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To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Results1. Content of arsenic in serum raises with dose adding, time of pouring medicine and time of blood sampling prolonging. Thin layer chromatography indicates that there is toadfish in medicine serum of Liu-Shen-Wan.2. The suppressive rate of medicine serum of Liu-Shen-Wan on HL-60 cell raises with adding of dose and time of pouring medicine and prolonging of blood sampling.3. Gimsa dying assay, in situ end-labeling method and flow cell machine all exhibits: HI-60 cells dealed with Liu-Shen-Wan medicine-serum present typical apoptosis morphology.

结果：1 血清砷含量随着给药剂量、给药天数增加和采血时间的延长而升高；薄层色谱表明六神丸含药血清中有蟾酥成分。2 六神丸含药血清对白血病细胞的抑制率，随给药剂量、天数增加和采血时间的延长而升高3 吉姆萨染色、流式细胞仪检测、原位末端标记检测均证明六神丸含药血清能诱导HL-60白血病细胞凋亡。

Secondly,the phase separation mechanism,phase evolution and mechanical properties of toughener/BMI diphase system were investigated to prepare for toughening composite with Ex-situ technique.

其次，针对离位增韧中增韧剂与BMI树脂之间的相分离行为，研究了增韧剂与BMI树脂的反应诱导相分离机理、相形貌演化和力学性能。

Degradation is the most important factor during transformation from tumor in situ or pre-invasive tumor to invasive tumor.

浸润是转移的起始步骤，从原位癌或浸润前期发展到浸润性癌的转化中，最主要的因素是其下基底膜的破坏。

In situ AFM follows the transformation process of redox state of polypyrrole films and the change of the surface microstructure.

利用现场AFM技术跟踪测定了聚吡咯膜由氧化态到还原态的转变过程和表面微观形貌的变化。

Rapid prenatal diagnosis of trisomy 21 in 5049 consecutive uncultured amniotic fluid samples by fluorescence in situ hybridization

荧光原位杂交技术在产前遗传学诊断中的应用。

Then in situ hybridization of metaphase karyotype of male tufted deer are made use this two kind probes.

利用这两种探针分别对雄性毛冠鹿的中期染色体进行原位杂交，涂染探针的杂交得到了阳性结果。

A new instrument was designed to apply stress to the specimen of type 304 stainless steel. The effect of chloride concentration on the passive film crack potential was also studied in situ by using the electrochemical technology under constant strain.

采用自行设计的装置对304不锈钢试样施加拉应力，通过恒应变条件下的电化学原位测试研究了304不锈钢钝化膜破裂电位与氯离子浓度的关系。

Compared with the "three antibodies method", the sensitivity for detection of the signal of Y chromosome fluorescence in situ hybridization in brain tissue sections of mice was enhanced significantly by the direct tyramine signal amplification method.

结果 "三步抗体法"在脑切片检测Y染色体阳性信号的灵敏度和特异度分别为85.67％和100％；直接TSA法在脑切片检测Y染色体阳性信号的灵敏度和特异度分别为92.82％和100％。

Methods: Single lung in situ ischemic reperfusion animal model was used. 30 rabbits were randomly divided into three groups: sham group, ischemic/reperfusion group and ischemic preconditioning group. Wet to dry ratio and injured alveoli rate were determined. Ultramicroscopic structural change was observed under electron microscope. Heat shock protein-27 was detected with immunohistochemistry.

采用在体兔单位缺血瑞灌注模型，30只日本大耳兔随机均分为三组：假手术组、缺血／再灌注组及缺血预处理组，实验检测肺组织混干重比和肺泡损伤数比值，电镜评定肺组织起微结构损伤，免疫组化检测肺组织热休克蛋白27的定位及表达。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。