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FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3 mediated by PEG/LiAc and by electroporation . In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .

把FDR3基因片段克隆到酵母表达载体pDBLeu上,然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体(M3 mutant)中,采用酵母异源互补法进一步鉴定FDR3基因的功能。

In order to overcome defect of traditional koumiss, the technology for the koumiss processed from juice mixed with milk peptides hydrolyzed by protein enzyme was studied by orthogonal methods and contrast tests.

为克服传统乳酒缺陷,该文采用蛋白酶水解法及酒精酵母乳酸菌混合发酵法研究了牛乳蛋白多肽复合果汁乳酒的生产工艺。

Magnetic flux leakage detection technology is an useful method in the online defect of the ferromagnetic oil-gas pipelines.

漏磁检测技术是铁磁性油气管道在线缺陷检测的有效手段。

In clinical, it has been found that treatment related to cleft palate are ineffective or invalid, because of unsuccessfully regenerating the bone defect of palate.

在临床上也发现由于腭部缺少硬组织的支持,腭裂其它相关治疗常常难于奏效或者疗效不巩固。

In contrast to the existing recoding system, this system has simple structure with lower cost, and the function of satellite simulator makes up the defect of existing recording system.

与现有记录系统相比,该系统具有结构简单、造价较低的优点,同时其卫星模拟源的功能也弥补了现有记录系统的不足。

The reason that caused the common air leakage defect of the aluminum alloy wheel hub with web spokes in production was analyzed using CAE technology, and the improvement scheme of the mold for wheel hub gravity casting was proposed.

针对筋条呈网状的铝合金轮毂生产中常见的漏气缺陷,运用CAE技术分析了这种漏气缺陷产生的原因,提出了轮毂重力铸造模具的改进方案,并用CAE仿真验证了该方案的正确性,实际生产统计数据表明采用该方案有效地降低了网状铝合金轮毂产品的漏气率。

According to the defect of library buildings in China——lack of cul tural taste, this paper puts forward the concept of cul

针对我国图书馆建筑的缺陷——缺乏文化品味,提出图书馆建筑文化性这一概念,剖析其深层的内涵,并初步探讨就其在图书馆建筑中的意义。

The defect of legal thought in the Confucian manner is that, on the one hand, it overlooks the difference between morals and law while, on the other hand, due to the exaggeration of their difference and failure to admit their connection morals and law are seen as uncompromisingly conflictual.

孔子信徒地法律上想法的缺点是一方面,它忽略道德和法律之间的差异消磨另一方面,他们的差异的夸张和未能容许他们的联系由于道德和法律被视为不妥协地战斗的。

This effective way of operation can be widely applied in many types of external ear deformities restoration, especially for the patients who have full thickness defect of external ear.

耳后乳突区皮瓣可广泛用于耳廓多种类型畸形的整复,尤其对于有外耳全层组织缺损者,是一种行之有效的手术方法。

Methods : The defect of bone, skin and vessel in the bone and joint of limbs was treated by microsurgical method and the bone and joint injury was repaired by the external fixation supporter of odjustment cross joint with three Kirschner wires, keeping the free motion of every joint.

在四肢关节部位有骨缺损或骨折伴有血管损伤、皮肤缺损等病例,应用带血管的骨关节块移植、游离血管移植或各种皮瓣移植等显微外科方法修复后的同时,用三针可调外固定器跨关节外固定治疗骨关节损伤,保持各关节的自由活动。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。