查询词典 in situ

The development of research strategies and novel molecule methods in recent years have made a more careful analysis of HGT possible under natural and artificial conditions. Furthermore, horizontal transfer of catabolic gene in environment enhances biodegradation of recalcitrant organic compounds. Particularly, the delivery of catabolic gene information of conjugative plasmids to bacterial population in situ has great potential in bioenhancement.

近年发展起来的研究策略和新的分子生物学方法使人们能对自然和人工模拟状态下的水平基因转移进行更精细的分析，降解基因在环境中的水平传播促进了难降解有机物的生物降解，尤其是携带降解基因的接合质粒向原位细菌种群中的转移在生物强化中具有巨大的潜力。

The explanation for this is that ethylene oligomerized in the Ni catalyst active site and the oligomers containing vinyl end group served as comonomers to in situ copolymerize with the growing polyethylene chain in the active site of titanium catalyst.

催化剂的摩尔比、Al/摩尔比和聚合温度等聚合条件对催化活性及聚合物的结构与性能有明显影响。

Up to now, four types of cordaitean cuticles in situ and three types of dispersed ones have been reported from the Cathaysian Flora in China.

到目前为止，中国华夏植物区总共描述了 4种科达植物原位角质层和 3种分散角质层。

In the present study,in order to explore the roles of estrogen and progestin in epilepsy and elucidate their mechanisms of action, we utilized animal seizure models induced by intracerebroventricular coriaria lactone and intraperitoneal bemegride, studied the effects of estrogen and progestin on central nervous system functions in behavior,electrophysiological,cellular,molecular and gene levels by means of neuroelectrophysiology, flow cytometry, high performance liquid chromatography, immunocytochemical and in situ hybridization techniques.

为了探索雌、孕激素在癫痫发病中的作用，阐明其作用机制，本工作分别以马桑内酯侧脑室注射致痫、贝美格（Be腹腔注射致痫大鼠为实验对象，采用神经贫血电生理、流式细胞美白免疫荧光、高效液相色谱、美白免疫细胞化学、原位杂交早泻技术，从整体、行为、细胞、分子以及基因水平研究发现了雌、孕激素对大鼠中枢神经贫血系统功能的影响。

Methods: Integrated optical density was measured by ABC immuno-histochemical and in situ hybridization technique with computerized image processing. The rat′s brain was coronally sectioned after combination of EA and MEL.

采用ABC免疫组织化学和原位杂交组织化学技术，结合计算机图像处理，测定其积分光密度值。

Methods Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm×3 mm×4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with α1cDNA probe.

采取成年雄性SD大鼠肋软骨细胞，以3 mm×3 mm×4 mm大小的块状多孔HA为载体培养软骨细胞；培养3天和7天后通过扫描电镜观察HA表面和中矢横断面孔隙中有无细胞生长，同时将培养2天的细胞爬片以α1cDNA片段为探针进行原位杂交，求证培养的细胞是否软骨细胞。

RESULTS: Primarily cultured cells were colored with CD44 in-situ hybridization, kytoplasm as buffy and nucleus as blue by counterstain.

结果：原代细胞以CD44原位杂交显色，胞浆呈棕黄色，胞核经苏木精复染呈蓝色。

In order to investigate the role of ICAM-1 and VCAM-1 in the pathogenesis of glomerulonephritis, ICAM-1 and VCAM-1 expression were evaluated from both protein and gene level by in vivo and in vitro study, We have conducted (1) immunocytochemical analysis and in situ hybridization to examine the alteration in expression of ICAM-1 and it's relationship with interstitial infiltrating cells and TNFα; A total of 64 renal biopsies were classified in three groups according to the degree of cellular proliferation and infiltration in glomerulus;(2) indirect immunofluoresence and immunoblotting. methods to detect the VCAM-1 level both in renal tissue and in serum from the patients of lupus nephritis (17cases) and crescentic nephritis (4 cases);(3) Cell ELISA and northern blot technique to study the effects of TNFα and IL-1β on ICAM-1 and VCAM-1 surface expression and gene expression by cultured human mesangial cells .

为了探讨ICAM-1和VCAM-1在肾小球疾病中的作用，本文从蛋白质和基因两个水平，整体（研究ICAM-1时根据肾小球内细胞增生和浸润程度，将64例病人分为A.B.C三组）和细胞培养两个方面，做了如下工作，（1）利用免疫细胞化学和原位杂交技术观察了ICAM-1在64例肾小球疾病患者中的表达及其与间质浸润细胞、TNFα之间的关系；（2）利用间接免疫荧光和膜免疫印迹方法检测了VCAM-1在17例狼疮肾炎和4例新月体肾炎病人肾组织及血清中的表达；（3）利用细胞ELISA和Northern杂交技术研究了IL-1β或TNFα对体外培养的人肾小球系膜细胞ICAM-1和／或VCAM-1表面表达及mRNA表达的调节作用。

Fluorescence in situ hybridization and double FISH experiments were carried out to ascertain the chromosomal distribution patterns of the 45S and 5S ribosomal DNAs in the three species of Cucurbitaceae.

首次利用荧光原位杂交和双色荧光原位杂交技术对45S和5S rDNA在南瓜、丝瓜、冬瓜的有丝分裂中期染色体上进行了物理定位分析。

In situ Raman spectra of cycloheptane have been characterized under high pressure generated by diamond anvil cell at room temperature.

利用金刚石对顶砧装置产生高压，在室温下对环庚烷进行了高压原位拉曼光谱研究。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。