomileusis, laser-assisted subepithelial keratectomy and epipolis laser in situ keratomileusis.

目的 比较LASIK，LASEK与Epi-LASIK 3种不同的准分子激光手术术后患者视觉质量的差异。

The error between the retrieval and the in-situ data is descended to 53 percents, which is lower than the error between MERIS Level 2 and in-situ data.

通过对反演结果与实测数据进行分析，得出反演偏差可由二级产品90%相对误差降低到53%左右。

It will develop the in-situ X radiation sampling technique by exploration of in-situ X radiation sampling technique.

本文在X荧光现场技术方面的探索，将促进X荧光现场分析技术的进一步发展。

It applies the Fourierism-Gauss spectrum analysis on the in-situ X-ray instrument and advances the precision and the area of in-situ X radiation sampling technique.

本文的研究是对现场X荧光分析技术的一项补充，将原本在微机上使用解谱技术应用到现场X荧光分析中，提高X荧光的测量精度，扩大X荧光的应用范围。

Some suggestions on in-situ and ex-situ conservations are brought up in the article.

本文就海南坡鹿的就地保护和迁地保护提出了一些建议。

In-situ and ex-situ conservations are two conservation forms of Hainan eld's deer.

就地保护和迁地保护是海南坡鹿保护的两种基本形式。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。