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In the present study,we intend to establish a new model of traumatic brain-steminjury,and to study the pathological changes of neuron,axon,astrocyte,plasmaproteins,blood vessel endothelia,synapse,neurotransmitter and cytokines in rat'sand human injured brain-stem,using routing histochemistry,immunohistochemistry,in situ hybridization,LSCM and computerized image analysis.

综合现有文献，本研究拟建立新的脑干损伤动物模型，并采用常规病理组化、免疫组化、原位杂交、LSCM以及计算机图像分析等技术，对动物及人体脑干组织中的神经元、星形胶质细胞、血浆蛋白及血管内皮细胞，以及轴索、突触、神经递质及细胞因子等进行研究，并对脑干损伤的致死性病变进行初步定位，以确立对脑干损伤的鉴定和推断脑干损伤时间有意义的客观指标。

The time to produce damage deformation in surrounding rock and possible loosening area are obtained by analyzing in situ measurement of displacement in soft weak coal layer.

本文通过对软弱煤层巷道进行位移实测及数据分析，得到了西部某矿软弱煤层巷道围岩变形达破坏所需的时间和可能的变形松动区域。

The terminology of"leaching solution"used in the domain of in situ leaching of uranium is discussed in the view of scientific rationality.

对我国地浸采铀中所使用的术语"溶浸液"的科学性提出了看法，供探讨。

The in-situ high-pressure structure of natural staurolite collected from the progressive metamorphic belt in Chonghuer of Altay area in Xinjiang was studied at room temperature using synchrotron radiation energy dispersive X-ray diffraction and diamond anvil cell under the pressure up to 3.9 GPa.

利用原位高压同步辐射能量色散X射线衍射技术，在室温下对采自新疆阿尔泰地区冲乎尔递增变质带的天然十字石进行高压晶体结构测定，测量的压力范围为0.0001~3.9GPa，共11组有效数据。

The RNA in situ hybridization experiments revealed that MOC1 was initially expressed in a few epidermal or subepidermal cells in the leaf axil prior to the formation of axillary meristem.

另外，我们发现在moc1突变体中由于MOC1基因的功能缺失突变，导致了分生组织特异性的OSH1基因和控制侧枝伸长的OSTB1基因的表达水平降低。

The RNA in situ hybridization experiments revealed that MOC1 was initially expressed in a few epidermal or subepidermal cells in the leaf axil prior to the formation of axillary meristem. The expression of MOC1 was continued during the formation process of axillary meristem. MOC1 was still expressed after axillary meristem has differentiated into tiller buds.

MOC1基因的组织原位杂交实验表明，在腋生分生组织形成之前，MOC1基因就已经在叶腋里的少数表皮或亚表皮细胞里表达，并且MOC1基因的表达伴随着腋生分生组织形态建成的整个过程；在腋生分生组织分化形成分蘖芽以后，MOC1仍然在整个分蘖芽中表达。

In this research , by using of genomic in situ hybridization , random amplified polymorphism DNA technology and three specific SCAR molecular markers, the author analyzed wheat hybrid generations carrying the desired genes that were resistant to take-all, BYDV and/or powdery mildew, and developed some alien chromosome addition and translocation lines that were important in breeding practice, and some specific molecular markers that could be applied in marker-assisted selection.

本研究运用GISH、RAPD和SCAR分析方法，对抗全蚀病、黄矮病和兼抗黄矮病白粉病的小麦杂交后代材料进行分析，获得了一些对育种实践有重要意义的易位系、异附加系材料和几个可用于辅助育种的特异性分子标记。

Methods The aim of this study is to examine the expression of VEGF-C and VEGFR-3 and CD31 molecule in rat embryonic lymphatic of the alimentary canal by immunohistochemistry and in situ hybridization in different periods.

采用免疫组化、原位杂交和原位PCR等方法对大鼠胚胎不同时期消化道组织的VEGF-C、VEGFR-3、CD31的表达情况进行观察、分析。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Results1. Content of arsenic in serum raises with dose adding, time of pouring medicine and time of blood sampling prolonging. Thin layer chromatography indicates that there is toadfish in medicine serum of Liu-Shen-Wan.2. The suppressive rate of medicine serum of Liu-Shen-Wan on HL-60 cell raises with adding of dose and time of pouring medicine and prolonging of blood sampling.3. Gimsa dying assay, in situ end-labeling method and flow cell machine all exhibits: HI-60 cells dealed with Liu-Shen-Wan medicine-serum present typical apoptosis morphology.

结果：1 血清砷含量随着给药剂量、给药天数增加和采血时间的延长而升高；薄层色谱表明六神丸含药血清中有蟾酥成分。2 六神丸含药血清对白血病细胞的抑制率，随给药剂量、天数增加和采血时间的延长而升高3 吉姆萨染色、流式细胞仪检测、原位末端标记检测均证明六神丸含药血清能诱导HL-60白血病细胞凋亡。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。