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By the present of DMF, attractions arising from hydroxyl groups increased, and it is more favored to forming hydrogen bonds between amino acids; repusings arising from apolar alkyl groups side-chaines have little change ,but they increase with increasing alkyl groups, llydroxyl groups assist in the formation of hydrogen bonds in interior protein molecules, alkyl groups assist in the highly extension of peptide chaines, these make protein structure stabilized.

而L-缬氨酸非极性侧基的排斥作用在15％乙醇中最大，随乙醇含量的进一步增加，排斥作用的影响逐渐减小，吸引作用的影响逐渐占据主导，且越来越大。

HP positive groups was compared with HP negative groups in gastric mucosal lesions, expression of P16 in HP positive groups was lower than that in HP negative (P＜0.01), expression of CD44V6, PCNA and C-met in HP positive groups were all higher than those in negative groups (P＜0.01). Expression of P16 was negative correlated with expression of CD44V6, PCNA and C-met in HP-infected gastric mucosal lesions (r〓=-0.6971, P＜0.01; r〓=-0.4811, P＜0.01; r〓=-0.6075, P＜0.01); Expression of CD44V6 were positive correlated with expression of C-met and PCNA (r〓=0.6550, P＜0.01; r〓=0.4977, P＜0.01). Selenium level in HP-infected gastric mucosal was Significantly higher than normal and the level will fall with deteriorism of gastric mucosal lesions.

临床标本研究结果表明：在系列胃粘膜病变中，P16表达HP阳性标本显著低于HP阴性标本（P＜0.01）；CD44V6、C-met、PCNA表达HP阳性标本均显著高于阴性标本（P＜0.01）；在HP感染的胃粘膜病变组织中，P16表达与CD44V6、C-met、PCNA表达均呈负相关（r〓=-0.697l，P＜0.01；r〓=-0.4811，P＜0.01；r〓=-0.6075，P＜0.01）；CD44V6表达与C-met、PCNA表达相互呈正相关（r〓=0.6550，P＜0.01；r〓=0.4977，P＜0.01）；HP感染的胃粘膜病变组织硒含量明显升高，并随胃粘膜病变程度加重相对降低，但高于正常组织；硒含量分别与CD44V6、C-met、PCNA表达阳性率呈负相关（P＜0.05），而与P16表达阳性率呈正相关（P＜0.05）。

The hydroxyl and carboxyl, belonging to auxochromic groups, were oxidized into the carbonyl and ester groups, belonging to chromophoric groups, during inducing discoloration under the pretreatment conditions, which makes the wood color deeper. There were plenty of auxochromic groups and chromophoric groups in the acetone extractives of silver chain. The interaction between the auxochromic groups and the chromophoric groups made the wood color deeper.

结论］羟基、羧基等助色基团在预处理条件下氧化成羰基、酯基等发色基团，使木材的颜色加深；刺槐木材丙酮抽出物的主要成分中含有大量发色基团和助色基团，这些基团间的相互作用使木材颜色加深。

3NE、MTT、LDH、ET-1、ICAM-1 and Tie-2experiment show: in the process of oxygen glucose deprivation/reoxygenation ,BMEC have subjected great damagement, I/R groups have significant differences compared with NC groups (p.05);polymorphonuclear leukocyte can aggregrate this injury, I/R+PMN groups have significant differences compared with I/R groups (p.05); after Sivelestat Sodium treatment, the effect of I/R injury has been decreased, I/R+PMN+Siv groups have significant differences compared with I/R+PMN groups (p.05), and NE、MTT、LDH experiment show that the three dose groups there was a significant dose-effect relationship.

3测定NE、MTT、LDH、ET-1、ICAM-1、Tie-2 mRNA均显示：氧糖剥夺/复氧复糖过程能对BMEC造成损伤，I/R组与NC组比较有明显的统计学差异(p.05)；中性粒细胞能加重对BMEC的损伤，I/R组与I/R+PMN组之间比较亦有统计学差异(p.05)；西维来司钠处理后，血管内皮细胞的损伤明显减轻，I/R+PMN组与I/R+PMN+SIR组比较有统计学差异(p.05)，并且NE测定以及MTT、LDH实验提示在西维来司钠三个剂量组之间存在明显的量-效关系，其对BMEC的保护作用有剂量依赖性。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

objective:to investigate the influence of soybean isoflavones on the antioxidative effect and improving the ability of study and memory in climacteric rats.methods:forty-eight female sd climacteric rats were randomly divided into 4 groups with one control group and the other low,medium and high dose groups,which were given soybean isoflavones 0 mg/kg.bw,42 mg/kg.bw,83 mg/kg.bw and 249 mg/kg.bw by superalimentation daily for 60 days respectively.the levels of sod,gsh-px and mda in serum were measured,and the ability of study and memory in each group was deterimined by adopting the law of diving platform.results:the content of serum mda in medium and high dose groups was lower than that in control group.the activity of serum gsh-px im medium and high dose groups was significantly increased,so was the activity of serum sod in low,medium and high dose groups.conclusion:soybean isoflavones do have the obvious effect of antioxidation,and improve the ability of study and memory effectively in female sd climacteric rats.

