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Results as bellows: AtSIRT1 was located in Mitochondrial as hSIRT4 of human, and maybe take part in respiration and electron transformation chain, AtSIRT2 was located in nucleolus as hSIRT6 of human, maybe play important role in extend lifespan;mutation in AtSIRT1 leaded to cotyledon of plant turn to yellow and caused short life span. Mutation in AtSIRT2 could make the color of leaf turn to purple and accumulate a lot of anthocyanin;Sirtinol, a inhibitor of SIRT which did not cause the same model of the mutation of AtSIRT1 and AtSIRT2 indicated that the mechanism of Sirtinol was different from other organism;the structure of AtSIRT1 and AtSIRT2 were similar to other known Sir2, which indicated that they maybe have the same function;AtSIRT2 was overexpressed and its activity was detected.

结果表明，1，拟南芥AtSIRT1与人的同源蛋白hSIRT4相同，定位于线粒体，可能参与呼吸作用和电子传递，SIRT2与人的同源蛋白hSIRT6相同，定位于细胞核，可能同它的功能类似，在延缓衰老及调节细胞寿命方面起作用。2，AtSIRT1突变，可引起幼苗和植株的子叶变黄和早衰；AtSIRT2突变，可引起叶片发紫，沉积大量花青素。3，SIRT蛋白的抑制剂Sirtinol不能表型模写AtSIRT1和AtSIRT2突变体，说明Sirtinol在拟南芥中的作用机制不同于其他生物。4，AtSIRT1和AtSIRT2蛋白质结构预测表明与已知的Sir2蛋白相似，揭示其功能的相似性。5，在大肠杆菌中过量表达了其中一个基因(AtSIRT2)，可体外检测其酶学活性，进一步证明其功能。

The phytoplasmas diseases were investigated in 30 counties and cities of 10 areas in Yunnan Province. There are 12 phytoplasma diseases, including paulownia witches-broom,chinaberry witches-broom, cactus witches-broom, christmas cactus witches-broom, orange little leaf, petunia flat stem and so on were indentified using PCR with universal primers, which amplified phytoplasma 16SrRNA gene and/or ribosomal protein genes. So the kind of phytoplasma diseases in Yunnnan Province were understood. Identified the characteristic of 16SrRNA and rp gene of phytoplasma and the kind, classification and genitic relativity of these strains using RFLP, clonging and sequence analysis (nearly 20 full-length sequences of phytoplasma 16SrRNA and/or rp gene were submitted in GenBank ); The phytoplasma strains isolated from herbage plants were conserved in storeroom. 9 articles were published in the key journals of China and 1 technique invention patent was applied. The net-page about phytoplasma disease was established. 4 graduate student were bringed up.

对云南省10个地区的30余个县市地区进行了植原体病害的调查采集工作；采用植原体16SrRNA基因通用引物（R16mF2/R16mR1及R16F2/R16R2）和/或植原体核糖体蛋白基因引物（rpF1/R1）对所采集到的病害标样总DNA进行PCR扩增，根据植原体特异扩增条带的出现，共鉴定出泡桐丛枝、苦楝丛枝、仙人掌丛枝、蟹爪兰丛枝、柑桔小叶、矮牵牛扁茎等12种植原体病害，初步明确了云南省植原体病害的种类；通过RFLP技术、克隆及测序技术获得其16SrRNA及部分核糖体蛋白基因的近全长序列（已在GenBank中共登录近20余个相关序列），通过序列比较分析，明确了上述植原体株系中这两个基因的特征并确定了云南省植原体株系的种类、分类地位及遗传相关性；对一些发生在草本植物上的植原体进行了株系保存；在国内核心期刊及一般期刊发表研究论文 9 篇，申请技术发明专利1项；初步建立了有关植原体病害的网页；培养研究生4名。

Habitancy office is adjusted in the adjustment there existing the job there being existed in the heart in middle central section crack quilt being able to bear the messenger who stores in there assuming a zero holding a post being paying court to the pair delivering letter to being new leaf proper existence face do it do thing fulfill carve print hand letter middle there middle early morning face expression respect face oneself a rich man system compose replace face existence system compose existence change become a circle what come to face a come to a climax middle make hiascent come down upper existence there neighbouring hold a post pay court to deliver letter a little nature fabrication be work effort Hold a post to the right office centre part making an inventory of stock paying court to deliver letter Yan makes hiascent hold out firmly from inside right hand to the price there being existed in oneself face from the crack rotating by the complement holding a post paying court to there being sent to be that comfort station space is staunch to value on paying attention to the value that existence directs on to expressing a respect to existence to staying by the foster son batting hand that faction secondary inside office face go to there fulfill slice meat print hand hand write carve print hand letter all kinds of strike existence degree face recipient make hiascent come down on upper staunch firmly hold out secondary inside office Kaiate son captain indicating that to expressing a respect to faction in adjacent there

