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Shen tonics and nourishing Yin were selected, and decided into the groups of liquid of complex prescription by the Yin-Yang principles of the treatment: IQ and NY group, YT and NY group, ST and NY group and so on, which were respectively boiled, administrated the rats via gavage and separated the sera of drugs for preparation, the sera were served in HCSMMC-7721 in vitro, normal sera of rats and Retin acid as matched controls, MTT colorimetric method was applied to assay cell proliferation.

选取临床肝癌治疗常用的益气、温阳、补肾、养阴等扶正治法的代表药物，并根据阴阳治则理论将其分别配伍为益气养阴、温阳养阴及补肾养阴等治法的小复方，分别煎成汤液，采取大鼠灌胃给药后分离药物血清的方法制备药物血清，作用于体外培养的SMMC-7721人肝癌细胞，以正常大鼠血清和维甲酸作对照。

In this paper, firstly using natural fatty acids including lauric acid, myristic acid, palmitic acid etc. and disproportionated rosin as starting materials, the acyl reaction of starting materials with SOCl_2 respectively, and then using Schotten-Baumann condensation: on the reaction conditions of alkalescence, condensation of acylchloride with amino acid (sarcosine, glycine, alanine etc.), and then acidification, saltation. A series of anionic surfactants of N-acyl amino: sodium N-fatty acyl sarcosinate (SFS-12, SFS-14, SFS-16); sodiun N-fatty acyl glycinate(SFG-12, SFG-14, SFG-16); sodium N-fatty acyl alaninate(SFA-12, SFA-14, SFA-16)and sodium N-disproportionated rosinoyl aminatewere prepared. During the preparation of N-acylamino acid, the reaction conditions of acylchloride with amino acid condensation were identified by optimizing the synthetic conditions of N-lauroyl sarcosine: mol ratio of amino acid to acylchloride 2:1, reaction taken place in a solvent composed by acetone/water 2:1, acylchloride and 20% NaOH were slowly added to the reaction mixture at the same time while maintaining the pH at 9~10, after completion of adding maintaining reacting for 2.5h at 25℃.

首先以月桂酸、肉豆蔻酸、棕榈酸等天然脂肪酸和歧化松香为原料，与氯化亚砜反应制得酰氯，然后采用Schotten-Baumann 缩合法路线，即在碱性条件下，酰氯和氨基酸(肌氨酸、甘氨酸、丙氨酸等)缩合，经过酸化、成盐，合成一系列氨基酸型阴离子表面活性剂：脂肪酰肌氨酸钠，即月桂酰肌氨酸钠(SFS-12)、肉豆蔻酰肌氨酸钠(SFS-14)、棕榈酰肌氨酸钠(SFS-16)；脂肪酰甘氨酸钠，即月桂酰甘氨酸钠(SFG-12)、肉豆蔻酰甘氨酸钠(SFG-14)、棕榈酰甘氨酸钠(SFG-16)；脂肪酰丙氨酸钠，即月桂酰丙氨酸钠(SFA-12)、肉豆蔻酰丙氨酸钠(SFA-14)、棕榈酰丙氨酸钠(SFA-16)；N-歧化松香酰基氨基酸钠(sodium N-disproportionated rosinoyl aminate)，即N-歧化松香酰基肌氨酸钠(sodium N-disproportionated rosinoyl sarcosinate ，简称SDRS)、N-歧化松香酰基甘氨酸钠(sodium N-disproportionated rosinoyl glycinate，简称SDRG)、N-歧化松香酰基丙氨酸钠(sodium N-disproportionated rosinoyl alaninate，简称SDRA)。

Due to the key challenge for complicated techniques and preparation, there were few reports about ternary systems. A series of polyimide/SiO_2-TiO_2 hybrid films with different contents of inorganic particle were fabricated using tetraethoxyl silicane and tetrabutyl titanate as precursor in situ with sol-gel method and both phases in situ. Chemical structure and micro-morphology of hybrid films were studied by means of IR, SEM and XRD.

本文以正硅酸乙酯和钛酸四丁酯为前驱体，采用原位溶胶-凝胶法和两相原位同步法制备了一系列不同含量的聚酰亚胺/SiO_2-TiO_2杂化薄膜，利用红外光谱、扫描电镜和XRD观察了薄膜的化学结构和微观结构，并测试了薄膜的热稳定性、电击穿场强和耐电晕性。

At the same time,we have tried the precess for the preparation of 3-exomethylene cepham sulfoxide ester by the improved ring-expanding method over Kukolja, in which the different acid scavengers are employed in the presence of tolune, NCP and stannic chloride.

同时尝试了改进的Kukolja扩环法合成3—环外亚甲基头孢亚砜酯。以甲苯为溶剂，NCP为卤化试剂，无水SnCl_4为路易斯酸，通过选用了不同的酸清除剂，以制备闭环产物。

In chapter 4, we have described an improved method for the preparation of ethynylferrocene. Ethynylferrocene was prepared in satisfactory yield by reaction of formylferrocene with triphenylphosphine and carbon tetrachloride followed by dehydrohalogenation in the presence of n-BuLi.

第三章中，我们研究了钯催化下碘杂环化合物的羰基化—酰胺化反应、羰基化—酯化反应和与末端炔烃的羰基化交叉偶联反应，为一步合成多官能团化合物开辟了新的途径，同时，得到的这些产物经过进一步官能团转化后，可以成为一些天然产物合成中的重要中间体化合物。

The product has been sold on the market for many years in foreign countries, but in China, only Zhejiang Conba Pharmaceutical Co.,Ltd (Jinhua, Zhejiang Province, China) has obtained national medicinal approval documentation currently, which may due to the unmatured preparation process of the product.

该产品在国外已上市使用多年，国内目前只有浙江金华康恩贝生物制药有限公司取得药品生产批准文号。

Methods: The interference evaluation experiment proves that IVIG exerts strong inhibitions in Bacterial Endotorxin Test. While the interfering factors can't be eliminated by treatments such as dilution and neutralisation, they are liable to be inhibited provided that the IVIG samples are to be diluted by 1:4 with the Dilution Solution Ⅰ.The BET for IVIG preparation diluted by 1:4 with the Dilution Solution Ⅰ is carried out comply with the Chinese Pharmacopoeia(2000 Edition) The lysate with sensitivity of 0.125Eu/ml is used in the test,and the results of the BET are comparable to that of Pyrogen Test carried out in rabbits.

通过干扰评价实验证明IVIG对细菌内毒素检查法有强烈的抑制作用，单纯用简单稀释和调整pH值法无法消除干扰，如果用稀释剂对IVIG作1：4以上的稀释，即可消除样品对该检查法的干扰，利用灵敏度为0.125EU/ml的鲎试剂，将样品用稀释剂稀释4倍，按中国药典（2000版）进行细菌内毒素的检查，结果与家兔热原质试验进行比较。

In fact, they do battle in the preparation, and actively make preparations for war with China, China is the new Asian powers, Japan is Asia's hegemony, the two countries-not fight.

其实他们在做打仗的准备，积极地做好跟中国战争的准备，中国是亚洲新列强，日本是亚洲霸权，这两个国家非打一仗不可。

The "water pool" in reverse micelle is formed by surfactant spontaneously arraying in non-aqueous solvent. This provides an excellent micro-reactor for the preparation of nanoparticles.

表面活性剂会在非水溶剂中自发排列形成纳米尺寸的&水池&，这为纳米粒子的制备提供了优良的微反应空间。

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。