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And it was divided into four groups at randomly (n=6),The vertical compression,bending and torsion intensity in without and with reduction of cortical fragment of plate fixated contra side, without and with reduction of cortical fragment of plate fixated sidewere measured.A11 results were analyzed with statistic method.2、animal experiment :The 12 adult goats were divided into two group at randomly,wedged cortical defect with 1 cm of long and conjugate diameter was created in the middle-part of the medial femur and plate was fixed in the defect side and as the experimental side,the other side was control.after operation 4、8、12 weeks,investigation of the fracture healing by means of gross observation,X-ray examination and histology in both sides.

测试各组的垂直压缩、三点弯曲及抗扭转的刚度。2、动物实验:12只健康成年山羊,雌雄不限,体重在20~25kg之间,在同一只羊双侧股骨中段内侧制造楔形骨皮质缺损,分实验组和对照组两组进行钢板内固定并植骨,于术后4、8、12周对实验组及对照组行X线正位片检查,观察骨折愈合过程中不同时间点的影像学特点。于4、8、12周各组处死4只动物行大体标本观察,并取缺损区骨组织行组织学检查。X线检查及组织学检查结果根据LaneX线评分标准及组织学评分标准进行评分。最后采用SPSS13.0进行统计学分析。

According to the corneal thickness measured, a 7.0mm Hessburg-Barron corneal trephine was used to incise cornea into different deep defect in respective experimental group and placed 7.75mm SIS implant over the corneal defect.The implant was continuous sutured over the defect.The reaction of white rabbit corneal was observed and recorded everyday within postoperative observation period constituted 105 days.

根据所测量的家兔角膜厚度,使用直径7mm的HessBurg-Barron负压环钻分别对三组实验家兔的角膜植床取不同的深度,植入直径为7.75mm猪小肠黏膜下层,并将其连续缝合于植床上。

No Ⅲ° atrioventricular conduction block happened, 9 patients developed right bundle branch block, and 2 patients showed junctional rhythm. Two patients with perimembranous extending outlet were repaired because of residual shunt(0.4cm,0.3cm) on the anterosuperior rim of defect. One patient with trivial residual shunt(0.15cm) on the posteroinferior rim of defect was found closed spontaneously six months later. Tricuspid valve was incised in 10 patients of VSD sextending outlet,8 patients vertical to the valve ring,2 patients parallel to the valve ring. Moderate tricuspid valve regurgitation was found in 1 patient, mild in 5 patients, trivial in 4 patients. No one aggravated. One patient complicated with pericardial effusion.

无Ⅲ°房室传导阻滞发生,9例发生右束支传导阻滞,2例结性心律。2例膜周偏流出道型VSD患者因前上缘残余分流分别为0.4cm和0.3cm,再次手术修补;1例后下缘残留细束样分流,直径0.15cm,随访6个月后自愈;10例膜周偏流出道型VSD患者剪开三尖瓣,8例垂直瓣环,2例平行瓣环;1例三尖瓣中度反流,随访无加重;5例轻度反流,4例轻微反流,随访均无加重;1例心包积液;1例再次进入手术室止血。

objectiveto explore the application of carpometacarpal superficial vein graft in the digital replantation.methodthirty cases hospitalized in our hospital from january 2004 to october 2008 were dealed with vessel defect in the digital replantation by carpometacarpal superficial vein graft.resultsthe replanted fingers all survived,the appearance and function were good.conclusionusing carpometacarpal superficial vein graft is a good way to deal with vessel defect in the digital replantation.

目的:探讨腕掌侧浅静脉移植在断指再植中的应用。方法:对30例患者采用腕掌侧浅静脉移植处理断指再植手术中的血管缺损。结果:再植手指均成活,外观和功能良好。结论:对于断指再植中血管缺损的处理采用腕掌侧浅静脉移植是一种很好的方法。

Results The long term results of the vascularized fibular graft varied in different types of bone defects. Nine patients with congenital pseudoarthrosis of tibia had the limb function restored in 65% and one had nonunion of fibular graft, 3 fractures(7 times), and 5 angular deformities. Three patients with congenital radial deficiency had 62% restoration of the limb function. All of them showed early closure of epiphyses of the grafted fibulae. Five cases with osteomyelitis and bone defects combined soft tissue deficiency had 94% restoration of the limb function, and only one occurred fracture of fibular graft. One case with soft tissue defect and concomitant ulnar defect, ulnar nerve and median nerve injury, 67% function of limb was recovered, and union of fibula graft was good. 100% function of limb was recovered in 1 case with cavernoma of radius and without occurring valgus deformity of donor site.

