查询词典 in situ

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

In situ experimental study of culverts in the Qinghai-Tibet railway［J］.

青藏铁路涵洞试验工程研究［J］。

Results In situ hybridization of the three genes and immunohistochemistry showed that positive products were all located in the cytoplasms and nuclei.

结果：3种基因原位杂交、免疫组化阳性表达产物均分别定位于胞浆及胞核中。

Results In situ hybridization of the three genes and immunohistochemistry showed that positive products were all located in the cytoplasms and nuclei.

应用原位杂交法检测47例食管鳞状细胞癌及其癌旁、上切缘中3种基因的mRNA、免疫组化SABC法检测蛋白表达水平。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms purple-blue,NBP-BCIP.

结果：原位杂交检测显示，8例患者肺组织中都表达SARSCoVRNA，阳性信号位于靶细胞胞浆内，呈紫蓝色。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms （purple-blue, NBP-BCIP.

原位杂交和免疫组化结果显示：支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

Methods: the chondrocytes of the rabbit condylar were derived from rabbit,and were cultured to the second generation in vitro,then hgfcdna transfected the cells.the capacity of cell proliferation was tested by mtt,the deferences of the type ii collagen were tested by sabc immunohistochemical of the type ii collagen after infected;the mrna levels were tested nd so on the mrna levels were tested by in situ hybridization of the type ii collagen between the transfection group and comparison group .

取兔膝关节软骨细胞体外培养至第二代，hgfcdna转染软骨细胞，空白质粒组为对照组；噻唑蓝检测软骨细胞的增殖能力，sabc免疫组化及原位杂交法测其ii型胶原在转染前后的变化与mrna的表达。

They are OsMADS1, OsMADS5, OsMADS7 (OsMADS45), OsMADS8 (OsMADS24) and OsMADS34, in which OsMADS1 is currently the best characterized, the others are not documented well. On the basis of the results from dicots, we investigated the E function genes in rice using In Situ Hybridization and RNAi technique.

水稻是单子叶植物的模式植物，水稻中至少有5个E类基因，分别是OsMADS1、OsMADS5、OsMADS7、OsMADS8和OsMADS34 ，其中除了对OsMADS1基因有较深入的研究外，对其它几个E类基因的功能了解甚少。

Laser in situ keratomileusis for myopia from -7 to -18 diopters.

准分子激光屈光性角膜切削术与激光原位角膜磨镶术治疗中低度近视的对比。

Laser in situ keratomileusis for myopia from -7to-18 diopters.

角膜瓣蒂在上方的LASIK治疗近视。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。