目的：观察大豆异黄酮对更年期雌性sd大鼠抗氧化作用及学习记忆的影响。方法：选用健康更年期雌性sd大鼠，随机分成空白对照组和低、中、高剂量4组。空白对照组给予蒸馏水，实验组每日分别灌服42、83、249 mg/kg剂量的大豆异黄酮，连续60d。第60d取血测定血清过氧化脂质丙二醛含量、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力，用跳台实验方法测定大鼠学习记忆能力。结果：与空白对照组比较，中、高剂量组大鼠血清mda含量显著降低，低、中、高剂量组血清sod活力明显升高，中、高剂量组血清gsh-px活力明显升高。结论：大豆异黄酮具有明显的抗氧化作用，并有效提高更年期雌性sd大鼠的学习记忆能力。

It is found that (1) the aquatic protozoa communities consists of thirty-six taxa in twenty-six genera and twenty-four families;(2) the groups of ecological function include: Photosynthetic autotrophs (P groups, 25%), Bactivores detritivores (B groups, 44.44%), Saprotrophs (S groups, 2.78%), Algivores (A groups, 11.11%), Nonselective omnivores (N groups, 11.11%) and Raptors (R groups, 5.56%);(3) according to the characteristics of karst water, substrates and mosses, four ecological types and eight sub-types of aquatic protozoa communities can be distinguished.

2水生原生动物群落含6个功能营养群。其中，原生动物光合营养群（P群，Photosynthetic autotrophs），占总数的25%；细菌取食者群（B群，Bactivores detritivores），占总数的44.44%；腐生营养类群（S群，Saprotrophs，占总数的2.78%；食藻者类群（A群，Algivores），占总数的11.11%；非选择性杂食者群（N群，Nonselective omnivores），占总数的11.11%；食肉者群，占总数的5.56%。(3)根据岩溶水流、基质和藓类植物特征，划分该区附生水生原生动物生态群落为4个类型和8个亚类型。

Results of the experimental groups: Comparison with the control groups, in 6h and 8h groups eminences of ependymal cells with cobblestone-like appearance were seen, their microvilli were further richer, and a number of microholes could be found on their base. In body part of SFO cell membrane of some ependymal cells were broken completely, their cytoplasm was no longer seen, and cell nucleus located at one side of the cell, 5-7μm in diameter and dividing lobe-like in shape. Beside those cells some ependymal cells appeared many folds on the top of surface. In 2h and 16h groups the observation results seemed no significant differences from the corresponding control groups.

实验组结果：与对照组相比，在6小时和8小时实验组中，可见成团的鹅卵石状室管细胞隆起于表面，微绒毛更为丰富，微绒毛根部可见到若干微细小孔，在SFO体部的一些室管膜细胞的胞膜完全破裂，胞质几乎荡然无存，仅剩胞核居于细胞的一侧，直径有5.7μm，呈分裂叶状；与这些细胞邻近的室管膜细胞表面最高处多呈现皱襞。2小时与16小时的实验组的观察结果与对照组相比未发现明显差异。

There was significant difference in T lymphocyte proliferation between the TF treatment groups and the control groups (p＜0.01), and between TF from black-bone silky fowl and other treatment groups (p＜0.01). Significant difference was detected between TF from black-bone silky fowl and homogenous Cherry Valley duck (p＜0.01). The effect on T lymphocyte proliferation in vivo was markedly enhanced when taking oral TF in 24 h, and significant difference remained between the treatment groups and the control groups in 240 h.

实验结果进行t检验分析，各实验组TF与对照组数据进行比较极显著差异，p＜0.01；乌骨鸡来源TF与其他实验组数据进行比较极显著差异，p＜0.01；T细胞体内转化增殖实验结果表明TF口服作用24 h内极显著提高T细胞转化增殖效果，而且240 h内T细胞的增殖效果与对照组相比仍然保持极显著差异。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。