结果应用带血管腓骨移植修复骨缺损的远期效果因缺损类型的不同而存在较大差异。9例先天性胫骨假关节患者,术后肢体功能恢复65%,其中移植腓骨骨不连1例、骨折3例(7次)、成角畸形5例;3例先天性桡骨缺如患者,术后肢体功能恢复62%,3例术后均出现移植腓骨骨骺线早闭,畸形复发;5例骨髓炎、骨外露、骨缺损并软组织缺损患者,术后肢体功能恢复94%,仅1例发生移植腓骨骨折;1例前臂软组织缺损并尺骨缺损,尺神经、正中神经损伤者,术后肢体功能恢复67%,骨愈合顺利;1例桡骨海绵状血管瘤者,术后肢体功能恢复100%,骨愈合顺利。

Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.

并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只),移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞,对照组注射PBS。

To obtain more information of magnesium homeostasis in mitochondria, mTn-lacZ/LEU2 transposon library was transformed into mrs2 deletion mutant to screen for suppressor genes of MRS2. YMR166C, a member of mitochondrial carrier family, was identified as a suppressor gene of MRS2. Deletion of YMR166C gene can rescue the defects of mrs2 deletion mutant such as the decrease in mitochondrial magnesium concentration, Group II RNA splicing defect and growth defect on nonfermentable carbon source. For the first time we demonstrated YMR166C is involved in mitochondrial magnesium homeostasis.

为了增进对线粒体镁离子代谢调控基因的了解,利用酿酒酵母mTn-lacZ/LEU2转座子文库筛选MRS2的抑制基因,发现线粒体载体家族成员YMR166C基因的缺失可以挽救MRS2基因缺失的突变体的生长缺陷、II型内含子剪接缺陷,并可以调节线粒体镁离子浓度,首次发现YMR166C是线粒体镁代谢相关基因。

The mechanisms of bone defect repairing by DBM+bBMP composite are realized by bone induction and conduction in the defect area, and its capacity of repairing bone defect is better than pure DBM. It is an ideal and effective osteogenetic implant substitute.

异体DBM复合bBMP材料通过骨诱导和骨传导两种方式修复骨缺损,其修复骨缺损的能力要优于单纯DBM材料,是一种较为理想,具有高效成骨活性的植骨材料。

BACKGROUND: Facial nerve defect seriously impacts the mental and physical health of patients as well as their quality of life. Pediceled neck muscle transplant and autologous nerve transplant have been used in clinical to repair facial nerve defect and restore the facial muscle function. However,there are disadvantages and limits of above methods which have restricted their wide application of them. It is a research hotspot for looking for a new material to repair facial nerve defect.

背景:面神经缺损严重影响患者身心健康及生活质量,临床采用较多的有带蒂颈肌移植术和自体神经移植术等修复面神经缺损,恢复面肌的功能,但上述方法均有其缺点和局限性,使其广泛应用受到限制,寻求一种新的修复面神经缺损的材料是目前研究的热点。

Of the 32 articles, 4 were about the selection of destination genes, 3 about the selection of target cells, 7 about the selection of gene expression vector, 3 about the methods of gene transfer, 4 about gene therapy combined with tissue engineering in treating bone defect, 5 about polygenes in treating bone defects, and 2 about the problems and prospects in the gene therapy of bone defect.

符合纳入标准的32篇文献中,4篇涉及目的基因的选择,3篇涉及靶细胞的选择,7篇涉及基因表达载体的选择,3篇涉及基因转移方法,4篇涉及基因治疗与组织工程结合治疗骨缺损,5篇涉及多基因联合治疗骨缺损,2篇涉及骨缺损基因治疗存在的问题与展望。

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This one mode pays close attention to network credence foundation of the businessman very much.

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