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The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Phthalic acid esters are widely used in the many fields of industry. So many countries including China have come to pay attention to them and make up the codes for limiting the content in some products such as textile. By combining international codes, the thesis selects coating and textile that have close relative with people as objections. The research results are as follows:1. The thesis has established the textiles preparation method by a series of experimentations such as extraction solvent, extraction time, solid-phase extraction. The average recoveries of eleven phthalates are from 80.30% to 98.00%, and the relative standard deviations from 0.59% to 2.56%, the results show that this method is accurate and credible.2. The thesis set up the simultaneous determination of benzene, toluene, xylenes and phthalates in coatings.

邻苯二甲酸酯类化合物被广泛地应用在工业领域的各个方面，国际上已经开始重视邻苯二甲酸酯作为一种内分泌干扰素的危害性，对在一些产品中的用量作出了限制，但没有具体的分析测定方法，本论文结合国际法规选择了与人们生活密切相关的涂料和纺织品来研究其中邻苯二甲酸酯类环境激素的测定方法，研究内容如下：(1)通过对提取溶剂、提取方式、提取时间的选择实验以及固相萃取的一系列条件实验，首次建立了纺织品中测定邻苯二甲酸酯类环境激素的前处理方法，并对分析方法作了准确度、精密度验证。

The preparation of the main map are as follows:"Atlas of Hainan Province","National Defense Transportation Atlas of Hainan Province","Atlas of the education system in Hainan Province","Atlas of Hainan Province","practical map of Hainan Province","Hainan Province Map List,"" Earthquake defense plans,"" navigation map "," ICBC distribution network "," Distribution of the Marine "," Wanning City Map "and other kinds of more than 60 plans to update the 18 cities and counties in Hainan Province wallcharts complete Hainan Province, the first "electronic atlas" for the development and construction of Hainan, has played an important role in protection.

编制的主要地图有：《海南省地图集》、《海南省国防交通图集》、《海南省教育系统图集》、《海南省地图册》、《海南省实用地图》、、《海南省地图册》、《地震防御图》、《航标图》、《工行网点分布图》、《海事分布图》、《万宁市地图》等60多个图种，更新海南省18个市县挂图，完成海南省首份《电子地图集》，为开发建设海南起到了重要保障作用。

300Cr Spagyrics Vol 1 (195 pages), Spagyrics Vol 2 (206 pages)- Spagyrics: A Practical Course in Plant Alchemy by Jean Dubuis is a 2-volume, 48-lesson course which teaches the preparation and uses of herbal elixirs, particularly the seven planetary elixirs used to rebalance the spiritual centers in the human body, the processes followed to obtain the vegetable stone, the method for extracting the universal seed from rain water, and similar processes.

300cr spagyrics1卷(195页)， spagyrics2卷(206页)-spagyrics：实用课程植物炼金术1730杜博伟是一个2卷，48节课讲授的制备与应用中草药酏，尤其七个行星酏用来拉平的精神中心，在人体中过程之后取得的蔬菜石头提取方法的普及，从种子，雨水，而类似的过程。

200Cr Spagyrics Vol 1 (195 pages), Spagyrics Vol 2 (206 pages)- Spagyrics: A Practical Course in Plant Alchemy by Jean Dubuis is a 2-volume, 48-lesson course which teaches the preparation and uses of herbal elixirs, particularly the seven planetary elixirs used to rebalance the spiritual centers in the human body, the processes followed to obtain the vegetable stone, the method for extracting the universal seed from rain water, and similar processes.

200cr spagyrics1卷(195页)， spagyrics卷2(206页)spagyrics：实用课程的Jean-Pierredubuis厂炼金术是第二卷，48-课教课程准备和使用草药酏，尤其七个行星酏用来平衡人体的精神中心，随后进程取得蔬菜石材普遍的提取方法，从种子雨水，类似进程。

Experimental study on the metal surface cleaning agent re-oil preparation process of the series,Obtained by experiment the optimum cleaning process conditions:2g respectively SDBS,2g sodium silicate,2g sodium nitrite dissolved in 100 ml of distilled water,making the steel sheet coated with oil immersed in cleaning agents,under the conditions which keeping the washing temperature 30℃,Stirring rate of 2 files,Cleaning time for 5 min of the cleaning Cleaning the oil to achieve the highest rate of 99.62%,Corrosion rate can be controlled below 0.006%.