Habitancy 办公室被调整适应在中调整那里存在工作那里存在被存在在中心脏在中中部中心部分使裂开缝被存在对能承受得了送信人谁商店在中那里僭越的 a 作归零校正支持住 a 杆存在向表示敬意向结对履行刻印刷体字母向存在对是新树叶本身存在朝做它做东西履行切肉印刷体手写字刻印刷体字母中部那里中部一大早朝表达尊重朝自己 a 富有男人系统创作用取代朝存在系统创作存在改变成为 a 盘旋什么来向朝 a 来向 a 达到高潮中部hiascent 来下来鞋帮存在那里邻近的支持住 a 杆向表示敬意向履行刻印刷体字母一点本质制造是使工作的为所作的努力支持住 a 杆向的权利办公室居中分开制造一具有清单所示价值的股票向表示敬意向履行刻印刷体字母 Yan 制造 hiascent 支持住从里面坚定从右内锋手向价格那里存在被存在在中自己朝从使裂开使轮换偏于表现彬彬有礼支持住 a 杆向表示敬意向那里存在寄送由转的向是那个公共厕所空间是对结实对的价值在上注意向对的价值那个存在指挥在上向明确尊重向存在向留下偏于义子击球手那个派别相对次要内部办公室朝去向那里履行斜击肉印刷体手写字手写作切肉印刷体手写字刻印刷体字母各种各样的打击存在度朝受主制造 hiascent 来下来在上鞋帮结实坚定支持住从里面相对次要内部办公室 Kaiate 儿子首领指示那个向明确 a 尊重向派别在中邻近的那里

All the contents of Extractable heavy metal like Pb, Cu, Zn and Cd in limestone slagged wasteland are higher than those in contrapositive soil nearby. The slag filtrate has no effect on seed germination rate of Mung beans, Broad beans and Corns but restrains their germination vigor and protraction of hypocotyls with root restraint rate exceeding 30-50%; While it has an apparent effect on germination rate and germination vigor of sensitive seeds like Beans with P=0.05. Simulate to plant the 4 different seeds in pot for a month, Test groups have a lower data in total leaf area, plant height and weight but a much higher content of protective materials like PRO and MDA in biochemical mensuration experiment compared with CK group.

石灰炉渣废弃地中Pb、Cu、Zn、Cd重金属有效态含量均高于临近对照土壤，其炉渣淋出液对绿豆、蚕豆、玉米3种植物种子发芽率无影响，但均抑制其发芽势和下胚轴伸长，根长抑制率达30%~50%以上，对敏感种子黄豆的发芽率、发芽势（4d）影响明显，摸拟盆栽一个月苗期，4种植物试验组在总叶面积、株高、生物量方面均低于对照组；生理生化测定，4种植物其体内抗逆性保护物质脯氨酸和膜过氧化产物丙二醛含量试验组均高于对照组。

The clear water that day of the 2-3 that irrigate air wants when watering is advisable, and when watering, should irrigate in plant ' cup ' in shape, in irrigating the matrix in flowerpot directly, even if slant in the matrix inside the basin dry, xie Beizhong has the word of water, plant still grows good, but if be the humidity in basin earth only big, and Xie Beizhong is anhydrous a plant is lacklustre, occurrence leaf easy hair is yellow wait for harmful response.

浇水时要浇晾2-3天的清水为宜，且浇水时要浇在植株的'杯'状中，不要直接浇在花盆的基质中，即使是盆内基质中偏干，叶杯中有水的话，植株依然生长良好，但假如只是盆土中湿度大，而叶杯中无水则植株无生气，出现叶子易发黄等不良反应。

You are the poem, let me have the inspiration which expressed You are the song, sing my long Tibet's in heart note You are the picture, let me have the dream which spread wipes You are the clock, knock my tight lock heart door You are the wind raise ,ripples you are the rain moisten my withered heart door leaf you are the fire let me have the burning desire you are the flower split decide the opening you are the mountain solid arm let me in mine heart snuggle up to you are the prairie vast chest hug me, you are big shake the Tao sound are your palpitation rush ,you are the dubhe tow I to lead the way the direction ,you are the rainbow let see the seven colors of the spectrum ray, you were the sun I bathe in the light are demented, you are the moon warmly caress my delightful dreamland I to rush to you: Herds in your vast chest snuggles up to listen to your pulse in your solid arm to shake, you are my illusion reposes, you are my mind relay station you are my soul companion ,you are in my heart spouse.

