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In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

为了获取这一基因片段与国外分离株进行同源性比较,同时也为了了解MDPV强、弱毒株VP2基因之间的异同关系,及其免疫原性的变化规律,通过DNA重组技术,设计了一对引物LHMP7/LHMP8,该对引物选取位于2885~2900及4618~4604的两段序列,跨幅为1734bp,并在这两条引物中分别加入两种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点,分别对MDPV-Q和由该株病毒经人工致弱后得到的MDPV-26株进行PCR扩增,并将PCR产物克隆到pMD18-T载体上,分别得到二个重组子:pMD18-T—M-Q VP2和pMD18-T-M-26 VP2。

Some bank staff mistakenly believed that even if these developers to do "fake mortgage" is also the capital flow in order to solve the problem, the risk is relatively low, for so-called increase in the volume of business, but also in order to "take care" of major clients, as a "non-malicious than benign fake mortgage" and for the purpose.

一些银行工作人员错误地认为,即使这些开发商做"假按揭",也是资本的流动,以解决这一问题,风险相对较低,为所谓的增加的业务量,而且还以"照顾"的主要客户,作为一个"非恶意比良性假按揭"和目的。

The main findings are that the reason why China chose to show force was in order to enhance China's own international status, the reason why the U.S. failed to deter China was because of its overcommitment in cooperating with China and low credibility in defending Taiwan, and the reason why China deliberately brought about the crisis was in order to increase its diplomatic influence.

主要的发现如下:第一、中国为了追求其在国际社会中地位之提升,而选择展示武力;第二、美国对中国之所以吓阻失败的原因是因为过度的合作承诺和低度的防卫承诺;第三、中国作为一个崛起的大国故意制造台海危机,以确保其外交影响力的增加。

In the act of apologizing, in order to reach optimization of the apologizing information, we need to make and choose the tactics according to such factors as purpose, environment, target of apologizing, etc. Then it turn materially concrete forms, and these different language forms embody the vocabulary, grammar, rhetoric' s characteristic.This text attempts the research of the act in order to passes the perspectivity of apology from the multi-disciplinary and many angles as the breakthrough point.

在道歉活动中,为了达到道歉信息传递的最优化、实现最优化的表达效果,需要依据道歉的目的、环境、对象等因素制定、选择策略,然后物化为具体的语言形式进行表达,这些不同的语言形式体现出语音上的、词汇上的、语法上的、修辞上的特点。

Therefore, in order to adapt to changes in the market demand, Chinas development of supermarket chains should be innovative supermarket business philosophy integration supermarket activities, Construction supermarket development platform cautious expansion and scale, while deepening the supermarket management in order to promote the development of chain stores.

因此,为适应市场变化的要求,我国的连锁超市在发展中应创新超市经营理念,整合超市业态,构建超市发展平台,谨慎扩张,追求规模效益,同时深化超市经营管理,以此来促进连锁超市的发展。

The stem was cut into several segments away from the pine seeding, arranged and collected in the order of chronology time and distance from where they were inoculated. To separate B.xylophilus coarsely out from the stems and made them into paraffin slices. And be observed under the potics microscope by dyed with safranin-fast green contrast-stain method and PI fluorescence stain method. In order to find out how about the nematode rigour distributing , but also ebb and flow of their amounts after invading the host pine seeding. Seek the pathological changing orderliness of host cells in different position along with the remotion of nematodes. By deeply analysing the variety of host cell nucleolus to estimate whether the host cell took place the programmed cell death after inoculated B.xylophilus.

按时间进程和与接种点上下距离的远近,对松苗分茎段进行粗分离和制作成石蜡切片,分别用番红-固绿双重染色法和碘化丙锭荧光染色法在光学显微镜下观察,以了解线虫侵染后在寄主体内的准确分布和数量的消长,寻找随着线虫的移动扩散,寄主不同部位细胞病变的数量、速度发生的变化规律,并进一步通过分析其细胞核的变化来判定松材线虫侵染后是否能诱发寄主细胞发生程序性死亡(programmed cell death,PCD)。

In real flight, multiple sensors of aircraft engine control system may fault at a time. In order to solve this problem, a revised multiple-failure-hypothesis based testing is investigated. This approach uses multiple Kalman filters, and each of Kalman filter is designed based on a specific hypothesis for detecting specific sensors fault, and then uses WSSR (Weighted Sum of Squared Residual) to deal with Kalman filter residuals, and residual signals are compared with threshold in order to make fault detection decisions. The simulation results show that the proposed method can be used to detect multiple sensors soft failures fast and accurately.

一般认为两个以上传感器同时发生故障的概率较小,但实际上不能排除其发生的可能性;提出了一种改进的多重故障假设方法来解决航空发动机控制系统多传感器同时发生软故障的问题,这一方法采用一组卡尔曼滤波器,每个滤波器对应一个被检测传感器的故障模态;采用残差加权二乘方处理滤波残差并与门限值作比较来判断是否发生故障;仿真结果表明,所提出的方法能有效解决多传感器软故障的检测与隔离问题。

In order to enable medicine return to its "merciful" nature, restitute the respect for patients, the dignity of doctors, find the memorable warmth in the medical service world, hospitals must pay close attention to humanistic care in the management dimension in order to establish a harmonious relationship between doctors and patients.

为了让医学回归"医本仁术"的本性,还患者以尊重,给医生以尊严,找回医患世界久违的温情,医院必须从管理层面重视人文关怀,并用以指导构建和谐的医患关系。

The sake of world peace, in order to eliminate all of the world war, proposed the establishment of "United Nations Presidential Palace","world government", the United Nations General Assembly changed to "United Nations Congress," issue "in the world yuan","World Unity Identity Card","World Unity law "," United Nations World University "," United Nations World Economic Group "," World's first Talent Festival "the idea of the peaceful reunification of the whole world, in order to achieve this great idea, running north and south river twenty true story.

为了世界和平,为了消灭全球所有的战争,提出建立"联合国总统府","世界政府",把联合国大会改为"联合国国会",发行"世界元","世界统一身份证","世界统一法律","联合国世界大学","联合国世界经济集团","世界首届人才节"和平统一全世界的设想,为了实现这伟大的理想,奔走大江南北二十年真实的故事。

First, this thesis introduces basic theory of CBR , and studies many aspects of typical part design case , such as case representation, case retrieval, retain etc. Second, regards design code as case code in order to integrate the case with geometrical and non- geometrical information, and realizes the parametric modifying function for 3D solid model in the condition of Pro/E. Third, for the purpose of making true of system's aided design requirement, this thesis brings forward to create part design knowledge base with AI language CLIPS, and integrates the rule based KB into the system; finally, collects and summarizes the knowledge of shaft,gear,sleave, and a sample with ladder—shaft is shown in order to verify the correction of theory research.

首先,本文介绍CBR技术的基础理论,并重点研究典型零件案例的表示、检索、修改等功能;其次,以零件设计编号为案例编号实现对案例的几何与非几何信息的集成,并实现了三维实体模型在Pro/E环境下的参数化修改功能;再次,从系统辅助设计功能要求出发,提出将基于规则的知识库嵌入到系统内部,用人工智能语言CLIPS建立零件设计知识库;最后,对轴类、齿轮类、盘套类零件案例知识进行收集、总结,并通过阶梯轴设计实例验证了理论研究的正确性。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?