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Coli BL21 and purified. The binding activities of immune sera to three EDs were examined with ELISA and Western blotting analysis.②Asthma model was induced with ovalbumin in mice of vaccine group, control vector group and asthma group. Airway pressure-time index and number of cells in BALF were checked. The quantity of goblet cells in bronchiole and the level of MUC5AC mRNA in lung were investigated by PAS staining and RT-PCR assay.

采用ELISA和Western blotting法检测免疫血清抗体对ED的亲合力。2、对DNA疫苗组小鼠制备OVA致敏哮喘模型，检测气道压力峰值—时间指数、支气管／肺泡灌洗液中炎性细胞数量，PAS特染观察小支气管杯状细胞数量及RT-PCR法检测肺组织中粘蛋白MUC5AC mRNA水平，免疫组化和RT-PCR法检测GM-CSF的蛋白与mRNA水平。

Of course, Switzerland, with its compulsory military service, state controlled monetary system, railroad and telephone services, and taxation, is not a pure libertarian society but for those interested in reining in out-of-control governments in other parts of the world, there are large parts of the Swiss cantonal system that are worthy of emulation.

当然，瑞士因为其强制征兵、州立财政系统、州立铁路、电话和税收等，并非一个绝对的自由主义的社会－但是对于世界其上很多仍然在寻求控制其"无法无天"的政府的地区，瑞士的州立系统是有很多值得效法的地方。

About countrywide salary the key word of average level sort is in recently of Baidu collect an amount big ever, everyday 100 thousand is control, in SEO tall person carry bit, preparation is in second what to do on key word.

最近关于全国工资平均水平排序的关键字在百度的收录量大曾，每天有10万左右，在SEO高人的提点下，准备在次关键字上做点什么。

Based on Bezier curve continuance of first－order and second－order derivative, the characteristic vector equation of two Bezier curve is presented in point of intersection. The algorithm is not only of high accuracy with all of the interpolated points being on the curve theoretically, but also of very high speed in the real－time interpolation processes. The interpolation velocity does not change and the segment tolerance of interpolation can be control by selecting the parameter in cerement rationally every time.

为保证两段Bezier曲线在连接点处一阶导数、二阶导数连续，导出了两段Bezier曲线特征向量应满足的关系方程，Bezier插补法不仅在理论上可使所有的插补点落在曲线上，而且实时插曲补速度高，每次插补时可合理地选择参数增量，能使插补速度保证不变，也可控制弓高误差。

Being more intelligent, easy of integration, friendly in man-machine interactivity and adaptable to dynamic environment, this new controller fits in well with cellular manufacturing systems with characteristics of distribution, autonomy, coordination and cooperation in control and changefulness in organization.

这种新型控制器具有更高的智能、良好的集成性、友好的人机交互以及强的动态适应能力，很好地适应了敏捷单元化制造系统中各制造单元之间分布自治、协同合作的生产控制特点和动态多变的组织特点，为制造系统集成和多单元、多地点协调协作提供了良好的技术基础。

Modal analysis of an acoustic system can determine its natural frequency and show the distribution of the acoustic quantities in space, give the knowledge about the way that the acoustic system responses are affected when changes are made in the excitation sources or in the domain geometry, and provide valuable information for the noise control of closed three-dimension domain.

通过对声学系统的模态分析，可以得到其固有频率以及在此频率下声学量在声场空间上的分布，了解到声源及声场空间几何参数的变化对声学系统的相应的影响，为三维封闭空间的噪声控制提供了依据。

In tussle process, 3 market control of industrial and commercial bureau member the back of hand and small arm are slight abrade, wang Mou is in parenchyma to contuse more all over, flower of be in hospital expends more than 1000 yuan with medical treatment.

厮打过程中，工商局的3名市场治理员手背及小臂稍微擦伤，王某全身多处软组织挫伤，住院花用医疗费千余元。

Results It is found that the prevalence rates of chronic pharyngitis and coryza in workers were higher than in children or controls, those of dental fluorosis in workers or children were higher than in control while the SIgA was opposite.

结果 工人咽炎和鼻炎高于儿童及对照区，工人、儿童氟斑釉高于村民对照组而SIgA低于村民对照组。

He life space of family caregivers turns out to be in covariation with that of people with dementia. In order to take control of caregiving and living requirements, most family caregiviers would modify the life space of the elderly carereceiver. In this modification process the caregiver's own life space also undergoes changes to different extents.

属和失智老人的生活空间属於高度相关的共变关系；大多数家属会调整老人的生活空间，好让自己得以掌握大部份的基本生活及照顾事务，少部份家属由於两人关系较难著力改变老人的生活空间，因而转以调整自己生活空间的方式，或是引入社会邻里等资源的协助，来因应不断变化的照顾需求。

Results On postoperative day 10, the cumulative nitrogen balance and the level of plasma fibronectin was significantly higher in rhGH group than in the placebo group (P 0.05). Furthermore, on 4 d and from 6 d to 9 d after operation, daily nitrogen balance was higher in rhGH group than in control group(P 0.05). Mean blood glucose levels were significantly higher in the rhGH group from 3 d to 6 d after operation (P.05). But hyperglycemia could be well controlled by insulin treatment.

结果 术后第10 d，rhGH组累积氮平衡和血浆纤维连接蛋白水平优于对照组（p＜0.05）；术后第4 d和6～9 d，rhGH组每日氮平衡均优于对照组（p＜0.05）；rhGH组治疗期间血糖水平升高，但程度较轻，可为胰岛素有效控制，未观察到严重并发症发生。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。