你是诗、让我有了抒发的灵感。你是歌、唱出我久藏在心中的音符。你是画、让我有了涂抹的梦想。你是钟、敲开我紧锁心门。你是风掀起我心海的涟漪你是雨滋润我干枯的心扉你是火让我有了燃烧的欲望你是花绽定开放在我的心里你是山结实的臂膀让我偎依你是草原辽阔的胸膛把我拥抱你是大海震震涛声是你心跳的澎湃你是北斗牵引我前行方向你是彩虹让看到了七色光芒你是太阳我在光中沐浴癫狂你是月亮暖抚我甜美的梦乡我奔向你：在你辽阔的胸膛放牧偎依在你结实的臂膀听你脉搏的震荡你是我梦幻的寄托你是我心灵的驿站你是我灵魂的伴侣你是我心中的爱人

Company products are mainly in the fields as follows: all kinds of fresh-cut flowers harvesting machinery, hierarchical classification of fresh-cut flowers, flowers produced water treatment line, bouquets bundlers / lines, flowers in addition to leaf machine, flower bulbs cleaning grading line (lilies, tulips, gladiolus, daffodils, iris, Freesia, hyacinth and other early spring flowering the kinds of the ball), refrigerated display equipment bouquets, flowers, container / flower buckets filling equipment, potatoes, onions automatic grading equipment, cucumber automatic classification line, product quality and technology in a leading position in similar enterprises in the Netherlands, sold 5 continents.

公司产品主要涉及的领域为：各类鲜切花采收机械，鲜切花分级分类，花束生产流水处理线，花束捆扎机/线，花枝除叶机，花卉种球清洗分级线(百合，郁金香，剑兰，水仙花，鸢尾，小苍兰，风信子和其他早春开花的种球)，花束冷藏展示设备，花卉容器/花卉水桶灌装设备，土豆，洋葱自动分级设备，黄瓜自动分级线，产品质量和技术处于荷兰同类企业领先地位，远销5大洲。

Under different stresses, we tested the expression of citrate synthase gene in rapeseed leaf by using semi-quantitative PCR. The expression of citrate synthase gene had no obvious change in stresses of salt, dark, high illumination, while was increased at different time in treatments of water logging, drought, IAA, and 6-BA. Interestingly the effect of ABA was contrary to that of IAA. In the treatment of sclerotium blight, the expression of citrate synthase gene was depressed. There was a saddle curve of citrate synthase gene expression in the treatment of gibberellin.

对油菜幼苗进行植物生长调节物质、高温和低温、强光照和弱光照、盐、菌核病、干旱和水渍等处理，采用半定量PCR法对油菜叶片柠檬酸合酶基因的表达模式进行检测，发现在盐胁迫、暗光和强光的处理下，柠檬酸合酶基因的表达基本没有变化；在水渍、干旱、IAA和6-BA胁迫下，其表达有所升高，但出现峰值的时间不同，ABA对表达模式的影响与IAA相反，感染菌核病后其表达降低；对GA3的应答呈鞍型。

The scenery of lake district is different along with the change of four seasons, in spring snow mountain melt Juan Juan snow-broth remit in lake, the cuckoo adornment surrounding country of mountain overflows , here is a slice of paradise of the wild animal who regains consciousness; In summer, between pale yellowish green forest, 100 flowers are opened greatly , have vast sea of clouds; In fall the lake water of dark blue fall to reflect the bank leaf with golden side, it is dry,crisp air of autumn; In winter cold congeal the earth, here, it is a slice of and peaceful.

湖区的景色随着四季的变化而不同，春天，雪山融化的涓涓雪水汇入湖中，漫山的杜鹃点缀四野，这里是一片苏醒的野生动物的乐园；夏天，葱绿的林间百花盛开，云海茫茫；秋天，碧蓝的湖水倒映着岸边金黄的树叶，秋高气爽；冬天，寒凝大地，这里一片宁静。

So the depth(20-40cm)of fertilizer application could be as best depthfor delaying the senescence of flag leaf and root system and gainning highyield and high efficiency in dry land wheat.5 The effects of deep cultivation-root cutting on the senescence after an-thesis and yield had been studied in dry land wheat of high yield.the best ef-fect obtained from treatments by deep cultivation-root cutting in beforewinter stage,next by the treatment in the starter stage treatment and in con-trol,lastly by the treatment in the before winter stage and starer stage.

由此认为延迟旱地小麦根系与旗叶衰老并获得高产的施肥深度应在20-40cm左右。5深耘断根对旱地高产小麦花后衰老及产量的影响进行了研究，以冬前深耘断根最好，起身期深耘断根与对照次之，冬前和起身期都深耘断根效果最差。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。