实验研究了金属表面重油污清洗剂制备的系列工艺，通过实验得出其最佳的清洗工艺条件：分别取2g十二烷基苯磺酸钠、 2g硅酸钠、2g亚硝酸钠溶于100毫升蒸馏水中，将涂有油污的钢片浸入清洗剂中，在清洗温度为30℃，搅拌速率为2档，清洗时间为5 min的条件下清洗，其油污清洗率最高能达到99.62%，腐蚀率能控制在？以下。

Our research included four parts: design and synthesis of amine oximes and other compounds as ligands, label them with 〓Tc〓, assessment of their biological activities in vitro and in vivo, and preparation of 〓 kit for clinic use.

研究工作主要包括合成作为配体的〓及其类似物，制备其〓Tc〓标记物，进行乏氧细胞实验和荷瘤动物实验，考察它们在肿瘤细胞中的吸收与体系乏氧的关系，以及在不同荷瘤动物模型中的放射性分布和显像情况。

EXCLUSIONS 4 In no case shall this insurance cover 4.1 loss damage or expense attributable to wilful misconduct of the Assured 4.2 ordinary leakage, ordinary loss in weight or volume, or ordinary wear and tear of the subject-matter insured 4.3 loss damage or expense caused by insufficiency or unsuitability of packing or preparation of the subject-matter insured (for the purpose of this clause 4.3 "packing" shall be deemed to include stowage in a container or liftvan but only when such stowage is carried out prior to attachment of this insurance or by the Assured or their servants) 4.4 loss damage or expense caused by inherent vice or nature of the subject-matter insured 4.5 loss damage or expense proximately caused by delay, even though the delay be caused by a risk insured against (except expenses payable under Clause 2 above) 4.6 loss damage or expense arising from insolvency or financial default of the owners managers charterers or operators of the vessel 4.7 deliberate damage to or deliberate destruction of the subject-matter insured or any part thereof by the wrongful act of any person or persons 4.8 loss damage or expense arising from the use of any weapon of war employing atomic or nuclear fission and/or fusion or other like reaction or radioactive force or matter.

EXCLUSIONS 4在任何情况下都不得这个保险 4.1损失损害或费用的原因故意的不当行为的保证 4.2普通渗漏，普通的损失重量或体积，或普通磨损的标的物投保 4.3损坏或丢失所造成的费用不足或不适合的包装或准备受保标的物（目的是这一条款4.3 &包装&应视为包括存放在容器或liftvan但只有当这些堆放进行之前扣押保险或担保或其雇员） 4.4损坏或丢失所造成的牺牲固有的副总裁或性质的标的物投保 4.5损坏或损失近牺牲所造成的延误，即使是延迟所造成的风险保险（除费用缴付根据第2段） 4.6损失损害或费用所产生的破产或财务预设的业主管理人员承租人或经营者的船只 4.7故意损坏或蓄意破坏受保标的物或其任何部分的不法行为，任何人 4.8损失损害或费用的使用中出现的任何战争武器的使用原子或核裂变和/或融合或其他类似反应或放射性武力或事项。

Solid super acid catalyst composed of iron oxide as active component has obvious advantages in its simple preparation procedure, good catalytic activity, especialilly much cheaper in comparison with catalysts prepared by other metal oxides such as titanic oxide and zirconia, it\'s worthy of industrialized investigation for these reasons.

以铁氧化物为主要活性组分制备的固体超强酸催化剂具有制备工艺简单、催化活性较好，尤其是相对于钛、锆等其它金属氧化物所制备的固体超强酸催化剂具有价格低廉的突出优点，值得进一步进行工业化的研究。

If you have been trained as a Registered Nurse in another country, and would like to work as a Nurse in Canada, the RN Exam Preparation Program is the right course for you.

如果您已培训作为一名注册护士在另一个国家，并希望作为一个护士在加拿大，护士考试预备方案是正确的当然你。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。